We narrowed to 724 results for: rel

...agarose gel to confirm product presence, size, and relative quantity. End point PCR is most commonly used ...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...

...8.2 kb, and in vivo transgene expression ceases relatively quickly due to immune response against AdV. Importantly...

...genomes Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the...females do not. Also, males have sex combs on their forelegs which are black, bristly hairs. Females do not...

...Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway Clone Collection...cases arise and millions of people die from cancer-related complications each year. Despite these grim statistics...making the results of any such study as clinically relevant as possible. Connecting the research community... initiative to curate our collection of cancer-related plasmids such that each is linked to a known major...

...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ... genetic capacity of ~4.5 kb. At 4.1 kb, SpCas9 barely fits into AAV when you add in a gRNA and regulatory...

...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...

...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...

.... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...low rate, there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy...

... other Cas effectors can’t, while maintaining relatively high efficiency. These alterations are achieved...inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding...

... all the proteins in the class that showed the relative sizes and the conserved domains. This information... maybe you’re researching a topic for which no relevant or recent review exists. Once you pick a topic...format also has the benefit of exposing you to relevant researchers in the field. Finally, you can sound...from start to finish. Find the information that’s relevant to you, note it, and move on. At the beginning...

...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...

... use next-generation sequencing to measure the relative proportion of guide RNAs (gRNAs) across the population...die or differentiate Step 3 reveals which NANOG-related genes are critical to stem cell survival. Knowing...

... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise... co-transfection of these plasmids, 293T cells released transgene-containing lentiviral particles into...

... necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid...

...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ...

...and purification An affinity tag, generally a relatively small sequence of amino acids, is basically a... Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used ...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization...western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires low pH...  0.8 Affinity and Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility...

...plasmid is packaged for viral delivery, or the relative expression levels between two or more genes is...the same mRNA. The term "self-cleaving" is not entirely accurate, as these peptides are thought to function... plasmids listed in the table above (and their related plasmids) are designed to have one or more of the...fluorescent reporter for easy detection.5  Just released: Addgene's Plasmids 101 eBook And now for some...

... A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B...TRC cloning vector has a 1.9kb stuffer that is released by digestion with AgeI and EcoRI. shRNA oligos...terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids available from ... have at least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene recommends that you select...cloning vector contains a 1.9kb stuffer that is released upon digestion with EcoRI and AgeI. The oligos...