We narrowed to 877 results for: HA

...CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified...which one of Cas9’s two critical enzymatic residues has been converted to an alanine (D10A or H840A), changing... for your experiment, ensure your sgRNA sequence has little homology to other regions of the genome and...looser PAM requirements compared to SpCas9. xCas9 has a PAM requirement of NG, GAA, or GAT, making it a...SpG variant, discovered by the Kleinstiver group has a PAM of NGN (Walton et al., 2020). Further, this...reverse transcriptase, and a nCas9. While this system has been shown to be less efficient than base editing...directly fusing the cargo to dCas9.  This technique has been exploited to localize factors such as transcriptional...

...inflammation and degeneration has been reported (Muraine et al., 2020). AAV8 AAV8 has high transduction efficiency...nervous system (CNS) (Wu, Asokan, et al., 2006). AAV1 has also been found to transduce skeletal muscle, cardiac... the most common choice for AAV pseudotyping. It has broad tissue tropism, including liver, muscle, lung...neural connections. Although derived from AAV2, AAVrg has not been thoroughly studied in contexts outside the...generally the best choice for targeting astroglia. It has additionally been reported to have tropism for liver...administration (Halbert et al., 2001). It additionally has tropism for cardiac and liver cells (Issa et al.,...applications targeting liver tissue. In addition, it has been shown to transduce skeletal muscle, cardiac,...

...give me a brief history of optogenetics? How long has this idea been around? Boyden: Optogenetics is a ... silencers of neural activity. Since then, there has been a steady stream of papers describing new photosynthetic...against each other. Getting two-color stimulation has been a holy grail of the field. The problem is that...is fundamental understanding of the brain, which has humanistic and philosophical implications. As an ...Addgene over the years, many of them very popular. Why has it been so important to you to distribute these tools...as possible? Boyden: I think because neuroscience has so many different goals: to understand how a mouse...

... to be activated by the DREADD actuator after it has been administered. Although we found a larger proportion...DREADD to the plasma membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin...al., 1988). Thus, we injected AAVs carrying hM4Di-HA in the brains of mice and monkeys. Surprisingly, ... a big difference. Replacing the mCherry with the HA tag resulted in abundant expression of hM4Di in plasma... where the fluorescence is not needed, the use of HA and other short epitope tags (Kimple et al., 2013...used to reveal the hM4Di coupled to mCherry (top) or HA tag (bottom). The images show examples of dendrites... to the plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di...

...both a fundamental driver of these principles and has emerged as a byproduct of connectivity. Between 2012...integrated with most of the tools noted above, and has a modern API. Integrations with additional tools ..., so it’s possible to re-export a protocol which has been edited back to protocols.io. Thus, RSpace becomes...Figshare. Any ELN that facilitates reproducibility has to make it easy to export data from the ELN directly... these five general purpose repositories. RSpace has been integrated with two of the five: Figshare and...additional metadata in the repository after the deposit has been made. It’s possible to associate the depositor...the repository. The ability to deposit data that has already been structured in folders or notebooks in...

... a family affair back in 2004. As Addgene has grown, it has taken effort to continue the tradition and...goofing off, the company culture here at Addgene has remained paramount. Candidates who interview at Addgene...testament to the familial aspect of Addgene. As our size has drastically increased, it would have been a tight..., even for an event where there is food and beer. Ha! We had over 60 people join the fun - including a... or binging a new Netflix series. The thing that has made all the difference here at Addgene is the emphasis...and a cooler. Chances are one of your colleagues has a frisbee at least. Use it! 8. Organize If you’re...there is something fun happening! Addgene actually has chimes outside of our break room. A ring of the chimes...

...which a good antibody has not been developed (and just because your protein has a commercially available...localization, or protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent proteins,...affinity tag to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses of the ...Purification Good for antibody-based purification; has inherent enterokinase cleavage site GST Large Protein...against proteolysis, but may reduce solubililty HA YPYDVPDYA or YAYDVPDYA or YDVPDYASL  1.1 Affinity... a moment), and 2 ProtA IgG-binding domains. TAP has since come to encompass several other tag combinations...necessarily want them to stick around after that purpose has been served. At this point, proteases can be your...

...EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene #236081...by the Loren Looger and Kaspar Podgorski Labs now has a new version: iGluSnFR4, developed by the GENIE ...we're excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed...Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin resistance...Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA [12CA5] - Chimeric (Addgene #198003)) and Goat anti-Mouse... loci in the genome. The modified transposon RNA has untranslated regions (U, blue) on the outside of ...12 kb — with efficiencies between 5% and 11%. It has higher efficiency and lower indel formation than ...

...artifacts, it has better folding and maturation kinetics than other dark fluorescent proteins, it has good signal...Technology (KAIST) and Institute for Basic Science (IBS) has developed another useful optogenetic tool that enables...facilitate future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic...operon originates from Pseudomonas putida CBB5 and has been tailored for expression in E. coli. This operon... contributed by Christina Mork The Murakoshi lab has developed two new FLIM-FRET acceptors, ShadowG, a... due to its very low quantum efficiency. ShadowY has a lower quantum yield and higher absorption than ...

... world has battled the COVID-19 pandemic over the past few months, the scientific community has seen a...research into this disease. A key reason this research has been able to progress so rapidly is scientific sharing...is applicable to researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of ...COVID-19 Resources Canada site. To date, the portal has recruited 3,218 Canadian volunteers, and features...

...story, to get scientists excited about what Addgene has to offer, and to help scientists find what they need...homepage. Blugene, our trusted friend and mascot, has undergone a make-over as well. While in the course...Blugene needed the same thoughtful treatment. Blugene has been with us since 2011, and while you may have seen...and sharing.  One big feature upgrade? Blugene now has a full expression. You’ll mostly see a friendly smile...an experiment doesn’t go as planned. Blugene also has full arms so that they can hold and hug items closer...

... Even though GFP has been so thoroughly characterized, it turns out this protein has a few more secrets...photoconvert GFP from green to RED! This simple technique has been shown to work in plant, Drosophila and mammalian...cases, however, a GFP-tagged cell line or organism has already been made, and it would be expensive and ...is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh...

... even society. One obvious way to do that is and has been to publish in journals with a high “impact factor...cited articles appearing in lower-prestige journals has risen steadily from 1995 to 2013. Considering citations...online access on scientific impact Partly, the story has to do with online accessibility. It is now about ...among the most cited. And, as scientific discourse has increasingly moved online, surely times have changed...

...restriction enzyme, you will need to verify that it has be cloned in the correct orientation - this can be...

...Retroviridae family. Infection with lentiviruses has a long incubation period that eventually leads to... genes, mutations, or treatments into cells, and has been widely used in the research community for decades...VSV-G is by far the most common envelope gene, as it has a wide range of infectivity across many cell types...their stable expression and wide tropism. Addgene has an extensive collection of lentiviral CRISPR libraries...

... how a plasmid has been used by multiple labs and see what experimental systems it has been validated ...validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to the... and the PubMed ID # (PMID) for the article that has used this plasmid. Happy researching! If you need...

... genes, mutations, or treatments into cells, and has been widely used in the research community for decades...VSV-G is by far the most common envelope gene, as it has a wide range of infectivity across many cell types...promoter; the transcript begins at the beginning of R, has a 5' cap structure, and proceeds through U5 and the...

...Service since 2009. He has  developed a proprietary, low cost cloning protocol that he has used for cloning ...least 24 bp with the template and if the sequence has high GC or AT content, increase the overlapping region... most of the troubleshooting regarding this step has to be strategy specific, there are few general parameters...ratio of 1:2 (plasmid:insert), but this parameter has to be optimized according to the strategy adopted...satellite colonies and they form when all the antibiotic has been used. Don’t worry too much, just pick the big...

...   By Ashley Waldron Proximity-dependent labeling has become a powerful approach for proteomic mapping ...our 2019 overview). Until recently, the approach has been hampered by the need to tag each protein of ...M-CREATE) by: Brian O'Neill  The Viviana Gradinaru Lab has previously developed the multiplexed version of the...sequence knock-ins by: Lucie Wilson Le Cong’s lab has developed a method using dCas9-SSAP to knock-in long... methods for large inserts. However, this system has a much lower error rate. For a one kilobase insertion... its functions. Recently, Ravid Straussman’s lab has developed visual barcodes for clonal multiplexing...

...An antibody that has been cited thousands of times may be a safer bet than one that has never been used...work. Whenever possible, choose an antibody that has been validated in your specific application. Consider...cross-reacts with. Ideally you will find an antibody that has been validated in the species your protein of interest.... Talk with your peers. If someone in your field has used the antibody before you will not only have peace...Uhlen, 2016). Be sure that the antibody you choose has been validated using one or more of the proposed ...