...efficiency of Cas9 cleavage is relatively high while the efficiency of HDR is relatively low, meaning that most...Anzalone et al., 2019). PE3, as originally introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch...act first. PE4 and PE5, as originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor...

... generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail.... The advantages of this method are that it’s relatively simple to implement and doesn’t require time-...Overall, integrating viral vectors efficiently and reliably generate iPSCs, but they have several safety concerns...important control. Additionally, Sendai virus doesn’t rely on promoters for transcriptional regulation, so ...Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate the Stem...

...binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins containing...or function of a single protein? Oftentimes, they rely on protein purification. In this strategy a single...contrast, the polyhistidine tag is very small and rarely affects protein function (Bornhorst 2000).  One...other affinity purification methods where binding relies on a specific protein conformation, the polyhistidine-metal...questions about polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody...

...our engineers (Fig. 2). Creating this guide was relatively easy. I took a picture of virtually every container...each given container, when applicable Any other relevant information Prescribing an official name to all...important documentation, including SOPs or project-related documents, we define new and potentially ambiguous...transferable skills Find all of our science career related blog posts Read our science communication blog ...

...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

...vector systems Gamma-retroviral vectors have a relatively high transduction efficiency, but in contrast...

...widefield imaging fluorophores are excited with relatively low light levels (for a prolonged time). Other...illumination, TIRF or spinning disk confocal use entirely different excitation power regimes. Therefore,...experiments. As the photobleaching rate is not linearly related to power, the conclusions reached at high power...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...water/oil emulsion at high power, it is far more relevant to measure how fluorescence intensity evolves ...

...measurement. Like a p24 ELISA, this method is relatively quick, providing results in less than a day, ...

...structure, constructing split variants should be relatively straightforward, but it is also possible to create...

...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...called TOPO® cloning or TA cloning, is a method that relies on the hybridization of the complementary base ...Independent Cloning Ligation Independent Cloning (LIC) relies on the 3'-5' exonuclease activity of T4 DNA polymerase...

...identical antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing...revealing the unreliability of antibodies. There are three main underlying causes of this unreliability: cross-reactivity...sifting through comments and papers to find the most reliable (and cost-effective) antibody for my experiment...the antibody can no longer bind to the target). Unreliable antibodies cause research that cannot be reproduced... of all commercially available antibodies are unreliable (Baker, 2015), causing a huge waste of precious...specific (minimal binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible... thousands of scientists to confidently choose reliable and consistent antibodies for specific markers...

.... insulin). Proteins, unlike DNA which can be relatively easily synthesized, must be produced using complex...protein expression, S. cerevisiae, is the most reliable and frequently used species due to its use as ...

...If the phenotype is corrected, students can be relatively confident that the gene from the test species...

... are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One ...These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but...transient PPIs. Many PPI identification techniques rely on protein interactors to stay in close contact ...

...support scientists wear many hats at this still relatively small nonprofit. If you do go longer than a few...these tips and tricks will apply to any biology-related customer support interaction. Before contacting...

...City The conference was located in the modern, relatively new “Science City” area on the outskirts of Guangzhouon...Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These single stranded, covalently...of Georgia studies the CRISPR/Cas system as it relates to its natural role in prokaryotic immunity to ...

...While this method can be time-consuming, it is relatively effective in reducing off-target effects since...

...agarose gel to confirm product presence, size, and relative quantity. End point PCR is most commonly used ...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...

...attending the session, and what is their background relative to your research? Tailor your language to your... you may receive. People often ask about the relationship of your work to recently published papers or...audience just that. Explain what you do know about a related topic, and let them know while you appreciate the...collaborate with a group who presented on a topic related to your research? Find a way to get in contact ...

... 100-fold excess of wild-type bacteria and the relative frequency of each of the 40 library strains was...