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Showing: 321 - 360 of 621 results

  2. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in...appropriately named as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating louse Cymothoa...

  5. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ... former Co-director of Science in the News, and member of the SITN podcast team. You can follow Vini on...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on...

  7. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...all of the Addgene employees, the Addgene board members, vendors, Addgene supporters, and of course, Executive...integral part in its success. The Addgene board members were also recognized for their essential advice...

  8. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...filled with family, food, and travel, and you can’t remember where you put your flow cytometry protocol. Or...Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your protocols, wouldn...

  9. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...participate in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in...the beginning, I spent a lot of time shadowing members of my team to learn what I was to do for my time...

  11. An Addgene Summer Internship

    Type
    Blog Post
    ...for the first time, being introduced to all the members around the office one by one, I knew that I belonged...right concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running...

  12. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...

  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...performed with a probe surrounded by a semipermeable membrane, which is inserted into the region of the brain...passive diffusion of DA across the semipermeable membrane from the extracellular fluid into the perfused...

  15. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...came to visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run ..., the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family, including...

  16. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...

  17. What the HEK?

    Type
    Blog Post
    ...Ever wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly...cell line model for cancer-specific research. Membrane receptors and ion channels are often exogenously...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...These convert the amino acid tyrosine to betanin, a member of a class of pigments primarily found in plants...skill that takes practice and is difficult for new members of the lab or collaborators unfamiliar with these...

  19. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies on lysosomal...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

  21. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...the stochasticity of molecular interactions and embedding individual diversity to get from gamete to a visibly...replication and gene transcription during vertebrate embryogenesis. My interests, though, have proven unruly. Instead...

  22. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

  23. Plasmid Cloning by PCR

    Type
    Blog Post
    ...overhangs to your insert of interest for Gibson assembly. The steps following primer design and the PCR...to ligate our cDNA into the recipient plasmid. Remember to insert your DNA in the correct orientation ...

  24. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...make sure to rinse off the surface of the filter membrane with the surrounding solution to ensure excess...excess viral particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating...

  25. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...a CAAX sequence to bind the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry...SunTag-based system tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs usually...

  27. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...customers, software developers, as well as team members in automation, sales, marketing, and more! This... up. Finish any communications with my UK team members before they log off for the day. 1:45 PM--Write...

  28. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ... is important and how it should change how the members of your audience think or act.   To address that...substitute for feedback from others.  Seek out members of your target audience and ask for their feedback...

  29. Antibodies 101: ChIP

    Type
    Blog Post
    ...that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies ...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References and Resources...

  30. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

  32. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...rmmAkm6PQd — Elizabeth Kenkel (@ElizabethKenkel) December 5, 2018 How social media has helped me in science... researcher’s work day. It’s also important to remember that it’s not the amount of time you spend on ...

  33. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...collection for plasmids containing that enzyme, and embedded in the References lists on every page you can ...

  37. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru...includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available from...

  38. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

  39. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...
Showing: 321 - 360 of 621 results