...Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN...

...Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID...

...Science 2011). Follow up work from the Rinehart and Issacs labs at Yale have made improvements to this system...

...including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3 [N202/7R] Anti-Ankyrin-G [N106/36] Generate anti-kinesin...

... Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing and non-dividing cells...

...also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged...

...cloning, and a "stuffer" fragment that includes the SacB gene, allowing for negative selection on 5% sucrose...

...azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted...

...pyogenes Jaenisch pU6_gRNA_handle_U6t 49016 Mammalian SacI none S. pyogenes EBFP Lu pH1v1 60244 Mammalian Gibson...

...vectors are based on Ad5. Ad5-based vectors use the Coxsackie-Adenovirus Receptor (CAR) to enter cells. Recombinant...contain GFP Additional Vocabulary Term Definition Coxsackie-Adenovirus Receptor (CAR) Receptor used by Ad5...

...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

...class II, major histocompatibility complex, transactivator C2TA, CIITAIV, MHC2TA, NLRA CLEC4M C-type lectin...

...use in AAV, or containing Staphylococcus aureus (SaCas9) . AAV is also a promising method for gene therapy...

... Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing and non-dividing cells...

...this promoter is no longer dependent on Tat transactivation. The 3rd generation transfer plasmid can be...