We narrowed to 660 results for: otos

... tries to “optimize” a particular standard lab protocol. For example, someone may attempt super quick ...quick incubation times in a Western Blot protocol and then blame the antibody when no signals are obtained...so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details about...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems...

...spatiotemporal serotonin release in vivo. bioRxiv 2023.05.27.542566. Yulong Li Serotonin Serotonin (5-HT) sensor...measuring serotonin dynamics. Nat Neurosci. 2021 Apr 5. Yulong Li , J. Julius Zhu Serotonin Serotonin sensor...00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor GRAB-HTR6 A serotonergic axon-cilium synapse...Ca 2+ indicator with enhanced two-photon absorption. Neurophotonics. 2024 Apr;11(2):024207. Robert Campbell...19(2):231-241. Tommaso Patriarchi Serotonin Red and green serotonin sensors GRAB_5-HT3.0 Dual-color GRAB...12. pii: S0092-8674(20)31612-3. Lin Tian Serotonin Serotonin (5-HT) sensor psychLight Psychedelic-inspired... jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. Nat Methods...

...FSC/SSC), the detected photons can be represented through a graph of photocurrent vs. time. The resulting...electric potential that can be applied to the photomultiplier inside the cytometer to increase the electric...-mouse CD8b-PerCP-Cy5.5), which is present on cytotoxic T killer cells but not dendritic cells. Using ...

...GFP can exist in a protonated and a deprotonated state (Tsien, 1998). The deprotonated state has an absorbance... and it emits light that peaks at 508 nm. The protonated state, however, does not absorb light at 488 ...

...intensity, caused partially by fluorophore photoinstability, as well as the tendency of fluorophores to...ideal characteristics (low aggregation, high photostability, and high stability after fixation.) From the...cells in which the construct is expressed. The photostability of Brainbow-3.2 is improved due to the addition...of neural circuits, such as connections between motor neurons and the neuromuscular junction. In zebrafish...

...studies. Endotoxin contamination is minimized by using an endotoxin-free plasmid purification protocol. To ...gram-negative bacterial endotoxin is ensured to be less than 5 endotoxin units per mL. The endotoxin levels are determined...recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector preparations can ...distributed to customers. Details about our production protocols, titering methods, and quality control are described...elements and an internal control of known titer (protocol modified from Lock et al. (2014) (Link opens in...vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC). ... determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity...

...fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme for two-photon (2P...iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of...after inserting EGFP and provide step-by-step protocols for viral generation and stable cell line creation...

...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

...significantly cheaper. When using a homemade plasmid prep protocol, it cost less than $10 to make 1000 lanes worth...ladder? You can find the Tan Lab’s DNA ladder prep protocol here and the pPSU1 and the pPSU2 plasmids are ...Use the Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests ...

... new ways to learn about science careers, lab protocols, and, of course, plasmids. Today we’re trying ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...

...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...

...these DNA samples must also be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall...for your research Brush up on molecular biology protocols using our video collection ...

...viral trasduction (see the Zhang Lab's Nature Protocols Paper for more information). V2.0 This designation...variants, as well as links to the Zhang Lab's Nature Protocols Publication describing them here. References ...engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281-2308. PubMed PMID: 24157548....

... Lab TAP Tagging Protocol 97.2 KB Yamamoto PITCh Tagging System The Takashi Yamamoto lab has deposited...provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB Mendenhall...can be found in Sakuma et al., Nature Protocols, 2016 . Yamamoto Tagging Plasmids: pX330S-2-PITCh - expresses...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...tagging techniques, as well as the plasmids and protocols needed for using them, are described below. Read...2014 The Förstemann lab has provided detailed protocols for N- or C-terminal tagging in Drosophila cells...locus in C. elegans . This system uses a 10-day protocol, generates “clean” homozygous mutants with no ...

...Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...2014) (Link opens in a new window) . protoSpaceJAM protoSpaceJAM is an all-around platform for CRISPR ...cloning; injection protocol pU6-BbsI-chiRNA ; phsp70-Cas9 PDF, 109 KB Orkin and Bauer Protocol for Genomic Deletions...Resources CRISPR Software General Resources CRISPR Protocols Addgene CRISPR Resources CRISPR Plasmids Addgene's... (Link opens in a new window) A collection of protocols, materials, and publications by members of the... library amplification CRISPR pooled libraries Protocol at Addgene Church gRNA design and cloning gRNA...pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and ssODNs...

...therapy. The laws behind phage therapy, acceptable endotoxin levels, etc. can vary depending where treatment...information on individual phages. Integrating lab protocols, clinical information, phage research job postings...resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers page ...

...lower illumination settings, lowering photobleaching and phototoxicity concerns and extending the potential...

... involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...the overlap between groups.  They found that photostimulating the Kolmer-Agduhr cells was sufficient to ...Kolmer-Agduhr cells on one side of the body required photostimulation in order to drive the full symmetrical response...

...Biology Reference Find molecular biology protocols Browse our protocol video collection ...needs 5 great apps for lab life  Sharing your lab protocols using apps to save you time and track your experiments...

...production on a protocol video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I ...stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory of our...