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We narrowed to 494 results for: Ank

Showing: 161 - 180 of 494 results
  1. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...deliver light pulses to the target brain region. Thankfully, due to their prominence, the preparation of ...performing optogenetics behavioral experiments. Thank you to our guest blogger Derek Simon! Derek P....
  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...alterations are achieved through DNA degradation, thanks to the unique capabilities of Cas3 and its partner...CRISPR type I-E. Created with BioRender.com. Thanks to this complex, developing Cas3 tools has been...
  3. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...transmitting and sensing electrical potential. Thankfully, there is a tool to measure these electrical ...specific types of cells, such as neuron subtypes, thanks to viral serotype specificity during delivery (...
  4. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor bacteria...in the recipient cell, the CRISPRi DNA (purple) flanked by left and right Tn7 end sequences (green) integrates...
  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... spent organizing, an hour is earned (Benjamin Franklin)! It’s good practice to label your plates and/...autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana Khan Khilji! Sana Khan...
  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...cells. These plasmids use fluorescent proteins flanked by long regions of homology to the gene of interest...and protein-protein interactions. Additionally, thanks to the modular arrangement of vectors included ...
  7. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post
    ...because of our CRISPR depositors and requestors. Thank you!  Want to dive deeper into Addgene’s CRISPR ... The CRISPR Journal (Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions...
  8. PhD Applications After COVID

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    Blog Post
    ...provide them with a CV or transcripts if requested Thank them, and eagerly await a reply. It’s a good idea...interest. Benefits of Online and Hybrid Options Thanks to the much more developed virtual environment ...
  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...depositors to provide plasmid maps whenever possible. GenBank files can also be uploaded onto our plasmid pages... resources provided by the depositor including genbank files and protocols here. Articles Citing this ...
  10. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (... system under normal and diseased conditions.  Thank you to our guest bloggers, Jae Lee and Pantelis ...
  11. Antibody Guide

    Type
    Guide
    ...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...which controls for your antibody’s specificity. A blank (contains water or buffer instead of a biological...Visualization methods should employ positive, negative, and blank controls. Antibody Applications - Cell Sorting Methods...with only one antibody, for each antibody used. Blank controls in the form of sample buffer must also ...
  12. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential viral genes. How it...blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...
  13. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...know how it goes! Acknowledgements: I’d like to thank the PTA of Butler Elementary for trusting me with...with Math & Science Night, and a special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of...
  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats (LTRs) that facilitate...Bernaudin F, Girot R, Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel...
  15. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...These primers are designed to anneal to sites that flank the insert site. A positive clone will produce a...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...
Showing: 161 - 180 of 494 results