Rabies and Neuronal Tracing
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Blog Post
...with a deletion-mutant rabies virus. Retrograde neuronal tracing with a deletion-mutant rabies virus. Pubmed...
We narrowed to 402 results for: Mut
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Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...proteins have acquired divergent functions due to mutations accumulated over time? Undergraduates can help...because the S. cerevisiae deletion strains are ∆ura3 mutants and cannot grow without uracil supplementation ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...indicated in the “Species Other” column. Relevant Mutations Indicate the amino acid change as well as any...functional consequences of that change. For example, “Mutated aspartate 123 to Lysine (D123K), lowers biosensor... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...orientation limiting FRET efficiency, circular permutation (Baird et al., 1999) of the fluorescent protein...References 1. Baird, Geoffrey S., et al. "Circular permutation and receptor insertion within green fluorescent... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...additional SapTrap plasmids used for introducing point mutations that was published in a microPublication (Schwartz... E. M. "SapTrap vectors for introducing point mutations with unc- 119 + selection." MicroPublication 1... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post... if you want to knock out a gene, a frameshift mutation introduced anywhere within the early coding region... hand, if you are trying to knock-in a patient mutation or need to make a precise amino acid change, there... -
CRISPR in the Clinic
TypeBlog Post...co-injection of Cas9 and a single-guide RNA targeting the mutant allele. When all was said and done, the researchers...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...genes with many modifications. We look for point mutations, truncations, and insertions that could compromise...compromise function. When we do find mutations, we check to see if they affect the translated amino acid. ... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...your supervisor’s viewpoint and brainstorm about mutually beneficial solutions Your supervisor might not... his or her viewpoint, and discuss options for mutually beneficial solutions. If you package your challenges... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...promoter. The resolution step leads to the desired mutation and excision of the vector backbone. To do this...Characterization of a Blue Chromoprotein and Its Yellow Mutant from the Sea AnemoneCnidopus Japonicus. Journal... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post...due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability and yield. If a plasmid...using older plates could increase plasmid loss or mutation. Let it incubate Increasing the incubation time... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog Post... E. The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations. Science... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...BLAST (blastx) search to check for expected ORFs, mutations or truncations. A primary advantage of blastx ...database. Another benefit is that a frameshift mutation present in the ORF is readily apparent when viewing... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...outlined below (or emission wavelenght range). By mutating GFP, the variants blue FP (BFP), cyan FP (CFP)...breakdown of GFP, it's variants, and their relevant mutations, check out Marcy's post from last week on GFP.... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...which contains multiple point mutations in the nuclease domain. These mutations result in an inactive RNA-... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors two surface exposed...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...success of BiFC experiments. The ideal control is a mutated variant of one or both of your PIPs in which the...requires that you empirically test a number of mutants to identify those that inhibit BiFC in your cellular... -
Plasmids 101: Colony PCR
TypeBlog Post... but won’t tell you if your products have any mutations. There are lots of different cloning strategies...expected sized PCR product doesn’t mean there aren’t mutations in your insert. Make sure to submit multiple positive... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...the deletion reduces the risk of insertional mutagenesis by limiting the interaction between viral LTR...potential for oncogenesis through insertional mutagenesis. These risks are largely based upon the vector... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog Post... the sequence, normally through the synonymous mutation of a codon. You can domesticate the sites yourself...colony PCR electrophoresis), is free of undesired mutations, and that you have the correct four-base overhangs...