We narrowed to 191 results for: CO

1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   Blog Post

   ... BiFC constructs. If your PIPs actually interact, co-expressing one of the endogenous PIPs should decrease...

2. Antibodies 101: Immunoprecipitation

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   Blog Post

   ...linkage. This means that the primary antibody will not co-elute with the target protein, eliminating the risk...

3. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...barriers to implementation. Funded by the NSF (with Co-PIs Jessica Bell, Lisa Gentile, Ellis Bell, and Amy...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I ...

5. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone. ...

6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   Blog Post

   ...University of North Carolina at Chapel Hill. Under the co-mentorship Blossom Damania and Jack Griffith, her...

7. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ...work and make this happen. Be realistic and: Your co-workers will learn to work within these bounds You...

8. CRISPR Plasmids - Tagging

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   Collection

   ...protocol, generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled...

9. Fluorescence Titering Assay

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   Protocol

   ...pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container...

10. Tips for CRISPR Gene Editing in Mice

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    Blog Post

    ...BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330 plasmids containing sgRNA ...

11. Overcoming the AAV Size Limitation for CRISPR Delivery

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    Blog Post

    ...packaging capacity but necessitating purification and co-infection of two AAVs. Cas9 orthologs: shorter, ...

12. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    ... General Meeting (now Microbe) in 2013 in Denver, CO. While I had been to the General Meeting seven other...

13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ... activation domains to the same genetic locus via co-expression of an epitope tagged dCas9 and antibody-activator...

14. Hot Plasmids: Fall 2024

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    Blog Post

    ... fluorescence markers like GFP (510 nm), allowing co-imaging. And because bioluminescent markers do not...

15. CRISPR Plasmids - Empty gRNA Vectors

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    Collection

    ...present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid ...Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875...

16. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post

    ...type’ control can be made one of two ways. First, co-expression of a suppressor tRNA (SupD) instead of...

17. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    ... allow stronger counter-selection than one merely co-opted toward that aim (i.e. sacB). Importantly, to...

18. Five Popular Model Organisms

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    Blog Post

    ...Saccharomyces cerevisiae to human: The important gene co‑expression modules." Biomedical Reports 7.2 (2017...

19. Plasmids 101: Biotinylation

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    Blog Post

    ...biotinylated (more on this below) it can be incubated or co-expressed with avidin or a structurally similar protein...

20. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post

    ...exogenous BV and after normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16...