We narrowed to 241 results for: p
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TypeBlog Post...incorporated into the full-length protein. Up to 80% p-Ser incorporation into E17TAG GFP has been achieved...incorporation due to differences in stability of the p-Ser incorporated protein as well as context-specific...
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Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature...with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet ... -
Management for Scientists: Seeking Feedback
TypeBlog Post...Use active listening skills such as the PAC Method. P = demonstrate patience, pause; A = ask at least one... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post... References and Resources References Andrews, N. P., Boeckman, J. X., Manning, C. F., Nguyen, J. T., ...protocols.io.bs68nhhw Im, K., Mareninov, S., Diaz, M. F. P., & Yong, W. H. (2019). An Introduction to Performing... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post..., De Los Angeles A, Mali P, Aach J, Kim-Kiselak C, Briggs AW, Rios X, Huang P-Y, Daley G, Church G (2013... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...doi.org/10.1038/s41477-020-0704-5 Santana I, Wu H, Hu P, Giraldo JP (2020) Targeted delivery of nanomaterials...doi.org/10.1038/s41467-020-15731-w Wang F, Wang C, Liu P, Lei C, Hao W, Gao Y, Liu Y-G, Zhao K (2016) Enhanced... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...Staber, S. J. Wheelan, J. K. Joung, P. B. McCray, F. D. Bushman, P. L. Sinn, and N. L. Craig, “piggyBac... -
Easing the Protein Purification Process with pCri
TypeBlog Post...producing difficult-to-fold proteins. In addition, P. pastoris has the capacity to glycosylate proteins... -
Reflections and Looking Ahead
TypeBlog Post...Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable, H., & Longworth... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...loci in human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post...CRISPR-Guide RNA Library. Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...Hurtrel, B., Heard, J.-M., Rivière, Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent... Hauswirth, W. W., Boye, S. E., & Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...797459. Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P, Vartak NB, Brown M, Talbot D, Berg DE. pBR322-derived...Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of Circular Plasmids... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post... https://doi.org/10.1089/hum.2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong, A. C. H... Emily P. Bentley... -
Using AAV for Neuronal Tracing
TypeBlog Post... Bohn, P., Joines, K.M., Peng, H., Hawrylycz, M.J., Phillips, J.W., Hohmann, J.G., Wohnoutka, P., Gerfen....L., Kieran, D., Redmond, D.E.Jr., and Aebischer, P. (2010). Efficient transduction of non-human primate... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post... https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020. ... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post... https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...Zhang, Y., Dong, A., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin,... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...4594039. PubMed Central PMCID: PMC427208. Novick, R P et al. “Uniform Nomenclature for Bacterial Plasmids... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...vectors The vast majority of fly vectors are based on p-element, random or targeted, integration into the ...