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We narrowed to 398 results for: AGA

Showing: 141 - 160 of 398 results
  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...giving you a plethora of opportunities to explore! Again, attending one of these fairs is a great start, ...sure to get that person’s contact information and again, make a personal connection! Even if you don’t get...
  2. Plasmids 101: Stringent Regulation of Replication

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    Blog Post
    ...chromosomal replication and balance population growth against sustainability. Plasmid DNA replication and regulation...proteins. In nature, such plasmids ensure their propagation by limiting their copy number and compensating...
  3. Hot Plasmids Spring 2024

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    Blog Post
    ...-LV [+M], negative control) were immunoblotted against Anti-SARS-CoV-2 Nucleocapsid Protein antibodies...middle panel). Samples were also immunoblotted against vinculin as a loading control (right panel). Image...
  4. The Challenges of Cell Culture

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    Blog Post
    ...Virologists too have benefited from the ability to propagate viruses in cell culture; if there were no cells...line consisting of records of the origin and propagation of the line, and can include assays for authentication...
  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...PCR machines/Heat blocks, Lateral flow strips, Agarose gel equipment, Fluorescent emission reader Approach.... They also tried to confirm target binding on agarose gels where a cleavage reaction upon proper binding...
  6. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...2015) or the UnaG protein that binds bilirubin (Kumagai, 2013). Next to these naturally occurring co-factor...biology 27 (2015): 39-45. PubMed PMID: 26087123. 4. Kumagai, Akiko, et al. "A bilirubin-inducible fluorescent...
  7. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post
    ...visualize their target. These antibodies are generated against a specific antigen and then conjugated with a fluorescent...that you will be able to image them in the end. Again, the methods you use will depend on target and sample...
  8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ...would make it easier for an “escape” virus to propagate and infect adjacent cells. It will be important...Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. CRISPR...
  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post
    .../10.1089/hum.2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong, A. C. H., Gokal, D., Feng...Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M., Chapman, M. S., & Carette, J. E. (2016...
  10. A Control for All Seasons

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    Blog Post
    ... experimental (?) groups were run on a western against a protein standard (P) to check for tau molecular...antibody specificity is to test the primary antibody against wild-type and knockout samples. In this test, wild-type...
  11. Viral Vectors 101: Optogenetic Tools

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    Blog Post
    ...light-driven pumps that pass ions (Cl-, H+, Na+) against the concentration gradient and hyperpolarize the...activation inhibitory (ArCH) proton pump passing ions against the concentration gradient. Created with BioRender.com...
  12. Viral Vectors 101: AAV Variables That Matter

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    Blog Post
    ....2009.169 Dudek, A. M., Pillay, S., Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & ...Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator Correlates...
  13. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post
    ... Remove air bubbles between the membrane and the agar using forceps. Several protein induction conditions...detect bound cells, simply lay each membrane on an agar plate selective for your target organism and incubate...
  14. Plasmids 101: Restriction Cloning

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    Blog Post
    ...voltage difference across a gel matrix (usually agarose) to pull your negatively charged DNA through the...enzymes you used for cloning. Run your digest on an agarose gel. You should see two bands, one the size of ...
  15. 27 Hot Plasmids from 2016

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    Blog Post
    ... to screen the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully...every plasmid or as individual plasmids shipped as agar stabs. Iverson, et al. ACS Synth Bio. 2015. PubMed...sgRNA expression vectors (containing ten guides against each of the 8,158 predicted T. gondii protein-coding...
  16. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...signal in these assays (Dempster et al., 2019). Again, the experimental strategy is clear: for any gene...0059-1 Veres A, Gosis BS, Ding Q, Collins R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, ...
  17. Technologies Enabled by NanoLuc® Luciferase

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    Blog Post
    ...and Red (ReNL; plasmid #85203) NLuc fusions. The Nagai lab has additionally deposited more than 25 fusions...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...
Showing: 141 - 160 of 398 results