We narrowed to 210 results for: cycs

1. Hot Plasmids - August 2020

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   ...dCas9-VPR repressor in hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids...

2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   ... in the 80s and 90s, Joe was that kid who used recycled notebook paper, despite the fact that it was gray...

3. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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   ...Improved Properties of FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62...

4. March for Science

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   Blog Post

   ...scientists then revise their hypotheses and the cycle begins again. No part of this process is inherently...

5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ...constant temperature, eliminating the need for thermocyclers. This is followed by in vitro transcription ...

6. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   ...observed no negative effects on cell viability, cell cycle progression, or apoptosis with Cas9/gRNA expression...

7. Deisseroth INTRSECT Collection

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   Collection

   ...Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in the Periaqueductal...Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. ...

8. Hot Plasmids: Spring 2025

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   Blog Post

   ...identification of general heat-shock proteins using IP with CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-...

9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   ...mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between...

10. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...to subject your membrane to several freeze/thaw cycles to facilitate cell lysis: alternating between 5...

11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...promoters: the BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901...

12. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...is present, the probes will ligate. Subsequent cycling through rounds of melting and annealing allows ...

13. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    Blog Post

    ... a lambda red phage, λTetR, that carries the tetracycline resistance gene and the lambda red repressor...

14. 9 tips for a successful postdoctoral experience

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    Blog Post

    ...presentations benefit from practice. If you simply recycle slides from the last time you gave a presentation...

15. Typing CRISPR Systems

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    Blog Post

    ... depth, the Jennifer Doudna lab maintains an encyclopedia of Class 2 CRISPR systems called CasPEDIA, with...

16. Antibodies 101: Flow Cytometry

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    ...modifications, determining cell health, analyzing cell cycle stages, and detecting specific populations of cells...

17. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post

    ...many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg et...

18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...harmful mutations More, due to selection from more cycles of proliferation Less Cell types All types Less...

19. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post

    ... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...

20. Delivery Methods for Generating iPSCs

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    Blog Post

    ...replicates in the cytoplasm independent of the cell cycle. A challenge of using Sendai is that since it’s ...