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  4. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...4D nucleome, the organization of the nucleus in space and time, and to understanding how nuclear organization...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  6. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...the correct solution is fundamental, and it will impact the time you will be able to store your sample .... The two solutions may differ though in their capacity to maintain DNA stability during storage over ...

  7. Fostering communication in remote collaborations

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    Blog Post
    ...feel included in the project and accelerates the pace at which problems can be addressed and new ideas...that work best for them. This also accelerates the pace of research by circumventing the slower, sequential...

  8. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...repository! This includes the first Addgene prep packaged in the AAV9-X1.1 serotype.  In rodents, AAV9-...You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP....

  9. An Addgene Summer Internship

    Type
    Blog Post
    ...first week at Addgene, I had to get used to the workspaces, as well as complete the lab safety and cybersecurity...safety, general work skills — such as using Google workspace — and got the chance to work on my very own research...

  10. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...experiment and the organism you are working in. The spacing of genes and whether or not it’s ok to disrupt ...the known and unknown microbial coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554...

  11. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ..., and disease. In order to expand their model’s impact, organoid users have recently begun to develop ...these novel biosensors could have a major economic impact.   Design and mechanism of action of the...

  13. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...aliquots in a kit with a huge amount of wasteful packaging.  Turns out, one could make a lifetime supply ...indefinitely at room temperature (thus also saving freezer space).  The procedure becomes less attractive when noting...

  14. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...blockbuster known as the CRISPR (clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes...genetic material next to the species-specific protospacer adjacent motif (PAM). Once the CRISPR/Cas complex...

  16. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...time. “At an early age, I was fascinated by the impact that medicines can have on human health. As a teenager...organizations are less common, they can be very impactful. At Novartis, Stegmeier deposited the ClonTracer...

  18. The Strength of Story Telling

    Type
    Blog Post
    ...engage their readers or listeners thus expanding the impact and appreciation for their research. Long story...judgement. Eventually, we published the paper in a high-impact journal and it worked out for all of us.” This ...

  19. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...ColE1 A Relaxed pR6K ~15-20 R6K* C Stringent pACYC ~10 p15A B Relaxed pSC101 ~5 pSC101 C Stringent...phage-derived ori that allows for the replication and packaging of ssDNA into phage particles. Plasmids with phage-derived...

  20. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...Developmental biology caught me early on: a simple egg is packed with information. I am fascinated with how developmental... from. Put your skills and interests into your backpack, and step out there into the galaxy. TL;DR: Many...

  23. Don’t Wait To Launch Your Health Science Startup

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    Blog Post
    ...tech companies using only laptops and some server space. But what if you want to be an entrepreneur in ...you start at a disadvantage in the health science space if you don’t have an advanced degree behind your...

  24. Another Pathway into Cells: iTOP

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    Blog Post
    ...to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...directly with proteins removes the limitations of packaging size associated with viral vectors. For these ...

  27. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...menstrual pads while we talked about the environmental impact of disposable products. A great deal of scientific...Braman, D., & Mandel, G. (2012). The polarizing impact of science literacy and numeracy on perceived climate...

  29. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and ...plasmid DNA that you can use along with lentiviral packaging plasmids to create your own lentivirus stocks....

  30. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...promoters, has limited the number of gRNAs that can be packaged into viral vectors for potential in vivo applications...therapeutic potential of the DAP array, the Gao Lab packaged MBE and MPE elements for deliveries via adeno-...
Showing: 241 - 270 of 556 results