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We narrowed to 165 results for: cas9 expression vector

Showing: 141 - 160 of 165 results

  1. CRISPR Plasmids - Purify Genomic Loci

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... for enChIP, as well as an anti-Cas9 antibody. Biotin tagging of dCas9 can be achieved by fusing a biotin...biotin acceptor site to dCas9 and co-expressing BirA biotin ligase, as seen in the CAPTURE system. The ...purify genomic loci. Plasmids are available for expression in mammalian systems, bacteria, and yeast. Mammalian...inactive dCas9 is used to purify genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or ...purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding known transcription...

  2. Neurodegeneration Research Collection

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    Collection
    ...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New...Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent rodent...species. Boender et al. Sci Adv. 2023 Jun 2. Use Cas9 in astrocytes. Endo et al Science. 2022 Nov 4. Base... dopamine research. CBA-driven, Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion...Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, ...

  3. Botman-Teusink Yeast FP Collection

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    Collection
    ...Y., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for...Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas...Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of interest with ...yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link plasmid (Sheff et al...FPs in yeast. Vectors are based on the pDRF1 plasmid (Loqué et al., 2007) containing the PMA1 promoter and...blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902. https...Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression of codon-optimized FPs ...

  4. CRISPR Plasmids - RNA Targeting

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    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each DNA target have...targeting plasmids. Plasmids are available for expression in mammalian systems, bacteria, and plants. Mammalian...

  5. CRISPR Plasmids - Parasites

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    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in parasites. CRISPR...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and forward...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...

  6. Plan Your Experiment

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    Guide
    .../or Cas9 expression or (2) a lack of efficient target cleavage in cells that do express both Cas9 and ...Figure 1). You will decide how to express Cas9, the delivery system for Cas9 and the guide RNA (gRNA), the...depositors ). Browse empty gRNA expression vectors . Select Your Expression and Delivery System Once you ...Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression levels, long-term...about viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral ...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...non-homologous end joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity...

  7. Distribution to Industry

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    Collection
    ...while also adding additional filters such as expression, vector type, species, and more. Plasmids Browse ...CRISPR Thorsten Stiewe Use these donor vector libraries for CRISPR/Cas9-mediated saturation genome editing...for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet 95 plasmids for cloning...

  8. CRISPR Guide

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    Guide
    ...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in ...a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing...strand SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...new tools. Figure 3: Overview of Cas9n Nickase and dead Cas9 The two Cas9 nuclease domains, RuvC and HNH...enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no defined...

  9. Cre-Lox and Other Site-Specific Recombinases

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    ... plasmids expressing Cre recombinase and filter the results by expression system and vector type. Find... GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression Plasmids 101: Knockout/Knock-In... plasmids expressing Flp recombinase and filter the results by expression system and vector type. Find... plasmids expressing Dre recombinase and filter the results by expression system and vector type. Find...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...

  10. Modular Cloning Guide

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    Guide
    ...Cloning System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing several...Yeast Expression, Insect Expression Chen Davidovich 41 plasmids to construct multigene expression vectors...4G-Cloning Plasmid Kit Bacterial Expression, Insect Expression, Mammalian Expression Stephan Gruber Platform for...assembly of expression vectors for multi-subunit protein complexes in various hosts. Expression cassettes...Toolkit Mammalian Expression Yizhi Cai 95 plasmids to generate mammalian expression vectors for transient ...assemble mammalian expression vectors for proximity labeling experiments. Multiplex CRISPR/Cas9 Assembly Systems...Mammalian Expression Takashi Yamamoto Plasmids for construction of all-in-one CRISPR/Cas9 vectors expressing...

  11. Hot Plasmids: Summer 2024

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    Blog Post
    ...much smaller proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed...al., 2024). In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study...showed that AAV-delivered CHARM could silence the expression of prion protein in mouse brains for at least...gene product; it simply turns the target protein expression off. Plus, DNA methylation is a long-lasting ...-silencing CHARM variants that stop their own expression after silencing their target — potentially improving...by targeting marker proteins that are uniquely expressed in a given cell type. For example, glial fibrillary...GFAP) is a glia-specific intermediate filament expressed in astrocytes throughout the brain, making it ...

  12. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    Blog Post
    ...We generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP...plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your ...enChIP system enables us to express the CRISPR complex much easier in various cells, including primary mouse... IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. Fujita T, Fujii...

  13. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post
    ... DNA compaction, an enhancer can increase the expression of a gene that is millions of base pairs away...Putting it all together, we get the following expression for step 1: (H3K4me1 + H3K27ac) – H3K4me3 The...enhancers with CRISPR Armed with the genome math expression for NANOG enhancers, we’re ready for step 2: ...of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

  14. Tetracycline Inducible Expression

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    ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter ...Church 87360 TLCV2 Lentiviral vector for tet-inducible Cas9-2A-EGFP expression. Based on LentiCRISPR v2 ....Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...

  15. Adeno-associated virus (AAV) Guide

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    Guide
    ...Viral Vectors AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements, making...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...

  16. Adenovirus Guide

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    Guide
    ...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...third-generation vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette ...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...Generations First-generation rAdV vectors The first generation of rAdV vectors was developed by removing the...

  17. Bacterial Expression Systems

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    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...systems. ID Plasmid Promoter Inducer Expression Species PI 44249 pdCas9-bacteria pTetO Anhydrotetracycline...

  18. Guide to Using Pooled Libraries

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    Guide
    ...on genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts...given library have the same backbone, but they express or target different genes. Some libraries cover...like lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen...popular libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale,...

  19. CRISPR Plasmids - Repress Gene Expression

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    ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-repressor...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...peptide like KRAB, can knock down gene expression by interfering with transcription. Design your gRNA to target...transcriptional inhibition. Plasmids are available for expression in mammalian systems, bacteria, plants, and yeast...Tools CRISPR Blog Posts Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...

  20. CRISPR Plasmids - Activate Gene Expression

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    ...also express a gRNA, you will need to use a separate gRNA expression plasmid to target the dCas9-activator...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... plasmids for activating or upregulating gene expression in various model organisms. CRISPR...transcriptional activation. Plasmids are available for expression in mammalian systems, bacteria, Drosophila, plants...Design Tools CRISPR Blog Posts Catalytically dead dCas9 fused to a transcriptional activator peptide can...specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your...
Showing: 141 - 160 of 165 results