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We narrowed to 545 results for: SEC

Showing: 141 - 160 of 545 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    ...and a line about what I hope to discover. This section may make the difference in whether you get funding... the successful use of your preliminary data to secure further funding. Make it clear that you intend ...your head up! It’s very likely that at first (and second) you won’t succeed, but you must try, try again...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...transcript.   Figure 1: A predicted conserved secondary structure and sequence conserved Rho-independent...contains enzymes that cut RNA not protected by secondary structure. It is thought that poly(A)s are used...example, relies on a specific sequence and RNA secondary structure to induce transcript cleavage, similar...

  3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ... to its final destination. As an example, most secreted proteins are produced in the endoplasmid reticulum...golgi and then transported in vesicles via the secretory pathway to their subcellular or extracellular ...Edition. 3.  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka...

  4. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...V1, with secondary peaks in V3 and V4, whereas BV711 peaks in the V13 channel, with secondary peaks in...101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies 101: Buffers, Storage, and...

  6. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...originally named orthogonal-plane fluorescence optical sectioning (OPFOS) (2). Arne Voie, David Burns and Francis...confocal microscopy In confocal microscopes, optical sectioning of a specimen is based on discriminating the ...semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire one image, thereby significantly ...

  7. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... grant readiness, identify grant opportunities, secure grant awards, and manage grant projects and funds... She has served in the nonprofit and government sector for 20 years, developing expertise in grant project...She and her team at J. Miller & Associates have secured more than $70 million dollars in grant funding....

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...primary target audience – colleagues in our field. Secondary audiences that may also benefit, such as researchers...research scientists, faculty, students, private sector researchers, or pretty much anyone applying the...funding, or are by researchers for whom English is a second or third language (making publishing their research...

  9. Bioinformatics at Addgene

    Type
    Blog Post
    ...linking the output from the first process to the second process. For example, our bbduk process created... declaration correctly, the script block in the second process printed one of the inputs, such as the ...copied a few lines of our existing script into the section of the next process. Similar to our first attempt...

  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...2014, the White House issued a memorandum to the Secretary of Labor to update the FLSA, which had not seen...automatically adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations...post by Director of the NIH Francis Collins and Secretary of Labor Thomas Perez, Fair Pay for Postdocs: ...

  11. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...Strong insect promoter from baculovirus Constitutive  Commonly used in expression systems for insect cells... promoter. Ac5 General expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive...

  12. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre...best site for the insertion is a loop in between secondary structure (beta-sheets or alpha helices). An additional...Joachim Goedhart is an assistant professor at the Section of Molecular Cytology and Van Leeuwenhoek Centre...

  13. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...desired codon changes (step 1 in the image above). A second universal primer is used to synthesize a complementary...strand. Each degenerate primer set contains three consecutive randomized bases (NNN) at a given codon site ...ExoIII and ExoI. Synthesize 2nd mutant strand: The second mutant strand is synthesized in the same manner...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...a gRNA in one AAV and a repair template in second a second AAV to deliver their repair template to mouse...are more appropriate for a CRISPR-based approach. Second, the AAV preps generally used for gene editing ...

  15. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...central steps: The 5’ DNA end of the break is resected by nucleases to create a ssDNA 3’ overhang. This... strand “captures” and synthesizes DNA from the second DSB end. The individual HJs are resolved via cleavage...importance for the repair of breaks that lack a second end to enable DSBR or SDSA.   Homology directed...

  16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... questions, please write to us in the comments section.  FX cloning kit This kit is designed to be used...expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The expression vectors...Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding your gene of interest...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...bacterial host strain for pSer-incorporation The second component necessary for phosphoserine incorporation...will allow full length GFP to be translated), and second, through a gel shift assay using the PhosTag system...Wisconsin. She is particularly interested in the intersection of cell signaling, metabolism, and the mighty...

  18. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ... we suggest estimating the time it will take to secure your first (R-level) research grant.  Set your ...?” As scientists, working with this question is second nature. Channel your inner curiosity and use your... be afraid to walk away from a negotiation. If securing a faculty position is your goal, then a resource...

  19. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...diffusible labels, or electron microscopy of stained sections, were limited due to low resolution or were very...fluorophores to aggregate in the neurons’ somata. Second, the system did not permit analysis by immunostaining...labeling has decreased the mapping time for a given section of brain by at least an order of magnitude. It ...

  20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...different time stamps in the description to watch any sections you’re particularly interested in, but I’d recommend...technique simply by following the abbreviated methods section found within a published manuscript. Luckily, many...a long way to encouraging researchers to take a second and think about how their biases affect their experimental...
Showing: 141 - 160 of 545 results