We narrowed to 129 results for: sacs
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TypeBlog Post...evolution of CRISPR-Cas systems. Philosophical Transactions of the Royal Society B Biological Sciences, ...
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With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...nightmare that is Times Square. But I made the sacrifice in April because devaluing science has alarming... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School.... -
Genetic Code Expansion
TypeCollection...azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pyogenes Jaenisch pU6_gRNA_handle_U6t 49016 Mammalian SacI none S. pyogenes EBFP Lu pH1v1 60244 Mammalian Gibson... -
CRISPR Guide
TypeGuide...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1... -
Adeno-associated virus (AAV) Guide
TypeGuide...has been to use smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus . The Feng Zhang lab...lab has developed plasmids containing SaCas9 optimized for their use in rAAV vectors . Read our blog post... -
Lentiviral Vector Guide
TypeGuide...often CMV or RSV), eliminating the need for transactivation by Tat. Most third-generation transfer plasmids... -
Immunology Research Plasmids and Resources
TypeCollection...class II, major histocompatibility complex, transactivator C2TA, CIITAIV, MHC2TA, NLRA CLEC4M C-type lectin...