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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 567 results for: PAC;

Showing: 161 - 180 of 567 results

  1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our SpCas9 containing...Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific modifications... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...

  2. Bioinformatics at Addgene

    Type
    Blog Post
    ...complete package, all of the pieces of software that depend on each other for the entire package to run....in-house software has been crucial for us to keep pace with our current need to analyze nearly 300 sequences...sequencing data collection and analysis eventually outpaced our software, even with continuous updates. The...

  3. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...differing customs processes, and the distances the packages needed to travel meant the accessibility varied...such as using couriers that refill dry ice if the package stays in transit for a certain number of days and...distributor who, among other things, could ensure our packages navigated customs and arrived at their destination...for tools such as empty backbones, lentiviral packaging systems, and luciferase expression. On individual...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...take up essentially all of an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods...the chosen acceptor vector. The vectors are then packaged into AAV virions via transient transfection of...the viral vector. This delivery method frees up space in the AAV allowing a researcher to fill it with...

  5. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...many of the same restrictions, like limited cargo space (~4.7 kb) for your genetic payload — there are a... cell types not of interest. Systemic capsids packaging Cre-dependent transgenes can also be paired with...enhancers, and switches all take up precious cargo space in AAV vectors. Dual expression systems, which use...to be administered concurrently to minimize the impact of neutralizing antibodies developed in response...promoter/enhancer screening. However, AAV-PHP.AX packages viral genomes at a lower efficiency than AAV9 ...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,...

  6. Retrograde AAV viral preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV...Berkeley). Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  7. Finding Your Science Policy Path

    Type
    Blog Post
    ...rich and diverse workforce. Out of all the career spaces I explored for my post-PhD life, the science policy...webinar via the NC Science Policy student group SciPolPack for a more detailed discussion on how to to define...to chat with someone working in a science policy space you’re interested in. Many fellowship programs can...her work focuses on incentivizing the real-world impacts of health services research. She is particularly...

  8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...recombination between homologous pairs before viral packaging even begins. This spontaneous recombination persists...2020). In general, we observe that 0.1 - 0.8% of packaged viral genomes contain a recombined/functional ...which can lead to off-target expression of the packaged transgene in neurons and glia.   Check the recombination...v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination...

  9. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...color":"#0052a9","css":"#0052a9","hex":"#0052a9","opacity":100,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82..."css":"rgba(0, 82, 169, 75%)","hex":"#0052a9","opacity":75,"rgb":"rgb(0, 82, 169)","rgba":"rgba(0, 82,...color":"#ffffff","css":"#ffffff","hex":"#ffffff","opacity":100,"rgb":"rgb(255, 255, 255)","rgba":"rgba(255...–296. https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S., & Liu, D. R. (2015). Methods for the directed...

  10. Biosensor AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV ... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  11. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Fisher 26402 pF141 pAcGFP1 SOD1WT SOD1 GFP CMV ALS Elizabeth Fisher 26403 pF142 pAcGFP1 SOD1 A4V SOD1 GFP...Fisher 26404 pF143 pAcGFP1 SOD1G37R SOD1 GFP CMV ALS Elizabeth Fisher 26405 pF144 pAcGFP1 SOD1G85R SOD1 GFP... GFP CMV ALS Elizabeth Fisher 26406 pF145 pAcGFP1 SOD1G93A SOD1 GFP CMV ALS Elizabeth Fisher 26407 pF146...Prnp_SAM2 PRNP U6 Dementia Walker Jackson 79259 pAc5.1B-lambdaN-HA-DmeIF4G-V5His6 EIF4G1 His, HA, V5 Ac5...IDR-mCherry TBP Parkinson's Denes Hnisz 146392 pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G EIF4G His, HA...HA, V5 Ac5 Parkinson's Elisa Izaurralde 146493 pAc5.1B-lambdaN-HA-DmeIF4Ga-Nterm_H EIF4G His, HA, V5 Ac5... Ac5 Parkinson's Elisa Izaurralde 146577 pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I EIF4G His, HA, V5 Ac5 ...

  12. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...environmental impact on a scale of 1-10: the lower the score, the lower the environmental impact in terms ... product? How can we consider the environmental impact of those processes? Who even regulates them? You...

  13. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...lentiviral capsid-based bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression...aptamer which binds an aptamer binding protein for packaging into a lentivirus-like particle. Read the paper...paper in Nucleic Acids Research Find the gRNA and packaging plasmids   Cytosine deaminase for RNA editing...

  14. Adenovirus Plasmids

    Type
    Collection
    ...adenoviruses in research include their large packaging capacity (>8 kb), high titers, and high levels of ...molecule can then be transfected into mammalian packaging cell lines to generate adenovirus particles. Read...

  15. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...this time more visible and thereby have a positive impact on science. ReFigure saves time, knowledge and ...definitely give you credit for it and increase your impact! Download ReFigure extension and visit ReFigure.org... with Joe Akin. She envisions a world where the impact of teaching and doing science is rapid, concrete...

  16. Advice for Choosing a Research Project

    Type
    Blog Post
    ...the focus shifts to choosing an unexplored and impactful research topic that aligns with your interests...when selecting a research project. What will the impact be in a best case scenario? If you get your dream...what will your paper look like? What will be the impact on your field? You want to choose a research question...

  17. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ... 101131 pACP-GPI Control Plasmid ACP/MCP-tag Control for cell surface localization 101128 pACP-ADRβ2 Control...backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for...

  18. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...around the world were closed and not receiving packages. So the entire team mobilized to figure out how...subsequent fluctuations in distribution. I don’t have space in this blog to call out every Addgene Team because...workforce for when we need to be working at full capacity to serve the scientific community. First, we applied...

  19. What the HEK?

    Type
    Blog Post
    ... here.   Virus production HEKs are the go-to ‘packaging cells’ for viral production because they express...need only transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing...the cargo of interest, and HEKs will produce and package viral particles. The viral particles are then filtered...

  20. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Biology and, on occasion, would meet in the same space where BosLab exists today. Back then, it consisted...Kendall square, but we saw the potential in the space and decided to reactivate it. We started out by ...projects at BosLab. Some are entrepreneurs using our space to perform early-stage experiments. For example,...
Showing: 161 - 180 of 567 results