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We narrowed to 628 results for: erf

Showing: 141 - 160 of 628 results

  1. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...company benefits from their policy! Perfectionism When we reach for perfection, our work stalls. We become so...seems impossible to start at all. This is why perfectionists tend to also be procrastinators.  If you fall...timeframe. Tell yourself they don’t have to be perfect or even good. The goal is simply to throw out ideas...

  2. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...you may have been a little surprised at how this powerful tool is in such a small package. But don’t let... be skipped or whether selecting colonies and performing Sanger sequencing will suffice…and the answer...mostlibrary requests specifically to allow scientists to perform functional titering and optimize transduction efficiency...finally!) ready to use your transduced cells to perform your pooled library screen…but that’s a blog post...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...Cryo-EM samples can be damaged at the air-water interface (AWI) during the freezing process, resulting in...When proteins (blue) interact with the air-water interface (AWI) before vitrification, they can be completely... Small LEA proteins as an effective air-water interface protectant for fragile samples during cryo-EM ...PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded an average integration efficiency...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...multiplexing to multiscaling NIR-shifted spectrum is perfect for use in spectral multiplexing (Shcherbakova ...the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...state-of-the-art NIR fluorescent probes and in choosing the perfect one for your experiment!   Thank you to our guest...

  5. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives." Proceedings of the...However, it can be difficult to compare their performance, as most translation initiation sequences are...translation system. The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free...size and expression level can be conveniently performed through the N-terminal eGFP tag carried on all...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...constructs), in functional analysis of proteins (to perform alanine scanning mutagenesis or targeted substitution...). Such non-complementary bases at the 3’-end interfere with plasmid reconstitution, and consequently ... of primer duplications), final validation is performed by sequencing functional regions of the plasmid... at extremely low frequency), and these could interfere with the function of the resulting construct. ...

  8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...selective transgene expression. While this is a powerful experimental strategy, one of the most common ...is to know how the AAV vector you intend to use performs in your system. This includes a thorough testing...experiments requires a full understanding of vector performance in your system. It is very rare for a methods...known and unknown factors that affect vector performance in each and every system and cell type. Therefore...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...several brain regions where other serotypes may perform better (3). Brain Region  Optimal Serotype ...Cycles Of course, one cannot truly predict the perfect titer, but one of the main recommendations when...expressed that anatomical assessments can be easily performed too. While I implied that axoplasmic transport...vectors that will have substantially improved performance profiles and should be suitable for genome editing...

  10. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scholar/student: visiting from another university to perform research   Why should I try research? Personally...research, and if that’s where you’re at too, it’s a perfectly great place to begin. Here’s my advice: Reflect...Reflect on your personal interests While it’s perfectly fine to be unsure, it helps to have a vague sense ...about yourself and the world around you, it’s perfectly normal to realize that you have new interests ...

  11. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...allow scientists to use CRISPR-Cas technology to perform genome-wide knockout screens. These vectors and...utilized to create recombination template vectors, to perform multiple site mutagenesis and create complex fusion... and Chrimson, which have enabled the group to perform independent two-color optical excitation of neurons...subdivisa (CsChR), Chloromonas oogama (CoChR), and Scherffelia dubia (SdChR). ShChR, nicknamed Chronos, is blue...display. APEX2 has been shown to exhibit superior performance over APEX for both EM imaging and proximity-dependent...

  13. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition...

  14. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...We perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We...aliquoted and stored at -80oC until we are ready to perform transformations. A typical 200mL bacterial culture... of the large number of transformations being performed at Addgene, this adds up to a significant time...

  15. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing... 500ng of 293FT genomic DNA as template after performing gDNA purification and have tried conditions with...recommends using the Epicentre QuickExtract solution to perform this step to extract genomic DNA from cells. The...

  17. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...how we use next generation sequencing results to perform quality control on deposited plasmids. We’ve also...improved feature detection with an easy to use interface. We regularly use Snapgene for our quality control...region is present in the plasmid, we might need to perform a restriction digest to confirm the size of the...

  18. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...various conditions. However, this system is far from perfect. For example, If your fusion protein is expressed...GFP aggregation, which they reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1...What can you do with SunTag? Tanenbaum et al. performed a variety of different experiments in their paper...

  19. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ... While restriction cloning is still routinely performed by many labs, a variety of other cloning techniques...transformation. After transforming cells, you should perform colony PCR with vector specific primers to confirm...Resources on the Addgene Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up on Your...

  20. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ..., changing locations, or even just as part of performing a specific function. A transmembrane protein ... validated for the type of assay that you are performing. If they’re not available, the next best thing...denatured protein samples. If, however, you are performing an immunohistochemistry assay, you may want to...
Showing: 141 - 160 of 628 results