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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 469 results for: LARGE

Showing: 161 - 180 of 469 results

  1. Protocol - Bacterial Transformation

    Type
    Protocol
    ...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...

  3. Antibodies 101: Validation

    Type
    Blog Post
    ...1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets already...CRISPR/Cas9. You check the mutation and it causes a large deletion that removes the entire exon that codes...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...migration resistance and improve separation. For large proteins, reduce the percentage of acrylamide. To... and typically takes less than an hour. However large proteins typically do not transfer as well using...

  5. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...mutations behind. Furthermore, piggyBac™ offers a large cargo-carrying capacity (over 200 kb has been demonstrated...recombinase. However, the use of negative selection largely overcomes this obstacle. Furthermore, we have found...a cell line with the edit of interest. With the larger piggyBac™ donor vector, it is possible to make ...

  6. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... no good treatment. The dystrophin gene is very large (79 exons), but much of the sequence appears to ...lifespan. This work should be done in mouse and large animal models with mutations in human-relevant exons...

  10. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting that scientists...the more friends they have in common. Only the largest connected part of the follower network is shown...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold fully occupied with GFP has...

  14. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less...

  16. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... offer the most flexibility for expression in a large number of cell types and are useful if you're going...several different systems or want to transduce a large number of different cells. Promoters such as chicken-beta-actin...

  18. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...discovered. These proteins are characterized by large DNA recognition sites, which makes them highly specific...agriculture, its portrayal in popular fiction has been largely negative. But these negative depictions serve as...

  19. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...interest.   Advantages Disadvantages Can carry large transgenes (>8 kb) Potential for generation of replication-competent...have been developed, but these vectors are quite large and you might get lower viral titer when using them...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...techniques like CLIP require a specific antibody and a large number of cells to analyze. A new technique from...trying to activate target genes, the relatively large size of dCas9-VPR limits one’s ability to package...their ability to generate both small (< 100 bp) and large (up to 120 kb) deletions in N. benthamiana and Arabidopsis...higher frequency and could be detected by PCR while large deletions were relatively rare. The vectors are ...human kinase domain constructs developed through a large-scale expression screen that can be used to generate...anchored to amylose resin for solid-phase synthesis of larger fusions through SpyTag/SpyCatcher and SnoopTag/...of target cells. The piggybac system has a much larger cargo capacity than lentivirus and is capable of...
Showing: 161 - 180 of 469 results