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Showing: 161 - 180 of 401 results

  1. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ... Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines...to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...

  6. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...the viral titer (the number of viral particles capable of infecting a host cell) can be tuned so that ...Viral vector platforms within the gene therapy landscape. Sig Transduct Target Ther 6, 53 (2021). DOI: ...

  7. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency Transient...Mouse Cells and Hematopoietic Cells Low Packaging Capacity (~4.5kb From basic science to clinical applications...

  8. Clear and Accessible Writing

    Type
    Blog Post
    ...densities are captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40%...

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...fluorescent in situ hybridization (FISH) and chromosome capture suffer from low resolution and can’t be used in...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...

  12. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made...Cas7 and Cas11 domains. Cas7-11 cuts ssRNA and is capable of pre-crRNA self-processing. Functionally, it ...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...). Bacteria need special fertility genes to be capable of conjugation, and these are encoded by certain...conserve energy, these specialized degradative capabilities are often tightly regulated to be active only...

  15. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...you’re simply attending, but uncertain how to capitalize on your experience. No matter the reason for ...bullet points and elaborate if your audience seems captivated. Expertly navigate questions Answering questions...

  16. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...thin sheet to illuminate a fluorescent sample and captured the reflected light using a different objective...Ultramicroscope was developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...would permit point mutations in RNA which could recapitulate or rescue known pathogenic alleles, or introduce...REPAIRv1 to merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    ...hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA, and filters can be stacked...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters. Image...

  20. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...likely do! Give our summary table a look for a quick recap of the pros and cons of each antibiotic and happy...novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins ...
Showing: 161 - 180 of 401 results