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We narrowed to 303 results for: hal.2

Showing: 281 - 303 of 303 results

  1. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...most important” tasks my schedule. This was bad for 2 reasons. First, it soon became obvious that I didn.... Personally I found this to be particularly challenging. As PhD students and postdocs we are used to ...

  3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...properties of the protein of interest can be challenging. In this blog I discuss strategies to generate... (catalytic activity or interactions) can be challenging. If a fusion can complement a knock-down or knock-out...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next time is ...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

  5. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...experience and to build upon your existing skills.  2. Find your niche Identify and develop an area of expertise...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

  6. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...replicate, then purify the DNA.     Figure 2: A pooled library page with protocols boxed in red...presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through how...

  7. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...) or the American Chemical Society (ACS) are just 2 examples of organizations that hold large, annual ...development (or lack thereof) One of the biggest challenges facing students today is that many graduate and...

  8. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...rate of 10-55% - the PASTE system.     Fig. 2: Schematic of programmable gene insertion with PASTE...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...

  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...effective recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced....the power of lethal mutation co-selection. Only animals rescued for the conditional lethal mutation survive...selection. Knock-in events tend to be rare, and the challenge with any experiment is in efficient recovery of...co-selection approach relying on repair of a conditional-lethal mutation to identify edits with minimal screening...screening effort. Selecting for repair of a lethal mutation should remove much of the unedited “haystack”, ...viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted in efficiencies...Engineering of theCaenorhabditis elegansGenome with Lethal Mutation Co-Conversion and Inactivation of NHEJ...

  10. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience in cancer research After graduating with a 2:1 BSc in Molecular Biology (roughly a B average in...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

  11. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...community is active, engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of...Getting on a good science career path can be challenging these days, but for many of us, scientist is ...

  13. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...components, see the AAV Plasmid Elements table . Figure 2: Plasmids required for rAAV vector production. Created... pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism...the central nervous system . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024...the production of AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384...retrograde access to projection neurons . Neuron, 92 (2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021...short coding sequences of channelrhodopsins, halorhodopsins, and other optogenetic genes makes them suitable...) and a gRNA together into an rAAV capsid is challenging due to its limited packaging capacity, especially...

  14. Chemogenetics Guide

    Type
    Guide
    ...muscarinic receptor and has not been used in vivo. Figure 2: DREADDs, their ligands, and signaling properties.... Reference Rq(R165L) Human M3 muscarinic Arrestin-2/-3 CNO* Increase Arrestin translocation Arrestin signalling...hM1Dq hM5Dq Human M3 muscarinic G αq CNO* Increase Ca 2+ Neuronal burst firing Armbruster et al., 2007 rM3D...channels, their effect, and outcome in neurons. Table 2: Descriptions of PSAMs and their activity in neurons...FRET-modified bioluminescence. Neurophotonics 11 (2), 021005. https://doi.org/10.1117/1.NPh.11.2.021005...improved coupling efficiencies. Neurophotonics, 11 (2), 024208. https://doi.org/10.1117/1.NPh.11.2.024208...Slaviero, A. N., Silvagnoli, A. D., Crespo, E. L., Schalau, R., Gott, M., Tree, M. O., Dunbar, G. L., Rossignol...

  15. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...lack thereof): Information sharing gets more challenging the bigger an organization gets, and creating...

  16. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ... iRFP713, % Oligomeric state Photo-stabilityc, t1/2, s pKa Brightness in HeLa cells vs. iRFP713, % d ...NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps are the best ...

  17. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation... revolutionized allelic exchange. Even now, only half our lab has switched over to pTOX. However, we feel...

  19. A Control for All Seasons

    Type
    Blog Post
    ...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...possible due to a lack of available materials, lethality of knockdown, or other factors, consider using...

  20. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...myriad cell types and subtypes, and a familiar challenge for anyone who has done histology on brain tissue...

  21. Plan Your Experiment

    Type
    Guide
    ...components (Figure 2), and the optimal system will likely depend on your cell type. Figure 2: Different delivery...either (1) a lack of gRNA and/or Cas9 expression or (2) a lack of efficient target cleavage in cells that...CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech.2017.11.006...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...

  22. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...ask new and interesting questions in your own lab. 2. Seek multiple mentors during your postdoc At the ...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...

  23. Molecular Biology Reference

    Type
    Guide
    ...unique restriction enzyme recognition site (Figure 2). These elements allow for the propagation of the ...elements are summarized in the table below. Figure 2: General structure of a plasmid. Plasmid Element Description...site for the next nucleotide (denoted as ddNTPs), halting synthesis after their incorporation. Sanger sequencing...converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does not require...
Showing: 281 - 303 of 303 results