Skip to main content
Addgene
Showing: 351 - 400 of 721 results

  1. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...follow their passions, be happy, and match their personal goals to their career choices.” She points out...hard work, collaboration, and staying honest with personal goals. Stay tuned for the follow up blogs on "...

  2. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins... higher resolution images in vivo. “One of the reasons we can’t localize one cell at a depth of one centimeter...

  3. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... with multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New ...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

  4. An Addgene Summer Internship

    Type
    Blog Post
    ...Covid gene, a toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp ...biochemistry in college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan...

  5. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ... we love camelids too, though not just for the reasons the UN describes. In honor of the Camelid family...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...

  7. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...monitored genes and mutants - viral integration, transposon, and many more! Check out Addgene’s catalog to...resources on the Addgene blog Plasmids 101: Using Transposons in the Lab Plasmids 101: Screens vs. Selections...

  12. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... and then applying this mixture to your tissue (Tuson, et al., 1990; Goodpaster, et al., 2013). One issue...983. https://doi.org/10.1177/002215549804600813  Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical...

  13. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...thought about how X works?”, or even by introducing a person that the audience can relate to. It doesn’t need...  Rachel Leeson...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...communication (it is, after all, how one moves from layperson to expert) and peers can and do consume science...  Rachel Leeson...

  21. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab...this post, Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio...

  22. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...multitude of methods including surface plasmon resonance (Biacore, GE Life Sciences) or bio-layer interferometry...protein on blots with Streptavidin-phosphatase (Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). ...

  24. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...founding and running miLab to describe some key lessons I learned while becoming a health science entrepreneur...cold email Outside of the potential contacts your personal network can offer, you are really on your own ...

  25. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...from  science-focused to business-focused?" The reason is that I knew that the mind-set of the employer...instead I thought they would focus on “Has this person been consistent throughout their career?”, "Have...

  26. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...Just getting together and chatting with another person about their career path is a great start to a mentoring... read before your meetings and discuss these in person. See the extensive resource list available from...

  27. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...treatment. These responses vary depending on the person. However, in silico and in vitro assessment of ..., M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, M. P., Herskovitz, J., Goletz...

  29. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...Taking Care of Your Behavioral Health. Work on personal development Take the myIDP (individual development...a challenging period for everyone for numerous reasons, and you shouldn’t expect yourself to be as productive...

  30. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...higher Cas9-independent off-target editing in comparison with single-site editing. Encouraged by the results...#186715) and hCtRNA_VT (#186716).   Fig. 2: Comparison of previous representative dual-gRNA architectures...

  33. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    .... The problem is…how can you make a “hands-on” lesson on genetic manipulation of human stem cells when...clearer ideas about gene therapy in the following lessons. And let me tell you more, they had brilliant ideas...

  34. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...and some home-made equipment. It was humble in comparison to the labs you’d find in Kendall square, but...at BosLab Not all of our members start out with personal projects in mind. Thus, to encourage more involvement...

  35. How to Lead a Great Meeting

    Type
    Blog Post
    ...Project Meetings: You do not have to include every person who could potentially be impacted by a project....team meetings, provide agenda sections for each person to add a bullet or two about what they are working...

  36. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...." She also recommends to seek out advice from seasoned PIs who can offer support. “It is an exciting ...Email. Nothing prepared me for what a PI without a personal assistant has to deal with. My inbox currently...

  38. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...isn’t always the winner!).   Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted...

  39. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...For safety and production reasons, multiple plasmids with many components are used to package lab grown...will discuss them together. Gene expression The reason for most viral experiments is to deliver a genetic...

  45. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays...

  47. Addgene: The First Twenty Years

    Type
    Blog Post
    ...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson...

  48. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison between a full IgG antibody and common antigen...

  50. The time and cost required to make a plasmid

    Type
    Blog Post
    ...written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make...if budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’...
Showing: 351 - 400 of 721 results