We narrowed to 303 results for: rigi
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TypeBlog Post... Dr. Rod Devenish (both at Monash University) originally developed the system for monitoring and analysing...prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen et al 2013) allow for... with an appropriate phosphatase, a technique originally pioneered by Markus Seeliger for the high-yield...
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Important Considerations When Using AAVs
TypeBlog Post...which cell type? Location (3): Traveling from the original location - Axonal Transport in the CNS What tissue...your particular experiment! Traveling from the Original Location - Axonal Transport It has been demonstrated... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...simplest and smallest bacteria. The absence of a rigid cell wall makes them resistant to antibiotics and...its ability to change shape and the absence of a rigid cell wall. Here, I will cover some of the best ways... -
Typing CRISPR Systems
TypeBlog Post... and variants are typically based on species of origin. There are currently two classes, six types, 33...s0006297921100114 Koonin, E. V., & Makarova, K. S. (2019). Origins and evolution of CRISPR-Cas systems. Philosophical... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...acceptors, then biarsenicals are a good option. Originally developed by Roger Tsien and co-workers (Griffin...compounds such as British anti-Lewisite (which was originally developed to treat heavy metal intoxication in... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...you to quickly associate your plasmids with the original publication where you described them. Search for...available to the research community. This article was originally written by Tyler Ford in January 2016. It was... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...journal editor) of the work of those in your field. Original contributions to the field that have had a major... the same or allied academic field Evidence of original scientific or scholarly research contributions... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...This pEcCas/pEcgRNA system works similar to the original pCas/pTargetF but has many other advantages including... -
15 Hot Plasmids from 2017
TypeBlog Post...Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan... variant HF-Cas9. They subsequently tested the original BE3 and HF-BE3 using a gRNA with high off-target... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...contextualized knowledge of their roles inside cells. The original APEX (enhanced ascorbate peroxidase) reporter ...their canonical positions (SP218, SP222) using the original or improved phosphoprotein synthesis reagents.... -
Antibodies 101 Series on TikTok
TypeBlog Post...antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post...* Aavery! Welcome to Addgene, Aavery! Original post: In December, we introduced you to Blugene... -
CRISPR Cheat Sheet
TypeBlog Post...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp... -
Our New Antibody Mascot is....
TypeBlog Post...unveil the winning name on Feb. 24. Stay tuned! Original Blugene and Aavery would like your attention, ... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog Post...we wouldn’t necessarily have expected when we originally sent out the call for applications. Cervical ... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog Post... By John Doench (Guest Blogger) Plasmids 101: Origin of Replication By Kendall Morgan Save Time and... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...synthetic vector that can have a plasmid or viral origin. The type of cloning approach you choose ultimately... -
Your Top Requested Plasmid in 2015!
TypeBlog Post...2015 was... lentiCRISPR V2 LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...genome of a group of Archaea. Consistent with this origin, the authors suggest that Cas14 could be a more...