We narrowed to 303 results for: rigi

1. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

   Type

   Blog Post

   ...genome of a group of Archaea. Consistent with this origin, the authors suggest that Cas14 could be a more...

2. Designer PUF Proteins for Any RNA Target

   Type

   Blog Post

   ...of the high fidelity version (despite what the original paper says). In their hands, BsaI-HF has produced...

3. Top 10 Open Science Developments of 2013

   Type

   Blog Post

   ...topic, and the corresponding author's country of origin, were taken into account. 7)     Science’s John...

4. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

   Type

   Blog Post

   ... details and final experimental outcome in the original publication. As you develop and confirm new gRNAs...

5. Easing the Protein Purification Process with pCri

   Type

   Blog Post

   ... thorough introduction to pCri, please see the original publication cited below. pCri plasmids are available...

6. Science as Art

   Type

   Blog Post

   ...plants and other organisms without destroying the original, which could provide a new tool to study old and...

7. Addgene is Expanding Our Viral Vector Service!

   Type

   Blog Post

   ...or in vivo testing. We recommend you check the original paper of your AAV plasmid of choice for more information...

8. What's New in CRISPR - March 2020

   Type

   Blog Post

   ...editors with minimized off-target DNA editing The original cytosine base editor, BE3, induces a low frequency...

9. An Addgene Summer Internship

   Type

   Blog Post

   ...results every week always came with surprises. Originally we thought during the first three weeks that ...

10. Build Your CRISPR Vocabulary

    Type

    Blog Post

    ... with alternating spacers and repeats, was the original impetus for studying CRISPR. Spacers: Copies of...

11. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type

    Blog Post

    ...significant improvement and simplification of the original technique, specifically in terms of initial steps...

12. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type

    Blog Post

    ... annotate promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are...

13. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type

    Blog Post

    ...usually something toxic. This gene is found on the original plasmid and either the insertion of a DNA fragment...

14. Tips for Improving Your Next Manuscript

    Type

    Blog Post

    ...guide to becoming a successful writer. When I originally talked to my PI about becoming a better writer...

15. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    ... are many such sites in the human genome). The original plasmid is empty following the removal of the ...

16. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    ...kill bacteria, or inhibit their growth. Although originally sourced from natural products, many common antibiotics...

17. History of CRISPR Cas - A tale of survival and evolution

    Type

    Blog Post

    ... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...

18. Antibodies 101: Epitope Tags

    Type

    Blog Post

    ...protein that can also be used as a tag! GFP was originally derived from jellyfish (Aequorea victoria) and...

19. Meet the New Addgene Board Members!

    Type

    Blog Post

    ...Ultragenyx Pharmaceutical in 2017. As one of the original employees at Dimension Therapeutics, she helped...

20. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    ...and colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and...