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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 386 results for: nans

Showing: 211 - 240 of 386 results

  2. Antibodies 101: Validation

    Type
    Blog Post
    ...non-mutant line but no signal in the mutant line! Recombinant Strategies This approach is essentially the opposite...ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  ...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer. For more information...

  5. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...more reproducible/renewable reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It...µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., ...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from... plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids...

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...AAV9 in the liver. AAV9-X1.1 effectively and predominantly transduces neurons across brain regions, with...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...

  9. New Coomassie Protocol Video

    Type
    Blog Post
    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...

  10. The 12 Days of CRISPR

    Type
    Blog Post
    ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...

  12. Deck the Lab 2022!

    Type
    Blog Post
    ...giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities ...

  15. Summer Fun at Addgene!

    Type
    Blog Post
    ...Instagram all summer long to see what kind of shenanigans we get into!  We hope you are as excited as we...

  21. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...followed by even splashier news: Jiahao Sha of Nanjing Medical University had successfully used the CRISPR...

  27. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...types the gene is expressed in and amount of recombinant protein obtained. Selectable Marker The antibiotic...

  29. Hot Plasmids - October 2022

    Type
    Blog Post
    ... catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is...

  30. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...training or one isn’t available yet, order a copy of Nancy Baron’s book “Escape from the Ivory Tower.”  9)   ...
Showing: 211 - 240 of 386 results