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We narrowed to 150 results for: cas9 expression vector

Showing: 141 - 150 of 150 results

  1. Modular Cloning Guide

    Type
    Guide
    ...Cloning System Plant Expression Jan Lohmann 56 plasmids to create plant expression vectors containing several...Yeast Expression, Insect Expression Chen Davidovich 41 plasmids to construct multigene expression vectors...4G-Cloning Plasmid Kit Bacterial Expression, Insect Expression, Mammalian Expression Stephan Gruber Platform for...assembly of expression vectors for multi-subunit protein complexes in various hosts. Expression cassettes...Toolkit Mammalian Expression Yizhi Cai 95 plasmids to generate mammalian expression vectors for transient ...assemble mammalian expression vectors for proximity labeling experiments. Multiplex CRISPR/Cas9 Assembly Systems...Mammalian Expression Takashi Yamamoto Plasmids for construction of all-in-one CRISPR/Cas9 vectors expressing...

  2. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...much smaller proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed...al., 2024). In MagIC-cryo-EM, target proteins (expressed as a GFP fusion in this proof-of-concept study...showed that AAV-delivered CHARM could silence the expression of prion protein in mouse brains for at least...gene product; it simply turns the target protein expression off. Plus, DNA methylation is a long-lasting ...-silencing CHARM variants that stop their own expression after silencing their target — potentially improving...by targeting marker proteins that are uniquely expressed in a given cell type. For example, glial fibrillary...GFAP) is a glia-specific intermediate filament expressed in astrocytes throughout the brain, making it ...

  3. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...We generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP...plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your ...enChIP system enables us to express the CRISPR complex much easier in various cells, including primary mouse... IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. Fujita T, Fujii...

  4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ... DNA compaction, an enhancer can increase the expression of a gene that is millions of base pairs away...Putting it all together, we get the following expression for step 1: (H3K4me1 + H3K27ac) – H3K4me3 The...enhancers with CRISPR Armed with the genome math expression for NANOG enhancers, we’re ready for step 2: ...of embryonic stem cells (ESCs) with a lentiviral vector, an attenuated retrovirus in the same family as...regions and their reverse complements were parsed for spCas9 PAM sites (NGG) and then filtered based on two ...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Viral Vectors AAV Vector Guide Adeno-Associated Viral (AAV) Vector Guide Jump To: AAV Vectors Vector Production... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements, making...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...used as viral vectors for gene therapy applications. This guide contains many viral vector-specific terms...adenovirus, read our Adenoviral Vector Guide . Recombinant AAV Vectors Wild-type AAVs have been modified...Plasmids required for rAAV vector production. Created with BioRender.com. AAV Vector Production Cloning Cloning...

  6. Adenovirus Guide

    Type
    Guide
    ...Viral Vectors Adenoviral Vector Guide Adenoviral Vector Guide Jump To: Adenoviral Vectors Vector Production...capacity of rAdV vectors (~6.5 kb for first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb)...third-generation vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette ...Read our adenoviral vector guide to learn about adenoviral vector components, production and common uses...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...adenoviral (rAdV) vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus...Generations First-generation rAdV vectors The first generation of rAdV vectors was developed by removing the...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...on genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts...given library have the same backbone, but they express or target different genes. Some libraries cover...like lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen...popular libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale,...

  8. CRISPR Plasmids and Resources

    Type
    Collection
    ...visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment based on a variety...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression. RNA Targeting...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  9. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search the table for "Bacteria"....Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation of...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...Guides Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral...Lentiviral Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...models. Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically...
Showing: 141 - 150 of 150 results