We narrowed to 172 results for: tox

1. AAVs CREATed for Gene Delivery to the CNS and PNS

   Type

   Blog Post

   ...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

2. CRISPR Antimicrobials

   Type

   Blog Post

   ...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used...

3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   ...fluorescent dyes of the time was the fact that it was non-toxic and could be expressed in living cells, enabling...

4. Bacterial Expression Systems

   Type

   Collection

   ...Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and visualization inside living...

5. CRISPR Pooled gRNA Libraries

   Type

   Collection

   ...2 plasmid) Knockout Human Moffat 3rd 4 70,948 Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A...

6. Rett Syndrome: A History of Research and Therapeutic Outlooks

   Type

   Blog Post

   ...the Survival of MeCP2-Null Mice without Apparent Toxicity after Intracisternal Delivery. Molecular Therapy...

7. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   ...did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller Cas proteins...

8. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   ...visualization technique. Ultimately, MG proved to be too toxic for many in vivo applications, but this work proved...

9. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   ...containing the ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach...

10. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    ...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...

11. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ... protein, which can be useful if the product is toxic in high amounts. These plasmids are also useful ...

12. 5 Reasons to Use Reddit for Science Communication

    Type

    Blog Post

    ...career topics. However, many of the posts were about toxic advisors, fruitless job searches, and even despair...

13. Plasmids 101: The Promoter Region – Let's Go!

    Type

    Blog Post

    ...even kill your cells if your gene of interest is toxic. To combat this, scientists have created synthetic...

14. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type

    Protocol

    ...macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other ...

15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    ...non-functional, they could be dominant-negative and/or toxic. A potential solution is to include an in-frame ...

16. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    ...for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked. ...

17. Five Popular Model Organisms

    Type

    Blog Post

    ...Yeast models of PD, like those that overexpress the toxic protein α-synuclein (the most common protein found...

18. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    ...imidazolinone (DFHBI). DFHBI is membrane permeable and non toxic to cells. Because it rapidly photobleaches, DFHBI...

19. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    ...display assays, ubiquitination activity assays, toxin activity, small molecule binding, growth rate, and...

20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    ...specificity, so in some cases they can lead to toxicity and off target effects. More recently, shRNA plasmids...