We narrowed to 960 results for: Cre

...Specific Recombination in Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids...

...Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo...representation of common injection routes in mice. Image created with biorender.com.   Needle techniques Intravenous... often used when injecting a cell suspension to create xenograft models. Restraint: back scruffing or ...

...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more...requirement for the HIV Tat protein, thus decreasing the probability of creating replication-competent lentivirus...an extensive collection of lentiviral plasmids created for a variety of applications including cDNA expression... very common due to its broad host range, and increased particle stability; however, the protein is cytotoxic...replication-deficient meaning they can be used to create virus that is capable of infecting target cells...our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr...

...used for large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert...GAT; increased nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease...others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...backbone HypaCas9 — increase Cas9 proofreading and discrimination evoCas9 — decrease off-target effects...domains to expand PAM recognition, increase specificity, and decrease off-target effects Sniper-Cas9 — ...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 — increases fidelity with reduced nuclease...integration of donor DNA. Genome-Wide Screens Using CRISPR In a genetic screen, a population of cells with mutations...

...PMCID: PMC5529245. Deverman, Benjamin E., et al. "Cre-dependent selection yields AAV variants for widespread...determined LY6A was a receptor used by PHP.B viruses. Creating PHP.B, a neurotropic AAV9 variant PHP.B’s specificity...the Gradinaru labs engineered PHP.B. PHP.B was created by first generating a diverse library of AAV9 mutants... the AAV9 capsid. This mutant library was then screened in vivo in C57BL/6J mice strains to identify variants...injection. Compared to AAV9, PHP.B has a >40-fold increase in CNS transduction and transduces the majority...Ly6a in primary C57BL/6J brain endothelial cells decreased transduction rates while overexpression of the...

... approach proved a challenge. The old standby of CRE/flp didn’t work – it turns out these tyrosine recombinases...likely do it using Prime editing alongside some creative (and significant!) protein engineering. They combined...insert being “dropped” in the wrong place, while increasing the number of potential applications. But they...

... The authors have deposited AAV plasmids for Cre-dependent and -independent versions that are trafficked...O’Neill The Lin Lab and collaborators recently created ASAP5, a faster and more responsive genetically-encoded...for its improved kinetics and responsivity in screening ASAP3 variants. B) Electrical and optical recordings...

... mutant allele of a gene (point mutant)? Increase or decrease expression of a target gene? Once you have...High-fidelity Cas enzymes increase specificity Dual-nickase approach increases specificity but is less ...efficiency. In fact, cleavage efficiency may increase or decrease depending upon the specific nucleotides ... the general framework of a CRISPR experiment. Created with BioRender.com. Select Your Desired Genetic...mammalian cell lines Interfere Activate (CRISPRa) Increase gene expression without permanently modifying ...likely won’t be able to completely avoid them. To increase specificity, consider using an engineered Cas ...target in your samples to ensure there are no discrepancies with the reference sequence you used for gRNA...

...Upon activation, NK cells secrete perforin and granzyme B, two enzymes that create pores within an infected...LSIGN, MGC129964, MGC47866 CREB1 cAMP responsive element binding protein 1 CREB, MGC9284 CTSB cathepsin ...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like... SDS, SWDS SCG2 secretogranin II (chromogranin C) CHGC, SN, SgII SCGB3A1 secretoglobin, family 3A, member...HIN1, LU105, MGC87867, PnSP-2, UGRP2 SCT secretin - SCTR secretin receptor SR SCYE1 small inducible cytokine... B cells mediate humoral immunity through the secretion of antibodies, and also function as antigen presenting...

...with work directed towards increasing on-target editing rates and decreasing off-target editing rates. ...others. Homology-directed repair (HDR) allows us to create a variety of modifications in endogenous loci with... locus makes it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify ...sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR technology... : Base editors directly convert bases without creating a double-strand break. This method displays higher...method targets RNA rather than DNA, it does not create permanent genome edits or double-strand breaks....epigenetic modifiers can modulate transcriptions and create heritable epigenetic marks. Visualize : Fluorescently...

...reprogramming of adult pancreatic exocrine cells to beta-cells. Nature. 2008 Aug 27. Melton Pancreatic Acinar Cells...human cells in 2006 and 2007, respectively. To create iPSCs, fully-differentiated adult somatic cells...Plasmids in the table below. Once iPSCs have been created they can be directly differentiated into specific..., and transdifferentiation utilize plasmids to increase expression of key factors. The tables below will...plasmids available from Addgene that can be used to create iPSCs or another cell type. You can also search...iNDI) Collection for plasmids that can be used to create cell lines with endogenously-tagged gene variants...Plasmids Browse the table below for plasmids used to create induced pluripotent stem cells (iPSCs). Sort the...

...double stranded breaks. Cytosine base editors are created by fusing Cas9 nickase or catalytically inactive...converted to thymidine through base excision repair, creating a C to T change (or a G to A on the opposite strand..., which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine DNA deaminases...deaminases do not exist in nature, but have been created by directed evolution of the Escherichia coli TadA...evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both types of base editors...adjust the editing window, or adding fusions that increase product purity such as the DNA glycosylase inhibitor... to the PAM sequence, some base editing systems create a wide spectrum of single-nucleotide variants (...

... cell cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized...immortalized B cells). The genetic reorganization that creates variety in the antibody allowing for their extreme...animals immunized as described above are used to create hybridomas, which produce large amounts of a single...recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma...goes through a randomized reorganization process, creating a unique binding site. This process, known as ...roles in the immune response, and can be used to increase flexibility or specificity in antibody-based methods... Valency Subclasses IgA Prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for the Innovative Genomics Institute...), including plasmids for protein production, secretion, and codon-optimized versions designed...metalloprotease that cleaves hACE2, potentially increasing viral infectivity (Heurich et al., 2014). PCP4...-CoV-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain...Binding Domain Deep Mutation Scanning Library Screening Jesse Bloom Library of Spike protein RBD mutations... Domain Site-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries of variant Spike...Beating Affected by the Pcp4 Dose-Dependent [Ca2+]i Increase in Down Syndrome Mice, Ts1Rhr . International ...

...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...common epitope tags, as well as antibody plasmids to create or express plasmid-based antibodies. Most antibodies...functional neural circuits at the growth cone. Image credit: IPI Browse Netrin, DCC, and IGDCC3 (PUNC) Antibodies...postsynaptic membranes at neuronal synapses. Image credit: IPI Browse Neurexin and Neuroligin Antibodies ...function, and/or detect integrin expression. Image credit: IPI Browse Integrin Antibodies ID Recombinant ... Plasmids The following plasmids can be used to create or express plasmid-based antibodies. ID Plasmid...

...floxed neoR gene is removed via infection with adeno-cre. AAV-based gene editing compared to CRISPR editing... repair template. It is this application - the creation of sequence-specific knockins - for which the ...Asuri et al, for example, used zinc fingers to increase the efficiency of AAV mediated genome editing ... and encourage to deposit any new AAV tools you create. Many thanks to our guest blogger, Todd Waldman...

...antibiotic resistance markers, and other enzymes such as Cre-recombinase drastically expanding the base-editing...Base editors create specific point mutations in the genome, but they’re inefficient compared to CRISPR...experiments. In the past few years, scientists have created an array of base editing reporters that can help...Cas9-mediated editing in real time The Harris lab created ACE, a reporter that monitors APOBEC (a family ...and a downstream constitutively active eGFP. To create the inactive mCherry, the lab introduced a 43 base-pair...DSB in their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target...complexes in real-time (St. Martin et al., 2019). To create the eGFP reporters the Harris lab individually ...

...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...smaller fragment of an antibody, which could offer increased stability and ease of production due to their ...highlights plasmid collections that can be used to create and express plasmid-based antibodies. Description...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...

...Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's Disease.... How to Order Creating an Account: Before placing your order, you will need to create an account and ...for cloning and assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors... our website. Placing an Order: After you have created an account, you can place orders through our website...

...factor isoform in pooled screens. This collection and library can be used for screening transcription factors...available at Addgene. The collection can be used for screening transcription factors for cellular programming...window) . TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X...bp sequence, to simplify identification during screening. Library ID PI Description MORF library 137000...