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Showing: 161 - 180 of 497 results

  1. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...authors are asked to add an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software...publishing your manuscript, or add your own tips below. Thank You to Our Guest Blogger! Maaike Pols is a Developmental...

  2. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ... provide detailed evidence of knowledge new to mankind. And you probably understand how much faster it...communicate. It’s okay (and encouraged) to leave blank spaces or to gloss over some aspects when needed...

  3. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... lost in a sea of data, my mind went completely blank. After several seconds of truly panicked silence... so they could understand it. When my mind went blank, my options were to stop talking until I could present...

  4. To Each HIS Own

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    Blog Post
    ...and can elute proteins with up to 95% purity (Janknecht 1991, Hochuli 1988). In this purification approach...Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting single...use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting single stranded...

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...library was developed when Carrie was a post-doc in Frank Stegmeier’s lab in Novartis Oncology.  Bhang did...ways you put the ClonTracer library to use! Many thanks to Carrie and Novartis for taking the time to talk...

  8. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...whole segment—a GFP tag and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into... use genomic tagging in your experiments. Many thanks to our guest bloggers from the Allen Institute ...

  10. CRISPR in the Clinic

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    Blog Post
    ...isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate functional...Cas9." Cell Stem Cell. 13, 659-662 (2013). Gerald Schwank et al. "Functional Repair of CFTR by CRISPR/Cas9...

  11. Design Tips for Prime Editing

    Type
    Blog Post
    .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson, J. W., Newby...j.cell.2014.02.001 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson...

  12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...site within the genome using homology arms that flank the gene of interest. In either case, a positive... sites are inserted directly into the genome to flank the gene that will be deleted following Cre-mediated...

  13. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...deliver light pulses to the target brain region. Thankfully, due to their prominence, the preparation of ...performing optogenetics behavioral experiments. Thank you to our guest blogger Derek Simon! Derek P....

  15. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor bacteria...in the recipient cell, the CRISPRi DNA (purple) flanked by left and right Tn7 end sequences (green) integrates...

  17. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...cells. These plasmids use fluorescent proteins flanked by long regions of homology to the gene of interest...and protein-protein interactions. Additionally, thanks to the modular arrangement of vectors included ...

  18. PhD Applications After COVID

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    Blog Post
    ...provide them with a CV or transcripts if requested Thank them, and eagerly await a reply. It’s a good idea...interest. Benefits of Online and Hybrid Options Thanks to the much more developed virtual environment ...

  19. Degrading DNA with Cascade-Cas3

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    Blog Post
    ...alterations are achieved through DNA degradation, thanks to the unique capabilities of Cas3 and its partner...CRISPR type I-E. Created with BioRender.com. Thanks to this complex, developing Cas3 tools has been...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...because of our CRISPR depositors and requestors. Thank you!  Want to dive deeper into Addgene’s CRISPR ... The CRISPR Journal (Pyhtila et al., 2023). Our thanks to Rodolphe Barrangou for his authorial contributions...
Showing: 161 - 180 of 497 results