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We narrowed to 634 results for: erf

Showing: 141 - 160 of 634 results

  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...antibody. Perform a second series of wash steps. Activate the signaling molecule and perform the assay...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody...sample with the primary (unconjugated) antibody. Perform a series of wash steps to remove any unbound or...

  2. Antibodies 101: Conjugation

    Type
    Blog Post
    ...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about...conjugated antibody will have been verified to perform the same as its unconjugated version, but if you...that you will need to confirm your antibodies’ performance after the reaction.  If you are looking to conjugate...the antimicrobial agent sodium azide, which can interfere with conjugation reactions. If needed, you can...

  3. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...of Medicine. Optogenetics and chemogenetics are powerful tools to modulate the activity of neurons and ... be the best option. Fluorescent proteins can interfere with fused protein function Under some circumstances...found that the fluorescent proteins can, indeed, interfere with the correct function of the protein of interest...transgene of interest. In our studies, mCherry interfered with the transport to the membrane of the DREADDs...

  4. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... work was performed that could influence the results? The peer-review process is not perfect, so even ..., statistics, clarity in images etc)? Did they perform all of the appropriate controls? Refer back to ...misinterpretations. After all, the science was performed, written and evaluated by humans. Keeping up with...

  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...after, we learned about organs and the jobs they perform. This became foundational later on when we studied...genetically engineered neurotropic viruses emerged as powerful tools for visualizing neural connectivity (Nassi...with restricted TVA and G protein expression is a powerful development in neural tracing. Using these technologies...outputs. In general, RABV monosynaptic tracing is a powerful tool that can be used to reveal brain circuitry...

  6. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice All of these...promoter plasmids! Comparing synthetic promoters performance in mouse, monkey, and human retinas Next, the...in monkey and human retinas. By comparing the performance of promoters across these three species, the ...vectors References Juettner, J., Szabó, A., Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., ...

  7. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...throughput cloning efficiency measurements. We performed additional efficiency measurements using similarly...of the 5-, 12- or 24-fragment test systems was performed by setting up assembly reactions in which a single...re-engineered to provide improved Golden Gate performance, a series of optimization experiments for these...these more complex assemblies were performed. It was found that high efficiencies and accurate assembly...

  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...of your prep. Many plasmid prep kits recommend performing an extra wash step to remove endonucleases (see...the binding matrix could clog the matrix when performing filter based plasmid prepping. Be sure to stay...yield as these factors are generally, but not perfectly, correlated. Typical growth times for high copy... to Do a Bacterial Transformation Learn How to Perform a Diagnostic Restriction Digest Additional Resources...

  9. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...tested in yeast5. Ecological confinement involves performing experiments in a geographic area where escaped...such as malaria and dengue are currently being performed in regions that don't have resident populations...very few gene drive experiments that can't be performed with two or more confinement methods. Since molecular...scientists could not be trusted to handle such a powerful technology responsibly. An accidental release ...

  10. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... has refined our use of the BLAST web browser interface to be as efficient as possible. If you find yourself...sequences is probably the fastest BLAST search to perform and will save you time compared to other types ...parameter, as this will affect the amount of time to perform the search as well as the overall alignment results...correct!]  Since Sanger sequencing reactions are imperfect and sequence near the beginning or end of a reaction...

  11. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...LwaCas13a, which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In ...resulted in 15% C-to-U editing activity. They then performed sixteen rounds of directed evolution across the... cytidine deaminase activity. While RESCUE can perform C-to-U edits, the authors note that it retains ...whole-transcriptome RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A...

  12. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...company benefits from their policy! Perfectionism When we reach for perfection, our work stalls. We become so...seems impossible to start at all. This is why perfectionists tend to also be procrastinators.  If you fall...timeframe. Tell yourself they don’t have to be perfect or even good. The goal is simply to throw out ideas...

  13. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...you may have been a little surprised at how this powerful tool is in such a small package. But don’t let... be skipped or whether selecting colonies and performing Sanger sequencing will suffice…and the answer...mostlibrary requests specifically to allow scientists to perform functional titering and optimize transduction efficiency...finally!) ready to use your transduced cells to perform your pooled library screen…but that’s a blog post...

  14. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...multiplexing to multiscaling NIR-shifted spectrum is perfect for use in spectral multiplexing (Shcherbakova ...the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...state-of-the-art NIR fluorescent probes and in choosing the perfect one for your experiment!   Thank you to our guest...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...Cryo-EM samples can be damaged at the air-water interface (AWI) during the freezing process, resulting in...When proteins (blue) interact with the air-water interface (AWI) before vitrification, they can be completely... Small LEA proteins as an effective air-water interface protectant for fragile samples during cryo-EM ...PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded an average integration efficiency...

  16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives." Proceedings of the...However, it can be difficult to compare their performance, as most translation initiation sequences are...translation system. The gene set was carefully chosen to perform statistically relevant benchmarking of cell-free...size and expression level can be conveniently performed through the N-terminal eGFP tag carried on all...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...constructs), in functional analysis of proteins (to perform alanine scanning mutagenesis or targeted substitution...). Such non-complementary bases at the 3’-end interfere with plasmid reconstitution, and consequently ... of primer duplications), final validation is performed by sequencing functional regions of the plasmid... at extremely low frequency), and these could interfere with the function of the resulting construct. ...

  18. Important Considerations When Using AAVs

    Type
    Blog Post
    ...several brain regions where other serotypes may perform better (3). Brain Region  Optimal Serotype ...Cycles Of course, one cannot truly predict the perfect titer, but one of the main recommendations when...expressed that anatomical assessments can be easily performed too. While I implied that axoplasmic transport...vectors that will have substantially improved performance profiles and should be suitable for genome editing...

  19. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...selective transgene expression. While this is a powerful experimental strategy, one of the most common ...is to know how the AAV vector you intend to use performs in your system. This includes a thorough testing...experiments requires a full understanding of vector performance in your system. It is very rare for a methods...known and unknown factors that affect vector performance in each and every system and cell type. Therefore...

  20. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scholar/student: visiting from another university to perform research   Why should I try research? Personally...research, and if that’s where you’re at too, it’s a perfectly great place to begin. Here’s my advice: Reflect...Reflect on your personal interests While it’s perfectly fine to be unsure, it helps to have a vague sense ...about yourself and the world around you, it’s perfectly normal to realize that you have new interests ...
Showing: 141 - 160 of 634 results