Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 395 results for: POR C

Showing: 241 - 270 of 395 results

  1. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the plasmid map...research opportunities to their students and other members of the MCC community. They support educators...local hub of educators teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional...

  2. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    .... DOI: https://doi.org/10.1080/rsob.180241 Vanni, C., et al., Unifying the known and unknown microbial...fundamentally the reporter construct monitors the expression of a nearby gene. The reporter gene serves as... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...stimulate the expression of the reporter. The system is made up of a reporter gene flanked by a minimal promoter...promoter – all rely on the random integration of a reporter construct in close proximity to an endogenous ...example, if green fluorescent protein (GFP) was the reporter gene and green cells were observed in the kidneys... for with an uncharacterized gene. The actual reporter construct can be something phenotypic (such as...

  3. Antibody Guide

    Type
    Collection
    ...stored at 4 °C for short-term storage. For long-term storage they should be stored at -20 °C in aliquots...conjugated to fluorophores should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate... after thawing and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies used...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating...

  4. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...of our most popular mammalian expression vectors, c-Flag pcDNA3, comes from the pcDNA series. It has the...consider as you make your choice. The first and most important thing you need to know is your expression system...have to make sure that your plasmid has been incorporated into the host organism, usually achieved with...basal expression level, induce expression at an opportune time or go all out with constitutive high level...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

  5. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... Deverman BE, Matho KS, Cetin A, Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J...’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such...the Cre recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...sites lets researchers control the spatial and temporal activation of a transgene. Figure 2: FLEX...structure when transcribed which aids in nuclear export of mRNA and helps increase the expression of your...polyadenylation or polyA signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript...

  6. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht...and have always been, a community resource that supports both toolmakers and tool users. We encourage you...Educational Resources As part of our commitment to supporting your research, Addgene is creating an array of...Through our incredible community, including your support, we have enabled scientists to easily share plasmids...Studies Hybridoma Bank, a nonprofit that has been supporting hybridoma sharing within the community for decades...Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative grant (U24NS119916 )...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...∆G, however, can not be conjugated to PE due to a C-terminal glycine deletion, so it remains in the cytosol... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux...makes Keima useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

  8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... antibodies aren’t free to bind endogenous IgGs. (C) A third solution is to use Fab fragments against ...primary antibody that is directly conjugated to your reporter circumvents the issue. However, directly conjugated...which uses just a primary antibody that has your reporter (in this case a fluorophore) directly attached... as effective for other techniques, but it is important to be aware that the issue is not unique to IHC...

  9. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...released from nuclei by a short incubation at 37 °C. These short DNA fragments can then be purified for...MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity for mouse... rather than to rely on nuclei extraction. They report that this change offers a significant improvement...

  10. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...) antibody-dependent cellular cytotoxicity (ADCC) c) antibody-dependent cellular phagocytosis (ADCP). ...starting a cascade of events that results in the pores being opened in the pathogen’s cell membrane (Dunkelberger... when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody dependent...

  11. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...under the United States Internal Revenue Code 501(c)(3). That means we were formed to benefit the public...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

  12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...receptors. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-78827-9 Breen, C. J., Raverdeau, M...Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C., Patel, R., Lu, R., Macklin, J. J., Keller, P. J....protein function and cellular components: It's important to assess how dye-conjugation affects protein ... interpretation of experimental results. It's important to consider how dyes can influence receptor conformation...efficacy of these ligands can be affected by the incorporation of fluorophores, as seen in studies involving...wavelengths for green, red, and far-red dyes is important for intracellular fluorescence live imaging ​​(...from cells under certain conditions, which is an important consideration for assay design (Breen et al., ...

  13. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...T-regs) into their microenvironment by producing C-C motif chemokine 22 (CCL22) and TGFβ. T-regs are suppressive...still many important questions in this exciting field of research. One of the most important: How will ...commute to and invade multiple organs, and most importantly escape the immune mechanisms of the host. The...against cancer. Sipuleucel-T by the Dendreon Corporation is currently the only FDA-approved DC therapy...therapies themselves can be made more effective. Some important factors researchers are beginning to focus on ... of cell types. This cell heterogeneity is an important element of successful tumor progression. A tumor...), and ability to metastasize. There have been reports of clinical trials going amiss and patient deaths...

  15. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...allow the digestion to take place for an hour at 37°C. This should be enough time to completely digest the...overhangs, insert and vector purification, and, most importantly, excessive time requirements. FastCloning is ...

  17. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... new scientific observations. References 1. Rago C, Vogelstein B, Bunz F. Genetic knockouts and knockins...Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat ...of the gene with multiple gRNAs, but the most important is that you could avoid potential off target artifacts...you could run another gel that highlights the important results (for example, if you needed to make a ... Despite the shifting landscape of research opportunities, it’s an exciting time to be in the lab. Based...

  18. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources ...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49411 pCFD4-U6:1_U6:3tandemgRNAs dU6:1 and dU6:3...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin...transcription Virmilion none, need Cas9 plasmid Bullock and Port 49409 pCFD2-dU6:2gRNA dU6:2 BbsI Injection or in...transcription Virmilion none, need Cas9 plasmid Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, ...

  19. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein of interest with a fluorescent reporter on the N- or C-terminus of the protein. One downside of...doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim C, Boulanger CM (2012) Flow cytometry: retrospective...a pressurized buffer called sheath fluid and transported through tubes or capillaries to a laser. As the...

  20. An Addgene Summer Internship

    Type
    Blog Post
    ...biochemistry. There, I had exchanged contact with Amy C., Addgene’s intern coordinator shortly after hearing...capstone project report and presentation, during the final week at Addgene. This report shared my work ...Coming from an environment with limited opportunities and support, puts into perspective just how much one...hearing her talk about Addgene’s internship opportunity taking place next summer.  I applied to Addgene ...to differentiate which clone had successfully incorporated Syntaxin3 into its backbone. Once we had gotten...

  21. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...light. Previous iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs...back in with the Addgene blog for future award opportunities. Xin Zhou: Fluorescent Light-inducible Proteins...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...consisting of sfGFP fused to a His-tagged ABC transporter known to bind to the HMO of interest. Fatima ...

  22. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...depositing can be grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks...drop-down menu to indicate whether the tag is on the N or C terminus and whether it was present in the backbone...be entirely verified by sequencing. Upload any support files that will make it easier for scientists to...

  23. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...Antonini, M.-J., Rosenfeld, D., Park, J., Garwood, I. C., Yan, W., Niroui, F., Fink, Y., Chandrakasan, A.,...included in them for downstream control – this is important later! Next, additional components are added -...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

  24. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ... I as this enzyme recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests double stranded DNA at this ...salt causes arcing during electroporation which would cause the electroporation to fail). When incubating...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...

  25. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...Gelsinger, D. R., Vo, P. L. H., Klompe, S. E., Ronda, C., Wang, H. H., & Sternberg, S. H. (2024). Bacterial...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....streamlined, one-size-fits-most approach. evoCAST is an important step toward that kind of gene treatment. The researchers...

  26. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic...encodes it. That DNA sequence then needs to be incorporated into a host cell (through a process called transformation...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and...

  28. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... system is capable of robust expression of high G+C content genes, as validated through monitoring the...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...

  30. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...arrays (e.g. for spacers A, B, and C, you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab...expressed, the gRNAs cannot be released, adding temporal and/or spatial control to the system. pSQT1313...plasmids, which contain various promoters and reporters, and subsequently inserted into a Cas9-containing...-in-one CRISPR/Cas9 vector system.” Scientific Reports 4 (2014): 5400. PubMed PMID: 24954249. PubMed Central... Find plasmids from this paper at Addgene. 10. Port, Fillip, et al. “Optimized CRISPR/Cas tools for ...
Showing: 241 - 270 of 395 results