We narrowed to 290 results for: poly

1. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   ... challenges of science becoming increasingly politicized. It would be nearly impossible for a career scientist...science in a “fake news and post-truth era,” the politicization of science (from climate change deniers to ...

2. Transferable Skills Guide: Creativity

   Type

   Blog Post

   ...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

3. Plasmids 101: Repressible Promoters

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   Blog Post

   ...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...guide to working with Drosophila Terminators and PolyA Signals FLEx Vectors Resources at Addgene.org ...

4. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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   Blog Post

   ...Wednesday we worked with the Harvard GSAS Science Policy Group to organize a Minisymposium on Reproducibility.... Down the line we may see further adoption of policies that promote reproducible publishing practices...

5. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   ... in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function... in Biology and Biotechnology from Worcester Polytechnic Institute where he investigated how plants achieve...

6. Quick Guide to All Things Lentivirus

   Type

   Blog Post

   ... you will find will recommend using a polycation, like polybrene (protocol) or DEAE dextran (protocol)...

7. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   Blog Post

   ...105 colonies. Pro Tip: Use a high-fidelity Taq polymerase with proof-reading ability for generating dsDNA...nucleotides 1 out of 104 to 105 Use a high fidelity Taq polymerase to generate insert ssDNA Point mutations or...

8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   Blog Post

   ...binding to the template strand (strand used by RNA polymerase II for transcription) are more effective for ...immunity and mammalian genome editing via RNA polymerase-mediated dislodging of Cas9 from double strand...

9. CRISPR 101: Cytosine Transversion Editors

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   Blog Post

   ...rXRCC1, or the DNA binding and lyase domain of DNA polymerase β (PB) (Chen et al., 2021). Both of these fusion... proteins whose fusion improved the CGBE: DNA polymerase D2 (POLD2), exonuclease 1 (EXO1), and RNA binding...

10. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type

    Blog Post

    ... development, business development, consulting, policy, big data and the list goes on and on. As you think...Hire American" Executive Order which sets broad policy intentions directing federal agencies to propose...

11. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    Blog Post

    ... or a dCas9 fused to the omega subunit of RNA polymerase, the lab   successfully repressed or activated... al., 2014). These could be genes, specific polymorphisms, including antibiotic resistance genes. This...

12. Bikard Lab - CRISPR Repression Collection

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    Collection

    ... a gene, it blocks the progression of the RNA polymerase, thus strongly decreasing the expression level...

13. Synthetic Biology - Overview

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    Collection

    ...Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters Anderson Lab Plasmids and Phagemids...

14. Control AAV Preps

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    Collection

    ...Deisseroth 192552 pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA CAG tdTomato Constitutive 9, PHP.eB Feng 27056...

15. Ras Pathway

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    Collection

    ...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...

16. TALEN Plasmids and Kits

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    Collection

    ...specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View...

17. Zinc Finger Consortium: Zinc Finger Arrays

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    Collection

    ... OPEN OPEN cACCCTCCTCgtggtGCTGGTGGCc apoeb (apolipoprotein Eb) OZ529 and OZ530 OPEN OPEN gCCCCTCAGCccagatgGGAGGAGATg...

18. Distribution to Industry

    Type

    Collection

    ...be processed and shipped. Industry Distribution Policy A portion of Addgene's collection is available ...

19. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ...of assembling these multi-protein fusions or “polyproteams”, the Howarth lab created the MBPx-SpyCatcher...sequential additions have been made, the final polyproteam can be eluted using maltose. This procedure allows...

20. Neurodegeneration Research Collection

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    Collection

    ...have huntingtin proteins with abnormally long polyglutamine sequences. These glutamine-rich sequences are...environmental factors. For example, variations of Apolipoprotein E (APOE), such as the ε4 allele, are a risk...