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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 181 results for: SPEN

Showing: 141 - 160 of 181 results

  2. Plasmids 101: Protein tags

    Type
    Blog Post
    ...protein. Energy and cellular resources are being spent to make something the cell doesn’t need to make....

  6. Viral Production at Addgene

    Type
    Blog Post
    ... pellets containing viral particles are then resuspended in PBS, aliquoted, frozen, and stored at -80 ...

  15. Finding Your Science Policy Path

    Type
    Blog Post
    ...academia is the uncertainty of it all. Though I had spent the first few decades of my life following a very...

  20. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...20 min. Resuspend dry DNA with TE (10 mM Tris-HCl pH 8, 0.1 mM EDTA). Pro-Tip DNA resuspension can take...culture of bacteria transformed with your plasmid Resuspension buffer Denaturing solution Renaturing solution...several tubes/bottles. Remove the supernatant and resuspend the bacteria in buffer. Note: This step gets all... all of the bacteria back into suspension, but within a smaller volume of buffer that is compatible with...next solution. Add a denaturing solution to the resuspended bacteria. Note: This step causes the bacteria...column with 70% ethanol to remove excess salt. Resuspend the DNA pellet, or elute the DNA off of the column...Prepare the following solutions: Solution I - Resuspension Buffer 25 mM Tris-HCl (pH 8) 50 mM glucose 10...
Showing: 141 - 160 of 181 results