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We narrowed to 364 results for: Pol

Showing: 141 - 160 of 364 results

  1. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...include training or teaching others, research, and policy development. Every worker at every level does better...all times when making decisions, assignments and polices. Sometimes the boss has to be the boss and make...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... bacterial genome. Cas genes, which code for polymerases, nucleases, and helicases, were also disrupted... into three distinct types and subtypes using polythetic classification based on evolutionary similarity...

  5. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  9. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...Membrane-Rupturing Microscopic Tools In this post, Jessica Polka, formerly a postdoctoral researcher in the Silver... other chemical fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH...

  10. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...HiFi so accurate (apart from the high-fidelity polymerase) is the ability to remove, reset and ligate mismatched...primer design based on fragment sequences and the polymerase used in the amplification. This tool allows instant...

  11. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...and Oskar protein were detected at the posterior pole. Oskar protein levels were negatively correlated... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that...

  13. 5 Great Apps for Lab Life

    Type
    Blog Post
    ... depending on the primer sequence and the NEB polymerase used. Overall, while much of the information ...allow you to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, ...

  14. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

  17. Antibodies 101: ChIP

    Type
    Blog Post
    ...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  19. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...shoulder for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip...organizational work. LinkedIn is not the platform for politics, family updates, or click bait of any kind. For...

  20. The time and cost required to make a plasmid

    Type
    Blog Post
    ...plasmid? Figure 2: Survey results from a Twitter poll run in 2023, n=194.   After the great Plasmid...much harder to calculate, in both our 2018 and 2023 poll, most people said it took them more than 22 hours...
Showing: 141 - 160 of 364 results