We narrowed to 386 results for: nans

1. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

   Type

   Blog Post

   ...fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging...

2. Viral Vectors 101: Parts of the AAV Transfer Plasmid

   Type

   Blog Post

   .... Front Neuroanat 13: . https://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, ...

3. CRISPR Challenges: Standardization and Homology Directed Repair

   Type

   Blog Post

   ...genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi, and Eric A. Hendrickson...

4. Antibodies 101: Epitope Availability

   Type

   Blog Post

   ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

5. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   ...Hauser BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, Schmidt AG, Iafrate AJ, Naranbhai...

6. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   ...drivePut your cultures in the shakerTurn the LEDs onAnd when you wantYou can just turn them offNo need for...

7. Antibodies 101: Epitope Tags

   Type

   Blog Post

   ...genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you are fortunate...

8. Addgene's Top 10 Blog Posts of 2015

   Type

   Blog Post

   ...Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and tired of having...

9. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

   Type

   Blog Post

   ...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored...

10. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    ...University of Western Ontario studying plant-based recombinant pharmaceuticals. Follow him on Twitter @AdamChinFatt...

11. Advice for Moving Into Sales After Your Science Postdoc

    Type

    Blog Post

    ...marketing gives you a great introduction to the financial world. It teaches you about selling products which...

12. Hot Plasmids: FRET-Based Biosensors

    Type

    Blog Post

    ...contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors...

13. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    ... a means of altering cell fates and generating dominant phenotypes., Development 118, 401–15 (1993). PubMed...

14. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    ...Zolotukhin S. A simplified purification protocol for recombinant adeno-associated virus vectors. Mol Ther Methods...

15. Celebrate National Book Month with Addgene

    Type

    Blog Post

    ... PhD, majored in Biochemistry or International Finance. A book club could lead to smart, sometimes contentious...

16. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    ...reporters have been developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase...

17. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type

    Blog Post

    ... PCSK9 paper, though not featuring a clinical pregnancy, was rejected by at least one academic journal...

18. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    ...little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990). More specifically, they were engaging...

19. Cultivating Community Science at BosLab

    Type

    Blog Post

    ...experiments. For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept...

20. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    ...proper use requires careful amplification and maintenance of good representation. Addgene wishes you luck...

21. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type

    Blog Post

    ...Wuchty S, Jones BF, Uzzi B (2007) The Increasing Dominance of Teams in Production of Knowledge. Science 316...

22. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    ...of this repair bias is a fascinating (and yet unanswered) question. Our recent paper (Richardson et al...

23. Plasmids 101: Mammalian Vectors

    Type

    Blog Post

    ...is typically more (double) than that used for maintenance of a transfected cell line.  Keep these tips ...

24. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    ...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

25. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    ...Comprehensive Set of MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319...

26. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    ...Nature 560.7717 (2018): 248. PubMed PMID: 30069054. Sadanand, Saheli. "EvolvR-ing to targeted mutagenesis."...

27. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    ...JB Mol Cell. 2009 Mar 27;33(6):717-26. Reporter-nanobody fusions (RANbodies) as versatile, small, sensitive...

28. AAV Q&A with Tim Miles

    Type

    Blog Post

    ... he kindly took some time to address all your unanswered questions via text! (well, maybe not all of them...

29. Antibodies 101: ChIP

    Type

    Blog Post

    ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...

30. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    ... on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics: Antibodies...