Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 706 results for: ESC;

Showing: 161 - 180 of 706 results

  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has two...bind the SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule...contains an HA-binding scFv that’s fused to a fluorescent protein such as GFP. The HA frankenbody works...anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of ...A, Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody ...frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies  Read about...SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein. Resources on...

  2. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ... determine if CRISPR/Cas9 escape occurs in an animal model. If these escape mutations are frequent, the...HIV-1 infected T-cells to see if they could be rescued by CRISPR. CD4+ T-cells were isolated from healthy...their results, Wang et al. showed that HIV-1 could escape from CRISPR/Cas9-induced modification targeting...either the LTRs or essential genes. Many of these escape mutations were located near the Cas9 cut site, ...1 infection, which would make it easier for an “escape” virus to propagate and infect adjacent cells. ... Inhibit HIV-1 Replication and Accelerate Viral Escape. Cell Rep. 2016 Apr 5. PubMed PMID: 27068471. Ramanan...

  3. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ... friends on the Cambridge 2010 iGEM team that describes the basics of Gibson assembly as a parody of “...assembly The Gibson assembly technique was first described by Dr. Daniel Gibson and colleagues at the J. ...combined with the popular CRISPR technology and is described in a recent publication by the Lockey lab (Wang...followed by the standard Gibson assembly techniques described above, this resulted in the direct and seamless...using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015), in which very large...challenging. Other homology based technologies We've described Sequence and Ligation Independent Cloning (SLIC...homology based assembly methods that have been described--CPEC (circular polymerase extension cloning) ...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...were named after Dr. Theodor Escherich, the scientist who first described them in 1885. E. coli are mainly...right tags to your gene, and may have even put a fluorescent protein downstream, separated by an IRES element...process of replicating your new plasmid: the Escherichia coli bacteria. It’s hard to count the number ...commercial lab strains of E. coli used today are descended from two individual isolates, the K-12 strain ...mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine methylase...Expression Systems. Check out our companion post describing protein expression strains, where we go over ...

  7. What's New in CRISPR - March 2020

    Type
    Blog Post
    ... in yeast Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent stem cells... base editor plasmids CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in...adapted from Guzzo et al., 2020. Caulobacter crescentus is a popular model organism for studying the ...stem cells Lindy Barrett’s lab developed new fluorescently labeled piggyBac vectors to deliver uniform ... lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and ...that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. Read the paper in Scientific...

  8. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ... and allow for noninvasive live imaging with fluorescent tags and activation of light sensing proteins...distinct behaviors such as spontaneous swimming and escape reflex. These traits, coupled with short generation...zebrafish at 5 days post fertilization, including escape behavior and spontaneous swimming. When startled...zebrafish react by performing a distinct C-bend to escape possible danger (see figure). Larvae also perform...in response to any specific stimulus. While the escape response neural pathway is well characterized, ... kind of swimming behavior, spontaneous swim or escape, was elicited when these different neuron groups...

  9. RUBY-Red Siliques

    Type
    Blog Post
    ...categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually...would only then kill the desired plants! PCR or fluorescent markers are currently used in these situations...from heterozygous individuals. Only 1/4 of the descendants of the heterozygous plants, therefore, are useful...   I can do this by expressing a gene that rescues the mutations, using RUBY as a selection marker...mutation but lacking both RUBY and the construct that rescues the mutation. I will be able to easily choose the...could be achieved much easier than by PCR or a fluorescent microscope. First, red CRISPR-positive transformants...

  10. Pouring LB Agar Plates

    Type
    Protocol
    ...that way for ~10 min. This will allow any steam to escape from the autoclave and will cool your gel-mix slightly...

  13. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...and instead are conjugated to reporters (i.e. fluorescent dyes, HRP, etc. ) after purification. Antibodies...signal amplification occurs when HRP converts a fluorescently labeled tyramide molecule into a highly reactive...linked to residues within close proximity thus fluorescently labeling them. HRP RANbody staining results ... ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to lower background...and tissue sections Amplifying weak signals of fluorescent protein (i.e. GFP, RFP) Detecting GFP reconstitution... from Penn State Need help picking the right fluorescence microscopy technique to use? Check out this ...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items from the...32675376/ mGold, the most photostable yellow fluorescent protein to date By Justin Ng High-throughput ...High-throughput screening methods, such as fluorescence-activated cell sorting (FACS) helps researchers sift through...million cell types expressing variants of Yellow Fluorescent Protein (YFP). The team identified a new YFP ...Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand break repair assay...the introduction & resolution of two DSBs in a fluorescent reporter. Find the plasmids here Yongsub Kim...

  15. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...study a wide variety of in vivo conditional gene rescue experiments, as well as for improvement of optogenetic...additional uses for FLEx switches: Conditional rescue of a gene knockout: To test if the phenotype you...by coding sequence of the gene you would like to rescue with in the antisense orientation (let’s call the... to test whether or not expressing GeneR alone rescues the phenotype being studied. Introduction of ...functional mutation in vivo: Similar to a conditional rescue experiment with a wildtype copy of a gene, you ...presence of Cre, GFP expression is lost, allowing for rescued expression of GeneR.   Sometimes also referred...

  16. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...in this post: Bright and photostable yellow fluorescent protein mGold2 Viral delivery of TnpB for plant...connections with ATLAS Bright and photostable yellow fluorescent protein mGold2 By Mike Lacy Get your sunglasses...these proteins are bright! Most current yellow fluorescent proteins tend to photobleach rapidly, which hinders...St-Pierre recently developed a pair of new yellow fluorescent proteins: mGold2s and mGold2t (Lee & Lai, et ...using mVenus or mCitrine, or if existing yellow fluorescent proteins just haven’t met your needs, consider...et al. (2025). Bright and photostable yellow fluorescent proteins for extended imaging. Nature Communications...

  17. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...plasmid, how will it be sent to me? Description: This describes how the plasmid or other product (kit...useful to me? Plasmid purpose: This is a brief description of what the plasmid was designed and used for...vector’s plasmid number here. Gene/insert: This describes what has been inserted into the backbone, and ... sequencing. Growth in Bacteria: This section describes how to grow the bacterial strain in which the ...associated, such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can ...

  18. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically... distance between the target antigen and the fluorescent label, which can reduce the potential image resolution... resolution of super-resolution fluorescence microscopy. Their size also limits their ability to penetrate...penetration and decreases the distance between a fluorescent tag and the target antigen to about 2 nm, which...review. If you just want to use the nanobodies described in Pleiner et al, the key steps for generating...: Purified nanobodies are then tagged with a fluorescent dye or HRP via maleimide-labeling. Maleimide-...

  19. Plasmids 101: Protein tags

    Type
    Blog Post
    ...Which Fluorescent Protein Should I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent Protein...Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used as tags for...LVPRGS Cleaves between Arg and Gly residues PreScission LEVLFQGP Cleaves between the Gln and Gly residues...examples. The tables list more common tags than are described in the post, but have been categorized to help...

  20. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...experience and skills would help you do the job as described. Let’s imagine you’re applying to a job that ... your cover letter, you can expand on that by describing how you developed and used a ddPCR assay to answer...start by looking at the requirements in the job description. For each requirement, ask yourself if you have...exactly what you would want to talk about! You could describe your organizational system, your rationale for...company/organization]. I am a [adjective that describes you] scientist with over [number] years of experience...the university]” Include keywords from the job description to help the hiring manager quickly make the connection...
Showing: 161 - 180 of 706 results