...transcriptase are packaged (Wu et al., 1997) inside of the capsid . This is useful for viral reproduction, and for...for reverse transcriptase (RT) is present in the capsid, as the engineered, recombinant genome is (thankfully...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

...DNA transposons are ubiquitous genetic elements capable of spreading within and between genomes, and have...transposition. By natively combining the DNA integration capabilities of transposases and the target programmability...large DNA cargos into bacterial genomes. This new capability was named INTEGRATE (Insertion of Transposable...

...AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this approach has been...separate AAV vectors, increasing overall packaging capacity but necessitating purification and co-infection...-AAV toolbox that retains the gene-targeting capabilities of full-length SpCas9. This set of plasmids ...

...that combines enrichment and imaging of proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM...GFP fusion in this proof-of-concept study) are captured on streptavidin-coated magnetic beads through ...bundle; SAH: 60-nm single alpha helix) and target capture module (SpyTag-GFP nanobody) assembled on beads...

...interest. Although they have a small packaging capacity, AAV vectors were suitable for our needs for the...Joshua C., and Richard J. Samulski. "Packaging capacity of adeno-associated virus serotypes: impact of...recombinant adeno-associated viral vectors: packaging capacity and the role of rep proteins in vector purity....

...of interest as well as sequences necessary to encapsulate that insert into a viral particle (the packaging...cell culture as lentivirus-derived vectors are capable of transducing certain quiescent or terminally ...ELISA dosing method detects the expression of viral capsid antigen p24, the FACS dosing method correlates ...

...sheath fluid and transported through tubes or capillaries to a laser. As the cells move through the fluidics...different directions. The degree of scatter is captured by detectors and provides useful information about...fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of the emitted photons...

..., brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques in biotechnology...Fluor HaloTag Ligands expand the self-labeling capabilities of this platform. The Janelia Fluor ligands .../doi.org/10.1007/s41048-017-0037-8 Lucidi, M., Capecchi, G., Visaggio, D., Gasperi, T., Parisi, M., Cincotti...

...entire field of view is excited at one time and captured in a single camera exposure. This is much faster...out-of-focus light. The most difficult characteristic to capture in these types of samples is their fast movements...imaging, the light microscope was limited in its capacity to resolve closely lying structures. This is because...

...to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate plasmid yield...ensure proper lysis, but you will still face column capacity limitations since the columns can be “clogged”...magnet as you scale up. Table 1: Standard Prep Kit Capacities* Prep size Amount of culture Approximate ...

...Cas9 fused to an engineered adenosine deaminase capable of chemically converting adenosine (A) to inosine.... They also developed a machine learning model capable of predicting editing outcomes of their various..., they added the pioneer factor SOX2, which is capable of binding to and remodeling inaccessible chromatin...

...Ventures like Impact Story seek to find ways to capture scholarly work that exists outside of traditional...Of course, no one measure will ever adequately capture a notion as complex as “scientific impact.” As ...

...It’s pretty hard to escape news and fears of Ebola these days, as the infectious disease once limited...described how the glycoprotein transforms itself to escape vesicles within host cells and turn the cell into...

...2019 solidified the project and the findings were captured in this just released report “Enhancing Dissemination...of priorities for pd|hub which include building capacity to identify the most effective practices and disseminating...

...scientific gains by adding long-read sequencing capabilities to complement our next-generation sequencing...flourishes when data are shared. The scientific landscape is evolving rapidly, and with it, the needs of...

...described in Nature Communications last year, “The capability to control spectral properties of PA FPs with...is also looking in the direction of biosensors capable of revealing, for example, how muscles work inside...

...gnome. See this video where he/she/it/something is captured on camera: Unfortunately, unless they’ve worked...were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to be discovered...

...sheering of DNA by sonification. Though it helps capture weaker or more transient DNA-protein interactions...fusion proteins Study chromatin interactions with CAPTURE Additional Resources on Addgene.org Find simple...

.... Importantly, metadata for each screen is captured by BioGRID curators, including the cell line/tissue...determine the gene hits for each screen are also captured as metadata.   Screen results are reported as ...

.... We don’t raise money except occasionally for capital equipment or resources needed to launch a new service...workforce for when we need to be working at full capacity to serve the scientific community. First, we applied...