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We narrowed to 642 results for: ato

Showing: 241 - 270 of 642 results

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...plasmid backbones into which you can clone your regulatory element or gene of interest to create a luciferase...transformation plasmids with G418 selection Find nematode transgenesis plasmids with Neomycin selection ...

  3. The Pleiades Promoter Project

    Type
    Collection
    ...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective expression... RA, Goldowitz D, Wasserman WW, Simpson EM. A regulatory toolbox of MiniPromoters to drive selective expression...

  4. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive cell ...Ting JT, Zeng H, Tasic B. Enhancer viruses for combinatorial cell-subclass-specific labeling. Neuron. 2021...

  5. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...Addgene contracted with a professional scientific translator, L. Denise Roche, to edit the translation. We...  Many thanks to:  Daniel Mejías Sánchez, PhD, translator  Denise Roche, PhD, MD, editor, ldeniseroche@...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    .... While certain cell types, such as principal excitatory cells and glia, have been successfully targeted...’s lab has developed a new AAV approach using regulatory elements that restrict expression to this cell...wild type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor) fusion protein...

  8. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    ...structure is strikingly similar to the design model at atomic resolution” (Kuhlman and Dantas et al., 2003). ...). Design of a Novel Globular Protein Fold with Atomic-Level Accuracy. Science, 302(5649), 1364–1368. ...

  9. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ... retrograde serotype, and a dTomato under the control of the E6 regulatory element, which targets it to...

  10. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise and endurance...altogether. Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors...

  13. Let There Be LITE Plasmids

    Type
    Blog Post
    ...customizable DNA-binding domains based on transcription-activator-like effectors (TALEs) as anchors for genome engineering...and modular,” Konermann said. “Transcriptional activators are easy to expand or combine with different ...

  14. Editor's Choice, August 2016

    Type
    Blog Post
    ... bit of a Twitter storm surrounding NgAgo, its creator, Han Chunyu of Hebei University of Science and ...Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect Science...

  15. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

  16. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...hour, we feasted on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...

  18. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...for students, postdocs, and young Principal Investigators (PIs). We've interviewed Addgene depositors ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...

  19. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  20. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...applications. Also, purifying GST by affinity chromatography requires proper folding of the tag, which prevents...to be purified by single step metal affinity chromatography. Purification of His fusion proteins from E...

  21. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases Rylan Larsen New serotype...multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New serotype ...

  22. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...line is known to be genetically manipulated, its anatomical origin, if cell line has ever been the target...informative. Spoiler alert: HeLa is the most common contaminator line.  For those wanting to access Cellosaurus...

  26. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...of DNA that can move around in a genome. In a laboratory setting, transposons can be used to both introduce...use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...

  28. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

  30. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that...Albert Cheng and Mark Wanner from the Jackson Laboratory for Genomic Medicine described Casilio, a system...
Showing: 241 - 270 of 642 results