...genomes Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the...females do not. Also, males have sex combs on their forelegs which are black, bristly hairs. Females do not...

...8.2 kb, and in vivo transgene expression ceases relatively quickly due to immune response against AdV. Importantly...

...agarose gel to confirm product presence, size, and relative quantity. End point PCR is most commonly used ...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...

...attending the session, and what is their background relative to your research? Tailor your language to your... you may receive. People often ask about the relationship of your work to recently published papers or...audience just that. Explain what you do know about a related topic, and let them know while you appreciate the...collaborate with a group who presented on a topic related to your research? Find a way to get in contact ...

...Nicotiana benthamiana. N. benthamiana is a tobacco relative and a good model for plant disease because it ...tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses and wheat, but we do work... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

...practically this isolation is only feasible for relatively short DNA molecules. In order to isolate a particular...most plasmids are replicated in E. coli and are relatively small (∼3000 - 6000 basepairs) to enable easy...Because bacteria are easy to grow in a lab, divide relatively quickly, and exhibit exponential growth rates..., and thus the plasmid exists in a symbiotic relationship with the host cell. Like the bacterial chromosomal...recommend checking out these two Addgene blog posts relating to common E. coli lab strains and E. coli strains...specialized for protein expression for additional strain-related information and a more extensive strain list. Strain...endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega e14-(McrA-) recA1 endA1...

...easiest switch from fluorescent proteins given the relative simplicity of the system and the availability ...

... use next-generation sequencing to measure the relative proportion of guide RNAs (gRNAs) across the population...die or differentiate Step 3 reveals which NANOG-related genes are critical to stem cell survival. Knowing...

... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise... co-transfection of these plasmids, 293T cells released transgene-containing lentiviral particles into...

... necessary control elements for each would be relatively large. Cloning the viral genes in this plasmid...

... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...low rate, there is evidence that zeocin is never entirely detoxified, even when Sh ble is expressed (Trastoy...

... other Cas effectors can’t, while maintaining relatively high efficiency. These alterations are achieved...inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding...

.... Time Viral production in mammalian cells is relatively quick. After transfection, HEK-293T cells start... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

... all the proteins in the class that showed the relative sizes and the conserved domains. This information... maybe you’re researching a topic for which no relevant or recent review exists. Once you pick a topic...format also has the benefit of exposing you to relevant researchers in the field. Finally, you can sound...from start to finish. Find the information that’s relevant to you, note it, and move on. At the beginning...

...future edits. In contrast, since Cpf1 cleaves relatively far away from the PAM, NHEJ might retain the ...

...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...

...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...

...and purification An affinity tag, generally a relatively small sequence of amino acids, is basically a... Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used ...crystallizing it or testing its function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization...western blots, IP, co-IP, IF, flow -cytometry, but rarely used for purification as elution requires low pH...  0.8 Affinity and Purification Very small size, rarely affects function S-tag KETAAAKFERQHMDS  1.8 Solubility...

...creating and editing Wikipedia pages, OWW will be relatively easy for you to begin using. For the novice, ...and bacterial transformation. And more plasmid-related educational resources can be found in our Plasmids...the repository. These protocols show up on the related plasmid pages and can help scientists use these...

...plasmid is packaged for viral delivery, or the relative expression levels between two or more genes is...the same mRNA. The term "self-cleaving" is not entirely accurate, as these peptides are thought to function... plasmids listed in the table above (and their related plasmids) are designed to have one or more of the...fluorescent reporter for easy detection.5  Just released: Addgene's Plasmids 101 eBook And now for some...