We narrowed to 642 results for: Emb

...adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction Bunz Return to top Adenoviral...using the AdEasy™ System Content last reviewed: 12 November 2025 Do you have suggestions for other plasmids...

...proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777...

...going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ...

..., the “Reproducibility for Everyone” team was assembled when it was realized that interactive workshops...connected, this diverse and international team assembled the right people at the right time. Be inclusive...

.... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ...

...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...

...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these...

... lab members. We can set ourselves up for success by giving explicit instructions to lab members through...

...something that is actually useful to you. When embarking on a new literature search, try to find a recent... rudimentary, but it works for me. I can often remember which paper I need to open, but when I can’t it...

...they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone to begin considering...papers, published in Molecular Systems Biology in November, Voigt and Alex Nielsen describe what’s possible...

...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

...encoded by mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(...cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...

...with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to...pSNAP-CaaX Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A ...

...laboratory experience is focused on classical embryology techniques such as basic observation and dissections... that will bind to complementary mRNA within an embryo or tissue. Once the riboprobes are created, the...

...have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be used to... The researchers tested several scenarios from embryo development to myeloid proliferation in acute myeloid...

...TET, can, like the iron ball-and-chain that they resemble, actually impede your investigation into the regulation...CpG so that nothing else can interact with it. Remember, the goal of this experiment is to expose an unmethylated...

...variants:   Figure 2: Inner mitochondrial membranes imaged with structured illumination microscopy...2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https...

...sensors available that will localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an ...the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References and Resources...

...will be held on Friday 16th and Saturday 17th September respectively. Join us to discuss advocacy efforts...scientists, both young and established, as they embark on or continue their careers in science-related...

...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran off the...hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they are making fairly...

...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

...to de novo assembly of the unmapped reads, employing our bioinformatics software to assemble the reads ...

...start life as fertilized eggs that develop into embryos and then become larvae. There are three larval ...importantly, this knowledge will stop you from embarking on a fruitless journey of trying to get males ...

...this case the cell needs to be fixed and the cell membrane permeabilized in order for the antibodies to reach...Parton's Lab (University of Queensland) to observe membrane trafficking events in vivo. Locate your protein...

... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Sep;11(9):713-20. doi: https://doi.org/10.1038/nchembio.1869  Nabet B, Roberts JM, Buckley DL, Paulk J...

...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using ...

...sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning vector (deposited... allows labs to create accounts in which their members can share protcols, sign-up for shared equipment...

...else you’d like to say to our readers? Patron: Remember plants. Every time you look at your plate, think...Journal. A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner...

...101784 SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T...AICSDP-42 mTagRFPT CAAX domain of K-Ras Plasma Membrane 114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin...

...likely due to quarantining {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls=False, loop=False...

... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...