...entirely new affinity reagent.    Fig. 1: A comparison of an antibody (left) to several alternative ...  Rachel Leeson...

...interest and its localization in the membrane. The lesson we learned from these studies is that the tag protein...Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (1988) Purification of ...

...molecular biology and cloning, since they have reasonable introductions to lots of molecular biology,” ... Rachel Leeson...

...world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa.   Figure 2: Comparison between a full IgG antibody and common antigen...

...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ...

...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield...

...not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson...

...cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with the hope that...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're working...

...cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the...

...making them available to all researchers for a reasonable fee. We at NURSA recognize the importance of ...at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular...

...gene and cell-based therapies in the new era of personalized medicine (2017).   1st Gen AdVs are stripped...gene and cell-based therapies in the new era of personalized medicine." Genes & diseases 4.2 (2017): 43-63...

...Why study neural connectivity? One of the early lessons many of us learned in biology is that the body’... 3. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic...

...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...20463753. PubMed Central PMCID: PMC3152950. 3. Jacobson, Samuel G., et al. "Improvement and decline in...

...of data together indicate that 35-pieces is a reasonable upper limit for Golden Gate Assembly size. Ultimately...  Rachel Leeson...

... Maya Peters Kostman in May 2020 and updated by Jason Nasse in July 2023.  References and Resources References...Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson S (2019) Ultrapotent chemogenetics for research...

...antibodies recognize some rat primary antibodies (Erickson 1993). It is always better to choose primary antibodies...Publications. https://doi.org/10.1177/002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). ...

...consists of highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged...degradation.      New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...and Casɸ-3. This system is notable for several reasons. Casɸ is only ~70kDa, with a molecular weight roughly...the library here New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...and inhibitory synaptic proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal...library from Addgene New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...! it, or Bing it, but, let’s face it, there’s a reason nearly 70% of all internet searches are done via...potentially faulty product. Please remember that the person at the other end of that email you’re writing is...

...and so should be handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element...

...panelist or audience member from taking over. My own personal rule is that I do not let the panelists introduce...stop talking and redirect the conversation. The person you cut off might be miffed but the other 200 audience...

...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...are interested in experiencing Cas9 up close and personal, visit the 3D Molecular Designs web site. Or, ...

...consider performing PEG precipitation. Instead of sonicating the cell pellet, I lyse the cells with this AAV... Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production...

...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...identified.     New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer EJ, Weng Z, Gao G, Xue W (2015) Adenovirus-Mediated...

...Why do these organisms have chromoproteins? One reason may be that chromoproteins protect corals from ...fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure...

...resources References Pyhtila, B., Kasowitz, S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination...  Rachel Leeson...

...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

...between two molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities...Gene Tagging in Human Cells Check Out the Michael Davidson Fluorescent Protein Collection Get Tips for Using...

...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources on Addgene.org CRISPR ...

... A., Sousa, A. A., Tsai, S. Q., Bengtsson, N. E., Lööv, C., Ingelsson, M., Chamberlain, J. S., Corey, ...

...to capitalize on your experience. No matter the reason for attending, your preparation and day-of game...hear or understand what we are being asked. I am personally guilty of this, and have many times answered ...

...science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter...  Rachel Leeson...

... figure by figure organization with a different person responsible for each figure. Others still are more... avenues including casual request via email, in person, or instant messaging but we as scientists often...

...why should cell biologists talk to chemists? One reason is something called “the photon budget”: each sample...Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits ...

...opposed to four separate plasmids), there are a few reasons arguing against this approach. First, this would...PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription and replication strategies...

...philosophy of  “work hard, play hard.” Part of the reason we sincerely like spending time together is that...work” all the time. She was a wise lady for many reasons, but this saying is especially true. If your event...

... and for a very brief period of time, the only, person to know a brand new fact is often enough to keep...learning to “speak science,” and virtually every person you talked to during the day was also a scientist...

...Pk1] in a western blot assay. Image reused from Anderson et al., 2024 under CC BY license.    Find ...Find Anti-V5 [IPI-SV5-Pk1] Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R...

...proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify ...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...

... Quick tips for writing a good cover letter Personalize your cover letter to the job and job requirements...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024.     Additional Resources on the Addgene...

...Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant...  Rachel Leeson...

...becoming an expert in your field and are the perfect person to be writing the review! Doing so is a great way...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...

...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

...References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...

...of false interpretation in cell culture for this reason. Unreproducible results are rife. Misidentified...PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins. "Henrietta Lacks,...

...consider using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are...11736655. Pubmed Central PMCID: PMC1222268. 7. Aronson, Deborah E., Lindsey M. Costantini, and Erik L....

...summarized in a short blog post, but some examples I’m personally interested in include: intracranial self-stimulation... to proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different...

...act on DNA, or are enzymatically active. For this reason, recombinant proteins are typically expressed in...culture from reaching the desired density within a reasonable time-frame. For these cases, some strains carry...