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We narrowed to 469 results for: Lif

Showing: 161 - 180 of 469 results

  1. Hot Plasmids - November 2023

    Type
    Blog Post
    ...multiplex immunolabeling of brain. eLife, 8, e43322. doi: 10.7554/eLife.43322. PMID: 30667360....also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

  4. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ... A degree in the life sciences prepares one for numerous non-academic careers. Still, many start their...are many roles out there that require exactly what life science PhDs can offer: vast practical lab expertise...support make excellent use of the skills developed by life science PhDs. In my days as junior postdoc I was...

  5. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...including the development of crops with longer shelf life and the development of disease-resistant crops to...expression include the ubiquitously expressing cauliflower mosaic virus 35S promoter (CaMV 35S) or ubiquitin...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...

  6. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...though they are aware that only 14.3% of PhDs in the life sciences end up in tenure track position 5 years...academic researchers themselves(44% of postdocs in the life sciences name faculty-research as their preferred... and enjoyable path for people from all walks of life will continue having conversations like those at...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough...whichever targets you like. Plus, indirect IF amplifies the signal since multiple secondary antibodies...

  8. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...make a huge difference for your future career and life. You might see a lab head as choosing you, but in...I take to get to work?”; we often make important life decisions without much logic - even choosing a training...whom Expanding your skillset Scientists are lifelong learners. I believe we won’t be happy if we aren...

  9. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...register enables the design of primer pairs that PCR amplify in some states but not others. Because samples ...not be very informative – each primer pair will amplify even if its target states are only represented ... determine the fraction of registers that are amplified by a particular primer pair. Then, by leveraging...

  10. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...expression profile at multiple steps from plasmid amplification in bacteria to transgene expression in vivo....are typically employed during viral plasmid amplification (Fischer, KB. 2019). This means that all DIO...of spontaneous reversion during AAV plasmid amplification. On the left homologous pairs of scrambled cre-recombinase...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...is an assistant professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies...choosing. Tools such as SapTrap Builder greatly simplifies design of these constructs, allowing novice researchers...is an assistant professor at the University of California, Santa Cruz where his group focuses on gene regulation...

  12. Finding Your Science Policy Path

    Type
    Blog Post
    .... Though I had spent the first few decades of my life following a very straightforward conveyor belt from...all the career spaces I explored for my post-PhD life, the science policy community is the one that was...these activities leading to a rich day-to-day work life.  Action Items:  Join the National Science Policy...

  13. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    .../bd-spectrum-viewer.   But what is the real-life advantage of a brighter fluorophore? Strong brightness...en-us/resources/bd-spectrum-viewer.   To make life easier, always try to use fluorophores with distant...immunity. When he's not in the lab, Paul enjoys weightlifting, sailing, and reading.   More resources on ...

  14. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...Stephanie Hays, a researcher at the University of California Berkeley. It’s been half a year since the march...rift where our integrity is questioned and cuts to life-saving research are being proposed.  It's our responsibility...studying an evolutionary arms race at University of California, Berkeley. She is a scientist with a passion ...

  15. Antibody Guide

    Type
    Guide
    ...Affinity Reagents Handling Visualization Signal Amplification Application Overview Quantification Methods ...present on the Fc region. This allows for the amplification of signals by having multiple secondary antibodies...on the antibody and conjugate chosen. Signal Amplification The amount of signal needed from a specific ...region of primary antibodies allows for signal amplification through the indirect detection method. In the...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...

  17. Cloning

    Type
    Guide
    ...discovery. With molecular cloning scientists can amplify and manipulate genes of interest and then insert...restriction sites to any piece of DNA during PCR amplification, allowing for it to then be digested and ligated...a single recombination reaction, drastically simplifying the process and reducing the amount of time required...). To do this your DNA fragment must first be amplified with specific Gateway attB1 and attB2 sites attached...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... by cells and to the uncoating step in the viral life cycle. Using AAV for neuronal tracing AAV vectors...plasmids at Addgene). The Gradinaru Lab at the California Institute of Technology generated novel AAVs ...Together with scientists at the University of California, Berkeley, the Janelia Research Campus also developed...

  19. Editor's Choice, October 2016

    Type
    Blog Post
    ...to our latest podcast all about graduate student life and how establishing a thriving graduate student...Healthcare Consulting: A Door to the Business of Life Sciences 6 Tips for Grant Writing 5 Reasons to Use...

  20. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...and talk to us and wish them the best of luck in life beyond graduate school. Ben J. Vincent is a Ph.D...fellow in clinical genetics at the University of California, Los Angeles. She enjoys chromosome biology and...
Showing: 161 - 180 of 469 results