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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 266 results for: his-tag

Showing: 141 - 160 of 266 results

  1. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ... who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard and essential...generating mutated sequences for site-directed mutagenesis.  Quantitative polymerase chain reaction, qPCR...interest  Molecular Cloning Site-directed mutagenesis Molecular Diagnostics qPCR Quantify starting... PCR  Plasmids 101: Colony PCR Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase...

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...antibodies against the same target; tagged protein strategies that express a tagged version of the antigen and ...compare staining of the antibody to that of the anti-tag antibody; and immunoprecipitation-mass spectrometry...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...

  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...can be found in Botman et al., 2019 . FP Tagging Yeast tagging vectors to create fusions of proteins of...plasmids to overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins...Collection Botman-Teusink Yeast FP Collection FP Tagging FP Overexpression Resources This comprehensive ...allows for constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive...Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS One, 8 (7):e67902...). Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-...

  4. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions to, and support for, this... shipped out each year.     Figure 3: Percentage of requests per year for CRISPR plasmids categorized...

  5. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...system from the Bassik lab, uses an MS2 hairpin-tagged gRNA to recruit a cytidine deaminase and induce... Available modifications include histone acetylation by p300, histone demethylation by LSD1, cytosine ...

  6. Antibody Guide

    Type
    Collection
    ...the sdAb, or by using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have ...location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry (ICC) Cell Sorting...in these assays. This method is suitable for DNA:histone modifier interactions, which have strong binding...methods such as immunofluorescence (IF), immunohistochemistry (IHC), and immunocytochemistry (ICC) use... they only have one protein of interest. Immunohistochemistry (IHC) and immunocytochemistry (ICC) IF can... can refer to both immunohistochemistry and immunocytochemistry when fluorescent signaling molecules (...

  7. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ... mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP tag, TRX, or V5)...pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags Industry Additional Resources Read Addgene's Blog...

  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...generate one molecule of GFP-tagged LC3 for every one molecule of RFP-tagged LC3∆G. Like the unmodified ...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting...

  9. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...frequent bouts with Impostor Syndrome, Joe has found his people through meetings and social media. Joe is ...conference hashtag I had, however, started a Twitter account, and was following the meeting hashtag. I noticed...Favorable conditions - Expansion of networks The last stage is Exponential Growth, named after the growth phase..., and the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual... career scientists, students, and those from historically underrepresented groups. Through such mentoring...

  10. CRISPR Guide

    Type
    Guide
    ...DNA. The system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The...in mammalian cells include: SunTag system - co-expression of epitope-tagged dCas9 and antibody-activator...acid, turn a promoter on/off, or add small protein tags. Precise Modifications Using Homology Directed Repair...genetic modification ). Browse Plasmids: Endogenous Tagging CRISPR Base Editing To overcome low HDR efficiency...also be fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR ...dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing...sequence specified by a particular gRNA. Epitope tag(s) are fused to dCas9 or gRNA for efficient purification...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ... vivo. Some researchers have used fluorescently tagged DNA-binding proteins to label certain loci, but...Kits Find Empty Backbones with Fluorescent Protein Tags ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...this rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live...

  12. Hot Plasmids - November 2023

    Type
    Blog Post
    ...lentiviral vectors to stably or inducibly express tagged iPAK4 from different promoters, specific time points...plasmids here! Lin D., Li X., et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature...externally-timed bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record...proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching an old antibody....1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian O’Neill The Cohen...created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion protein...ticker tape images could recover transcriptional histories, accurate to within 40 minutes. Figure ...

  13. CRISPR Library Amplification

    Type
    Protocol
    ...Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 µL, 10 ...Caution Electroporation involves the use of high voltages, please use caution when activating pulse and ...down at 30 ℃ overnight. Critical Ensure at this stage that no unabsorbed media drips onto the lid. Let... Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical...

  14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative regulators..., largely depends on cell type and laser voltage. The voltage describes an electric potential that can... the cell or on its surface. Bear in mind, the voltage setting of the laser impacts the magnitude of FSC...setting as needed. To figure out the appropriate voltages to observe your cells on a flow plot, you might...respectively. H, A, and W describe the shape of a histogram, which graphically represents the time and intensity...graph of photocurrent vs. time. The resulting histograms is described through maximum current (H), the...the signal strength. So, we need to adjust the voltage in the cytometer’s software in order to generate...

  15. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...hello. Keep your cards handy and make sure your name tag is always visible. Some people learn names by seeing... them. It is OK to ask someone to see their name tag if you are one of those people. Meet people in sessions...or make one!) or find the conference's Twitter hashtag. Use social media to let others know you will attend...Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships for Success...

  16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...into a vector with a large tag at the N-terminus, but you don’t have the tag already included in the vector...you can insert both the gene of interest and the tag sequences into the vector in one step without scars...In this blog post, I will go over some advantages, disadvantages, and examples of how scientists are using... common, and for good reason. They offer many advantages over the traditional restriction enzyme cloning...thereof) nor a serious time commitment. Another advantage is that this process makes it easy to generate...will be using. Some of these products may offer advantages that Gibson assembly does not such as increased... 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules...

  18. TALEN Plasmids and Kits

    Type
    Collection
    ...of a TALE, along with strong nuclear localisation tags and repress transcription initiation when targeted... plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus, a 152-residue ...the repeat domain, and C-terminal SV40 NLS and HA tags. Plasmid 47389 also contains the VP64 domain (four... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent... was recently shown that TALE fusions with the histone demethylase LSD1 (Lysine specific demethylase) ...with the FLASH method can decrease the amount of Histone H3 lysine 4 (H3K4) di-methylation at the target...

  19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate protein localization...fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein (mito-PAGFP) ...Markus, and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding...an FP-chimeric protein may be at any one of many stages of synthesis and degradation. For this reason it...reversible photoactivation - and have enabled sophisticated imaging techniques.     Irreversible photoactivation...victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains a mixed population...PA-FPs by performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry...

  20. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs...Available modifications include: histone acetylation by p300 histone demethylation by LSD1 cytosine methylation...for targeted epigenetic modification, including histone acetylation/demethylation, and cytosine methylation...
Showing: 141 - 160 of 266 results