We narrowed to 437 results for: ERG

1. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ...Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor supports...

2. Negative Can Be Positive: Open AAV Data with Addgene

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   ...serotypes (AAV1, 8, 9) injected into the A2 noradrenergic area of the caudal NST. (A-C) Low-magnification...

3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   ...resistance in A375 cells. The SAM system (CRISPR/Cas9 Synergistic Activation Mediator) uses an improved activator...

4. Plasmids 101: Secondary Nanobody Toolbox

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   ...PubMed Central PMCID: PMC4755751   3. Muyldermans, Serge. “Nanobodies: natural single-domain antibodies.”...

5. DNA Purification Without a Kit

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   ...broken out of cells with a solution of salts, detergent, and ball bearings. Then a cellulose dipstick ...

6. Droplet Digital PCR for AAV Quantitation

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   ...evaluation in challenged monkeys and TB diagnosis." Emerging microbes & infections 7.1 (2018): 1-9. PubMed ...

7. CRISPR 101: Making a Knock-In Cell Line

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   ...sufficient nutrients in their media, they will not undergo DNA replication, so make sure you are keeping an...

8. Lentiviral Vector Uses and Overview

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   ...Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas...

9. CRISPR Antimicrobials

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   ...introduction of antibiotics, but the field is currently undergoing a renaissance. Yosef et al. designed a two-phage...

10. Tips for Screening with Yeast Two Hybrid Systems

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    ...PMID: 11403293. 6. Chen, Y.C., Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast...

11. Plasmids 101: Protein tags

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    ... cell’s best interest to overexpress a protein. Energy and cellular resources are being spent to make ...

12. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    ...of their natural ability to infect cells, have emerged as promising tools for delivering these reagents...

13. QC Sequencing Technologies at Addgene

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    ...deposited materials. The QC team stays informed of emerging technologies in sequencing and bioinformatic analysis...

14. Bioinformatics at Addgene

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    ... which we specified as input to the subsequent bbmerge process.     Fig. 3: How we get bbdone ...

15. Antibodies 101: Immunoprecipitation

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    ... a non-denaturing buffer containing non-ionic detergents, such as NP-40 or triton-X, to preserve native...

16. Rett Syndrome: A History of Research and Therapeutic Outlooks

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    ....1206593 Powers S, Miranda C, Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein N, Meyer KC, Solano...

17. To Codon Optimize or Not: That is the Question

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    ...efficiency is determined by both codon bias and folding energy. Proc Natl Acad Sci USA 107:3645–3650 https://doi.org...

18. Hot Plasmids - August 2020

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    ...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-...

19. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    .... For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted by a cytidine...

20. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    ...difference between these two mice might drive their divergent phenotypes. Two labs, the Deverman lab at the ...

21. Designing Your Chalk Talk for the Academic Job Interview

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    ... will work. Stabilize your blood sugar. Find an energy bar that you like and consider eating one before...

22. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    ...Central PMCID: PMC384129. Aizenman, Einat, Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal...

23. Plasmids 101: Codon usage bias

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    ...efficiency is determined by both codon bias and folding energy." Proceedings of the National Academy of Sciences...

24. How to Be an Excellent Trainee

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    ...either been there or are going to be there – undergraduate students, rotation students, joining a new lab...

25. Viral Vectors 101: Preparing Pooled Libraries

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    ...cpmb.8. PMID: 27366891. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana...

26. How to Write a Scientific Review Article

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    ...ecology, this can be represented by the tip of the iceberg. For writers, this is probably “a normal day.” ...

27. Antibiotic Resistance: An Old Solution but a New Problem

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    ...clinical laboratory. What can we do to stop the emergence of antibiotic resistance?  The problem with this...

28. Plasmids 101: Stringent Regulation of Replication

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    ...Grimwade et al., 2018). Rather than serving as an energy source, ATP binding is one more way for the cell...

29. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric...

30. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ...of genome editing tools.   Jieze Zhang is an undergraduate student studying biochemistry at the University...

31. CRISPR-mediated Plant Base Editors

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    ...Central PMCID: PMC5726555. 4. Xie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex...

32. Using Video to Share Your Science: We Share Science

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    ... research, Scott Spicer concludes that “… the emergence of the video abstract genre offers a low-barrier...

33. Changing Labor Laws Bring Increased Postdoc Wages

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    ...teaching roles (such as adjuncts) and graduate and undergraduate students are exempted from the law. However ...

34. CRISPR Activation: A Practical Guide

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    ... J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F (2014) Genome-scale...

35. Tips for CRISPR Gene Editing in Mice

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    ...unknown, but fret not! The key to saving time and energy during your in vivo work is to screen you gRNA ...

36. The Challenges of Cell Culture

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    ... of tracking how many passages the cells have undergone and when to throw them away and thaw a new batch...

37. Getting the Most from Your Lentiviral Transduction

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    ...confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence, the resting phase...

38. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...the resident proteins within these compartments undergo significant post-translational modifications including...

39. Plasmids 101: Control Plasmids

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    ...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

40. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...likely won’t be long before other data repositories emerge that allow efficient searching and extraction of...

41. Plasmids 101: The Promoter Region – Let's Go!

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    ..., 10 General expression mRNA Yeast adjacent, divergently transcribed promoters Inducible with galactose...

42. The Advances Behind The World's First Personalized CRISPR Treatment

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    ...even further. In the meantime, base editing will undergo even more improvements in the lab. We’re so happy...

43. Plasmids 101: Expressing shRNAs for RNA Interference

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    .... https://doi.org/10.1007/978-1-60761-657-3_10 Vergani-Junior, C. A., Tonon-Da-Silva, G., Inan, M. D.,...

44. Hot Plasmids: Summer 2024

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    ...than using more complex protective methods or detergents like CHAPSO, while requiring a lower concentration...

45. Five Popular Model Organisms, Part 2

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    ...blog post to see even more genetic systems for emerging model organisms!) Escherichia coli One of the ...

46. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ... A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through...

47. Fluorescent Proteins 101: When GFP lets you down

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    ...protein. This is clearly the case when proteins undergo post-translational modification with fatty acyl...

48. New Optogenetic Tools for Cytoskeleton and Membrane Control

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    ...-SspB heterodimerizes under blue light BcLOV4 undergoes a conformational change and binds the plasma membrane...

49. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...21561674. PubMed Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient...

50. Plasmids 101: Biotinylation

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    ...gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the general process...