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We narrowed to 325 results for: sars

Showing: 121 - 140 of 325 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our...following our blog, you may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply ...

  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells ... it. Here, we'll cover the promoter, which is necessary for getting the plasmid express our gene of interest...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...theoretical maximum value, physical titers don’t necessarily give any indication of the infectious titer. ...and convenient. However, these methods are not necessarily precise or accurate for quantifying AAV because...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ..., though – someone accepting your CV doesn’t necessarily mean that you are a future employee of the company...might even be a colleague. A mentor does not necessarily need to be someone a lot older than you are; ...

  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...an expression clone with all of the components necessary for gene expression.     The LR Reaction ...all kind of organisms from mammals to E. coli. Glossary of Gateway cloning vectors Vector type  Vector...

  7. Adenovirus Guide

    Type
    Guide
    ...2021). Adenoviral vector vaccine platforms in the SARS-CoV-2 pandemic . NPJ Vaccines, 6 (1), 97. https:... Common Uses Safety Resources Plasmid Elements Glossary Adenoviruses (AdV) are medium-sized, non-enveloped...virus enters the host cell, and encode proteins necessary for viral DNA replication and host cell manipulation...vectors or simply need a refresher, we've included a glossary at the end! Browse Adenoviral Plasmids Adenovirus... containing the rest of the adenoviral genome necessary for viral production and homology regions for ...containing the ccdB gene, a ccdB-resistant strain is necessary. For more information on cloning and working with...e.g. pAdEasy) — containing the adenoviral genes necessary for viral production. In AdEasy™, the transgene...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly monomeric EGFP variant...Hink et al., 2000). The complete beta-barrel is necessary for its fluorescence and therefore GFP cannot ...

  11. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...prevent cutting at mismatched guide sites, but not necessarily binding to these sites. Thus, these enzyme swaps...perform whole genome sequencing. While this may be unnecessary for many applications, it is the only comprehensive...

  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Systems Together You may be wondering, “why is it necessary to have all 3 of the different binary systems ...similarly?” The simple answer is that sometimes it is necessary or desirable to control or look at multiple genes...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...knockdown or overexpression experiment, generate the necessary constructs, and complete screening in just 3 days...This toolkit reduces the expense and workload necessary to produce vectors for genome editing in worms...the initiation of the T. gondii infection, and necessary for the asexual cycle of malaria parasites. Find...

  14. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...missing? For example, are additional controls necessary? As you build your story, are additional data ...important to have a plan to get to mechanism. Do the necessary tools exist to tackle the problem and if so, do...

  15. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...packaging plasmid containing all the structural genes necessary for the production of a viral particle (Gag, Pol...encoding the insert of interest as well as sequences necessary to encapsulate that insert into a viral particle...

  16. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...the antibody you expect it to produce is not necessarily what you are getting. Learn more about monoclonal... Because the entire antibody molecule is not necessary for antigen binding, the variable regions alone...

  18. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...sections and 3D cultures These samples do not necessarily require optical sectioning, but open the door...by pixel are unable to provide the frame-rates necessary for imaging tissues of this size. For example,...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...work? What are the various tools and components necessary to run it? And, most importantly, what are the...lab focuses primarily on behavior, you don’t necessarily need to use electrophysiology to prove that your...

  20. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...includes a lot of acronyms, so we’ve included a glossary at the end if you ever need to double-check your... closer than ever. References and Resources Glossary We introduced a lot of terminology in this post...
Showing: 121 - 140 of 325 results