We narrowed to 430 results for: ERG
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TypeCollection...here . pAra Series Various Plasmids pBAD GFP Bryan Berger Reporter system for testing hetero- and homo-dimer...
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Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...need, we're incredibly grateful that the Chan Zuckerberg Initiative was able to step in to be our “port...laboratories all around the world." We thank the Chan Zuckerberg Initiative for supporting Addgene by providing... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...proteoglycans on the surface of the packaging cells (Vandenberghe, 2010). Rather than being released into the ...22915587. PubMed Central PMCID: PMC3476254. 7. Vandenberghe, Luk H., et al. "Efficient serotype-dependent... -
Another Pathway into Cells: iTOP
TypeBlog Post... Subsequent work found that both NaCl and non-detergent sulfobetaine 201 (NDSB-201) were required for ... PubMed. Is VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8)... -
K. phaffii: Rising to the Occasion
TypeBlog Post...utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K. phaffii is....2017.941 Bernauer L., et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...transition your undergraduate students from the classroom to the lab. You could give your undergrads hands-on... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...LabCentral is a lab and office space shared by numerous emerging biotech companies. The space was modern with clean...When I heard about how LabCentral aims to enable emerging biotech companies, I was excited to go there because... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...human U2OS cells. Based upon this, they chose an intergenic DNA region (IDR3) as a reference loci to measure...measure inter-locus distances of loci pairs from intergenic regions, intronic regions, and a pericentromeric... -
How to Lead a Great Meeting
TypeBlog Post...useful information, and invigoration - a renewed energy for continuing the project after leaving the meeting...you will be running collaborative, informative, energizing meetings all your own. Additional Resources... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...had some supervisory experience working with undergraduate students? On top of that, perhaps you were involved...career services offices. You might think only undergraduates use these services, but some of the general... -
Luciferase Plasmid Collection
TypeCollection...complementation in plants. Bioluminescence Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular ... -
Antibodies 101: Isotypes
TypeBlog Post...protect against parasites but also play a role in allergies. They are found bound to IgE receptors on the ...cells, two types of white blood cells involved in allergic reactions. There are no IgE subclasses and they... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post... luciferase substrate, the luciferase transfers energy to the FP, resulting in a fluorescent signal. Since...based on the principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...SspB) that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...control plasmid for expression in forebrain GABA-ergic interneurons (83895). See our Serotype Testing AAV...which targets expression of ChR2 to forebrain GAB-ergic interneurons through the mDlx enhancer element. ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...this year with a fresh team of twenty excited undergrads. Team IISER-B’s idea for the iGEM 2019 is to ...Bhopal which received the Silver Medal in the Undergrad category at the iGEM Competition 2019. He has ... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post... either. Coming from a strong background of undergraduate research straight to a lab career at a biotech...more! This role is a good fit for you if you are energized from interacting with others, and have strong ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post... products are then transformed into E. coli to undergo nick repair. This method can work well, but poor... starts out in familiar territory, but quickly diverges from previously discussed methods. As described... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post... Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill...j.molcel.2014.08.002 Ran FA, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...learned along the way. Own your time When I was an undergrad, I was assigned a paper to give as a journal club... of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget a point...