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Showing: 121 - 140 of 291 results

  4. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...rXRCC1, or the DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion... proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding...

  5. COVID-19 Resources

    Type
    Collection
    ...window) reverse transcriptase, protease, and Bst polymerase constructs now available from Andrea Pauli's ...Diagnostic Toolkit Enzymes. (unpublished) Taq polymerase and MMLV reverse transcriptase expression plasmids...

  8. Bacterial Expression Systems

    Type
    Collection
    ...with a 6x His tag. Requires a strain with T7 RNA polymerase. Amp resistant. More expression vectors for protein... of the pB1H2w derivatives as a fusion to RNA polymerase subunit ω. Transcription factor binding to a ...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...Michael Rosbash 128046 pGL3basic luciferase and renilla_polyA Firefly/Renilla Insertion of 5' promoter/enhancer...luciferase James Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression of firefly luciferase from...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mRFP-Clc Clathrin-coated vesicles Clathrin, light polypeptide mRFP Ari Helenius 70217 pEGFP-GFP11-Clathrin ...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 Early endosomes...

  11. Cre-lox system

    Type
    Collection
    ... metallothionein MT-I region including the polyadenylation site and several introns tet-hCMV Mammalian...Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA mCherry and Cre; expressed in excitatory neurons...

  12. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...

  16. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls Feng New viral service, ...

  20. Reflections and Looking Ahead

    Type
    Blog Post
    ...R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A. I., Saper, C. B...
Showing: 121 - 140 of 291 results