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Showing: 141 - 160 of 726 results

  1. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...out their own novel experiments in semester two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates...capabilities and laboratory and critical thinking skills (Knutson et al., 2010a,b). Classroom-based research projects...before a CURE trainee goes on to win a Nobel!   Jason Pellettieri is Professor and Chair of the Biology...Einstein Discovers America. New York./  Callahan, K., Peterson, C., et al. 2022. External collaboration results...planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J., Wallert, M., Provost, J. 2010a....and Molecular Biology Education. 38(5):317-323. Knutson, K., Smith, J., Nichols, P., Wallert, M., Provost...Education. 38(5):324-329. Kowalski, J., Hoops, G., Johnson, J. 2016. Implementation of a collaborative series...

  2. DNA Purification Without a Kit

    Type
    Blog Post
    ... is adapted from the 1979 Patterson Protocol. When published, the Patterson Protocol was shown to purify...DNA gel extraction method described in Kim and Morrison is nearly identical to that of commercially available...Purify DNA using glass filters. Image: Kim and Morrison, 2009. This method provides DNA yield and quality... kits for different purposes, with the Kim and Morrison method, you can just adjust the number of glass... protocol video library References Kim, Y., & Morrison, S.L. (2009). A Rapid and Economic In-House DNA...://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason, M.G., Wang, Y., Wee, E.J., Turni, C., Blackall,...

  3. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...troubleshooting as advised by the technical support person. This delayed the progress of the project. After...familiarize yourself with technical support. Even having personal experience with technical support through the ...general positive attitude, and an open-minded personality. These traits will help you deal with any incoming...and there are also differences in customers’ personalities and their expectations. If you are willing to...is essential for success. For possibly this same reason, it can be one of the more constant and safe corners...simple way even if you don’t exactly know why. Personal and professional development in technical support...support gives you the opportunity to develop interpersonal and communication skills. Problem solving and...

  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...therapeutic applications. piggyBac (PB) transposon: The PB transposon is a mobile genetic element that transposes...heterogeneous iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing of transgenes...heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene expression... into cells as a donor plasmid containing the transposon, along with a helper plasmid that expresses the... is that genomic alterations can occur at the transposon insertion site, so sequence-verification is required... pluripotent mouse stem cells by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363-69. PubMed...Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta, Mei Huang, Zongjin ...

  5. Deep Dive: qPCR

    Type
    Blog Post
    ...multiplex (Thellwell, 2000). See Table 1 for a comparison of features of several commonly used chemistries...sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that your reference...genes - and it is well worth your time to do one (Biassoni, 2014). At the bench As a highly sensitive technique... See Table 2, (adapted from Wong, 2018) for a comparison of different analysis methods.   Method ...defined log-linear phase   Table 2: A comparison of different qPCR analysis methods. Table adapted... master. References and Resources  References Biassoni R; Raso A. (eds.), Quantitative Real-Time PCR:...doi.org/10.1016/j.bdq.2017.11.001.Bustin SA; Benes V; Garson JA; Hellemans J; Huggett J; Kubista M; Mueller ...

  6. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ... of a two-part interview with Vini Mani and Amy Gilson from Science in the News (SITN) at Harvard University...half of our conversation with Vini Mani and Amy Gilson from SITN, we discuss some of the many things you.... Many thanks to our guests, Vini Mani and Amy Gilson. Vini Mani is a graduate student at Harvard University...You can follow Vini on twitter @vinidhra.     Amy Gilson recently received her PhD from Harvard University...SITN podcast team. You can follow Amy on twitter @aigilson.   Special thanks to our sound engineer/producer...Science Careers 10 Steps to a Perfect Science Talk 5 Reasons to Use Reddit for Science Communication ...

  7. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...arise as a result of existing professional (and personal) relationships. However, most job seekers will...pile, it may never even be reviewed regardless of personal connections to the hiring group.  Read here for... Concern” as you will not likely know the exact person monitoring submissions (usually a Human Resources...mutual connection. If the organization is small, any person at the company might be able to help you. If the...LinkedIn is one great way to find an appropriate liaison, but you can use alumni databases, friends, and...make a difference. Having your resume forwarded personally gives you a much better chance at an interview...

  8. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...competence procedures Threats to personal standing – including undermining personal integrity, destructive innuendo... analytic scientists seem to toss data-driven reasoning out the window when making decisions. We think...think scientists make choices based on logic and reason, but often our decisions are based on emotions and... these labs in the first place. This is another reason to do your research and choose wisely. From the...members fight it out PI intervenes if there are interpersonal conflicts PI has no idea who is dating whom ...choose a grad school or postdoc lab. Having a comparison can be very helpful in uncovering strengths and...

  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...University of Alberta studying with the Szymanski lab. Reasons to study how a phage recognizes its host Bacteriophages...molecules in molecular biology labs (for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages...following is a description of a technique published by Simpson, Sacher, and Szymanski in January 2016 in Viruses... for physical shearing exist as well, such as sonication and needle shearing. Essentially, any method ...Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...27536293. PubMed Central PMCID: PMC4971087. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David J., Jessica C. Sacher, and Christine M. ...

  10. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ... James M. Wilson New serotype pENN.AAV.hSyn.Cre.WPRE.hGH AAV2 Recombinase James M. Wilson New serotype...James M. Wilson New serotype pENN.AAV.CamKII 0.4.Cre.SV40 AAVrg Recombinase James M. Wilson New serotype...FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep pAAV-CaMKIIa-hM3D(Gq)-mCherry...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

  11. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...PMC4246457. 4. Davidson CJ., et al. "Improving the limit of detection for Sanger sequencing: a comparison of methodologies...although this has been improved in some studies) (Davidson et al. 2012, Tsiatis et al. 2010). As the field... approaches can provide the researcher with a reasonable level of certainly that the effects they see ... PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute at the University of Cambridge...Vouillot, Léna, Aurore Thélie, and Nicolas Pollet. "Comparison of T7E1 and surveyor mismatch cleavage assays...PubMed Central. 5. Tsiatis, Athanasios C., et al. "Comparison of Sanger sequencing, pyrosequencing, and melting...

  12. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...such a resource when updates depend on a single person. With thousands of scientists routinely using and...Protein Property Visualization allows for visual comparison of FPs and how they relate to one another. .  ...you or your lab use frequently) for the sake of comparison or ease of retrieval.  These collection pages...of your choosing!), and they can be downloaded in JSON or CSV format to quickly retrieve all of the information...store and represent within-study photobleaching comparisons.  We very much appreciate feedback, so try out...plasmids Find plasmids for FRET Check out the Michael Davidson Fluorescent Protein Collection ...

  13. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...be a graduate student, postdoc, professor, etc. Reasons to pursue a visiting role The main motivation to...can’t obtain at your current institute. A common reason is hands-on training in a technique that only a...hard to find, or inaccessible to you for another reason. Visiting would allow you to train and acquire ...data you need on the new instrumentation. Bonus reasons Visiting gives you access to possible new collaborations... willing to host you! If there is someone you personally know or your lab collaborates with that can fulfill...work together to ensure your expectations are reasonable, your reagents are onsite before you need them...

  14. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Non-science favorites  The Mistborn series by Brandon Sanderson "The Mistborn series is the first 3 books of a...funny, bittersweet, and shocking at times."     - Jason Snair, Product Designer Sarum: The Novel of England...skeptical reader of historical fiction (I can personally attest to this!). Following history from the ...participated in a book club before, but I had a few reasons for launching this one. On a purely selfish level...contentious, discussions. I presented the first book, Nicholson Baker’s The Anthologist. The book ended up literally...Prejudice, and some more obscure treasures, like Tove Jansson’s The True Deceiver. There have been books that ...

  15. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...If cloning methods had personalities, SLIC (sequence- and ligation-independent cloning) would be a true...also be beneficial. SLIC is most often compared to Gibson assembly, a cloning method also based on homologous... recombination. The higher temperature at which Gibson assembly takes place may limit formation of secondary... of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase is much less...less expensive than the enzymes required for Gibson assembly. SLIC is a standardized method for multi-fragment... Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning...

  16. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... Connelly JP, Pruett-Miller SM, Blanc V, Davidson NO, Madison BB (2017) A stable but reversible integrated...assaying CRISPR/Cas-stimulated HDR activity) The Madison lab created SRIRACCHA in 2017 to enrich and identify...Wen et al., 2018). SRIRACCHA uses the Piggybac transposon to stably integrate a reporter gene that contains...your cells.  A plasmid that contains Cas9. The Madison lab also created an inducible SRIRACCHA system ... experiment that targeted the gene NFE2L2, the Madison lab found that H2B-GFP expressing cells exhibited...

  17. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ... think more favorably of scientists they know personally Scientists in your field will know about grants...Job hunting outside of academia is all driven by personal connections What can you do to get the most from...whose company they enjoy on a professional and personal level.  Here are some tips to get out of the rut...free places to order cards. There are a few good reasons to have a card but two of the best are: if you ...with a more general intro until you know if the person you are talking to is really in your direct area...attend. Meet some people online to follow up with in person. Email lab members that have moved on to see if...

  18. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ... https://doi.org/10.1021/acssynbio.6b00031. Iverson, Sonya V., et al. “CIDAR MoClo: Improved MoClo Assembly...proteins, then the CIDAR Kit is an excellent option (Iverson et al., 2016). CIDAR offers a one-pot reaction ...developers noticed that studies frequently made comparisons between CRISPR components expressed from different...nature of the Fragmid system allows for direct comparisons of CRISPR parts. The Fragmid system was used ...fungi, bacteria, protists, plants, and animals (Chiasson et al., 2019). Many plasmid elements such as promoters...Crossref, https://doi.org/10.1016/j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden...

  19. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that happens...Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn is a place to... to build relationships. If you don’t add a personal note, you waste an opportunity to start things out...Connect.” If you click this box, it will send an unpersonalized, generic invitation. Don’t try to connect from...have had success with this and as long as you personalize the invitation with your intention for connecting...

  20. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...CRISPR-Cas systems.   Four years later, the Sontheimer and Davidson labs discovered three anti-CRISPRs for ...Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, Sontheimer EJ, Davidson AR, Maxwell KL, Doudna JA (2017) A Broad-Spectrum...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR (2016) Naturally Occurring Off-Switches...
Showing: 141 - 160 of 726 results