We narrowed to 911 results for: TRO

...Protocol Recombinant Antibody Purification Protocol Introduction Immunocytochemistry is a technique that uses...Day 3-4: Fix and label cells Equipment Pipette controller Pipette tips and pipettes Rocking platform Tweezers... appropriate fluorescent filters. Tips and Troubleshooting The optimal fixation method will vary depending...functioning as expected and specific, include a positive control sample that you know expresses the protein, such...express the protein of interest, and a negative control sample such as cells that do not express the protein...

...Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol can be used to titer lentivirus...titer of your lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...All of the cells in the untransduced (negative) control well should be killed and no colonies should be...appropriate antibiotic. When the untransduced (negative control) cells have died and colonies are visible in the...

...Gel Purification Molecular Biology Reference Introduction This protocol describes how to extract RNA from...unlike using a p1000 tip it will give you more control of where you’re aiming your tip in the tube. Precipitate...the quality of your RNA sample(s) using a spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer...

...gel electrophoresis, which separates DNA by their length in base pairs. Following electrophoresis, you...Restriction Digest of Plasmid DNA Agarose Gel Electrophoresis DNA Ligation Background Information Gel purification...Protocol: Gel Purification Follow the Agarose Gel Electrophoresis Protocol with the following amendments: Notes...

... Technology Protocol Agarose Gel Electrophoresis Gel electrophoresis is the standard laboratory procedure...visualization and purification. Agarose Gel Electrophoresis Protocol Purifying DNA from an Agarose Gel ...DNA fragments based on size. Following gel electrophoresis, you can cut DNA bands out of the agarose gel...

...Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol can be used to titer lentiviral... titer of your lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Inverted fluorescence...dilution with one well remaining as the untransduced control). Count the cells in the remaining well, a cell...

...Purifying DNA from an Agarose Gel DNA Ligation Introduction Restriction enzyme digestion takes advantage...your restriction digest results. Equipment Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot...manufacturer's instructions) Gel loading dye Electrophoresis buffer Pipet tips Procedure Select restriction...visualize the results of your digest, conduct gel electrophoresis . Tips and FAQ Restriction enzymes MUST be ...

...Using a Light Microscope Using a Light Microscope Intro to the Lab Bench Check out more protocols and videos...videos to help you get started in the lab! Introduction Microscopes are emblematic of biological research... cheap and accessible) to massive transmission electron microscopes that allow us to see cellular components...resolution when your image is in focus, which you can control using the focus knobs on the side of the microscope...

...Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol can be used to generate stable...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...plate will hold 5 dilutions plus one 'no virus' control well). Perform a "reverse transduction" by seeding...selection should last at least as long as it takes the control (untransduced) cells to completely die. After that...

...express Cas9 and are included as a control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was a gift...Virus Blog Posts Addgene Protocols Viral Service Introduction This protocol describes how to generate a monoclonal...µL single channel pipette CO 2 incubator Pipet controller Hazardous waste container Microscope Hemocytometer...