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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 962 results for: App;

Showing: 751 - 760 of 962 results

  1. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli - Recent...Physiology, Pathogenesis and Biotechnological Applications. InTech. https://doi.org/10.5772/67306 Koornneef...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...that expresses all desired variants of a protein. Applying a selective pressure winnows the pool down to ...technique described in Wrenbeck et al is a PCR-based approach for generating a customizable comprehensive mutagenesis...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments...protein module that can report on location. This approach enables the study of proteins in cells, but not...

  4. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help...budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz is a laboratory...

  5. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...protocols that you can use for a wide range of applications, with videos for select protocols in the right-hand... of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single reaction Ligation Independent...preparation Antibodies Protocols for common antibody applications. Name Description Link to Video Transfection...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...something odd in our uncut DNA control: a band or two appear on an agarose gel at notably higher molecular weights... only in the uncut DNA; the rest of the digest appears normal and produces the expected fragments for ...

  7. Using a Light Microscope Protocol

    Type
    Protocol
    ...see your image through both sides. Once you are happy with the lighting, use the coarse focus knob to ...make minor adjustments to the focus. After you are happy with the positioning and focus of your image on ...microscope after use and store the microscope appropriately....

  8. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...overnight culture of bacteria . Pro-Tip Refer to appropriate DNA prep protocol for volume of bacteria to grow...the plasmid DNA. Either spin to pellet the DNA or apply the solution to a column that will bind the now ...Aqueous DNA phase Middle phase - A white layer may appear, consisting of precipitated protein particles Bottom...

  9. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https...

  10. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immunotherapeutic agents. Currently the FDA has 12 approved monoclonal antibodies targeting the PD-L1 pathway...Dendreon Corporation is currently the only FDA-approved DC therapy for prostate cancer and is aimed at...
Showing: 751 - 760 of 962 results