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We narrowed to 545 results for: SEC

Showing: 161 - 180 of 545 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    ...neurons. Your choice of AAV will depend upon which section you’re looking at (striatum, hippocampus and cortex...single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression begins....PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting step for efficient...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...knowledge could be very useful for drug discovery, to dissect a complex signaling pathway, and to identify what... accelerate progression into clinical trials. Secondary screens  If you have generated a list of candidate...candidate genes from a full genome screen, a smaller, secondary, CRISPR screen could be used to validate the list...

  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...switch or photoconversion usually takes less than a second. The first successful irreversible PA-FP reported...activate the first PA-FP does not photobleach the second, and vice versa. Finally, as every microscope will...favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different varieties...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from...Toronto KnockOut (TKO) CRISPR Library. This complex second-generation CRISPR lentiviral library targets nearly...target ~15,000 genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing...

  5. CRISPR Guide Update

    Type
    Blog Post
    ... disposal. With this update, we have added new sections to share and describe some incredible new CRISPR...Highlights include a much-anticipated prime editing section and additions describing CRISPR’s ability to make...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    ... primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature, which...providing flexibility in assay design. Conjugated secondary antibodies are relatively cheap and easy to source...: Selecting the Right AntibodyAntibodies 101: Secondary Antibodies Resources on Addgene.org Addgene's ...

  7. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ... any further ado, we present to you: our first, second, and third place winners!  1st Place: Krampus Saves...creativity, also receives an honorable mention for their second submission.    Our Cultured Warrior (made by Nate...

  8. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...about Addgene. The page is divided into three main sections: Protocols To complement our written protocol... see here? Give us suggestions in the comments section below! Additional Resources on the Addgene Blog...

  10. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ... public and help make science more transparent. Second, SITN helps graduate students develop their communication...engage the public - let us know in the comments section! More information about SITN: DayCon event website...

  11. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...eight modular repeats, each of which binds a consecutive base in its RNA target. By changing amino acids...specificity of other RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA...

  13. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News... get involved in science communication. In this second half of our conversation with Vini Mani and Amy...

  14. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

  15. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...DNA—to cut, nick, or bind. DNA CRISPR array: The section of the bacterial chromosome that stores a genetic...trans-activating CRISPR RNA” base pairs to the repeat section of the crRNA, forming the repeat:antirepeat duplex...

  16. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...and there are unique benefits to using sdAbs as secondary antibodies. sdAbs, however, really shine in vivo...the Addgene blog Introduction to Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic...

  17. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ... beauty of serendipity often emerges at the intersections of disciplines. Cast your net wider, explore...Maintaining openness and looseness of spirit The second crucial element in harnessing the power of serendipity...

  18. Hot Plasmids - October 2022

    Type
    Blog Post
    ...missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here. Here's...expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc [9E10] page for more details....
Showing: 161 - 180 of 545 results