We narrowed to 204 results for: Arch-GFP

...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...that retain their individual activities (1). Researchers also discovered the modular format of some transcriptional...

... a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP can mediate cleavage...genes that fell into 3 categories based on the architecture of the CRISPR protein in question: C2c1, C2c2...

...first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian cells. You should also...Mammalian TAG Peter Schultz 50831 pAcBac2.tR4-OMeYRS/GFP* tyrosyl-tRNA synthetase E. coli various unnatural...or DiZHSeC Mammalian TAG P. Chen 92047 pCOTS-pyl-GFP(35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta...analogs Mammalian TAG Huiwang Ai 160041 pRaGE Pyl TAG GFP Y35TAG PylRS M. mazei Bacterial TAG Lital Alfonta... Nanxi Wang 240124 pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP PylRS M. barkeri Azidolysine (AzK) Mammalian, AAV...Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed...C321.Ub-UAG-sfGFP all TAG sites changes to UAG, RF1 function removed, with Ubiquitin-UAG-sfGFP reporter ...

...Root 105533 pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson...phGluc Gaussia EF1α Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher...extracellular pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin), a pH-stable OFP (Antares...Luciferase blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which ...or as a reference level of expression. Browse or search for popular plasmids expressing luciferase. We ...need a luciferase reporter for? Browse or use the search box to find a construct that contains your regulatory... with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc...

... includes things like viral vectors that deliver GFP.   Figure 3: A) Cumulative AAV preps available...array of ready-to-use viral vectors available to researchers around the world. While we still offer many resources...vectors from the Addgene repository can save researchers significant time and removes a variable from ...2023, we shared 93,491 viral vector preps with researchers in 45 different countries — and as of 2024, we...Addgene catalog would be broadly useful to the research community. It does, however, necessitate us first...the Data Hub before selecting an AAV can help a researcher make a more informed choice on which AAV to request...developed through the GENIE project at HHMI’s Janelia Research Campus on the GENIE website.   Bibliography  ...

...markers were not increased compared to a control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate... efficiency than its wild-type counterpart.  In search of a smaller, but equally potent Cas9 protein, ...

...aptamers able to bind to a small molecule derivative of GFP called 3,5-difluoro-4-hydroxybenzylidene imidazolinone... instead activates Spinach fluorescence. In the search for something more stable for quantitative imaging...

...(dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable DNA ...CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for CRISPR plasmids designed for...

...like Myc, GFP, and more. Neuroscience : Antibodies targeting proteins for neuroscience research. Institute...Addgene values transparency and accessibility in research. For each antibody, we provide important details...their use, visit our Antibody Guide . Browse or search the table below or visit the following pages to...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) MARCH 2016Enhancer activity... much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore... states. Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed... library     CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate...

...activated HIV-1, measured via HIV-1 promoter-driven GFP expression. In cell lines that can produce HIV-1 ...latently infected cells. To truly cure HIV-1, researchers need to vanquish these hidden viral reservoirs...“shock and kill” the HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC...could permit some viral protein production, so researchers are also interested in precisely excising the... were used for proof-of-concept studies, but researchers believe that using CRISPR/Cas9 could improve ...represent exciting advances in preclinical HIV-1 research that should be pursued in animal models, and it...benefit many others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo...

...are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...mice under a variety of behaviors, opening up new research directions that were inaccessible with earlier...hope these tools will be useful in your future research, and we’re looking forward to even more innovation...

...-2.1 construct can express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ...systems. Interested in applying Brainbow to your research? Check out Brainbow plasmids available from Addgene...

... Use the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP, or IP with Flag...Collection . Express EGFP-tagged wt and mt tau . (Hoover et al. Neuron. 2010) Express EGFP-tagged wt and mt...materials useful for neurodegeneration research. Neurodegeneration... Neurodegeneration Research Collection Addgene has assembled...collections that may help with your neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools ...studies (GWAS) or other large scale studies. Researchers are now investigating the role that these additional...additional genes may play in Alzheimer's disease. Research Tools Neurodegeneration Plasmid Collection A collection...

...vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (...Lab . Cas-Designer (Link opens in a new window) Searches for targets that maximize knockout efficiency ...Signaling and Functional Genomics at the German Cancer Research Center (DKFZ) in Heidelberg. CRISPR FAQ forum ...Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles...plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification...packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Pooled libraries are also available for human and...

...fluorescent protein that could be co-imaged with GFP using the same wavelength for excitation. The result...statement: Accelerate research and discovery by improving access to useful research materials and information...post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were...examples of technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather... or above 600 nm wavelengths should be used. Researchers often use bioluminescence to overcome these issues...brightness of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however...leads to spurious activation of the FRET sensor. Researchers have sought to alleviate many of these challenges...

...apart. While the classic Green Fluorescent Protein (GFP) and other fluorescent proteins revolutionized live-cell...localization. HiBiT-tagged targets in translational research Researchers can incorporate HiBiT with a wide range ...with knock-in models, ectopic models provide researchers with a means to quickly iterate, work across ...use of HiBiT provides a means to quickly answer research questions before creating knock-ins.  ... Cracking the code of 'undruggable' targets Researchers can design drugs to bind to well-defined ligand-binding...CRISPR knock-in HiBiT-tagged EGFR cells allowed researchers to track endogenous activity of EGFR, including...advances detection of endogenous proteins As researchers continue to improve more physiologically and ...

...Church lab uses this system to control expression of GFP.)  To turn this inducible system into a repressible...the complexity of the system, researchers have used split GAL4 architecture or combined the system with ... ON state, and how you might use these in your research. Today, we’ll look at repressible promoters, which...bind the promoter and repress pADH1 activity. Researchers have engineered pADH1 variants that are less ...

...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...developed by the GENIE Project team at HHMI's Janelia Research Campus (Aggarwal et al., 2025).In this latest ...genetic cargo of choice. After hearing about researchers' favorite features and limitations of existing...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here... packaging and strong expression after inserting EGFP and provide step-by-step protocols for viral generation...Figure 2). Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set...

...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors (or...adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV) Modified form...Wild-type adenoviruses have been modified by researchers to create recombinant adenoviral (rAdV) vectors...gene expression patterns that were observed, researchers have tended to favor using first- and third-generation...is still the most commonly used generation for research purposes. The system consists of two plasmids ...