We narrowed to 559 results for: SEC

...neurons. Your choice of AAV will depend upon which section you’re looking at (striatum, hippocampus and cortex...single stranded DNA viruses, AAVs normally require second strand synthesis before gene expression begins....PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is a rate-limiting step for efficient...

...switch or photoconversion usually takes less than a second. The first successful irreversible PA-FP reported...activate the first PA-FP does not photobleach the second, and vice versa. Finally, as every microscope will...favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different varieties...

...knowledge could be very useful for drug discovery, to dissect a complex signaling pathway, and to identify what... accelerate progression into clinical trials. Secondary screens  If you have generated a list of candidate...candidate genes from a full genome screen, a smaller, secondary, CRISPR screen could be used to validate the list...

...was cloned into the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from...Toronto KnockOut (TKO) CRISPR Library. This complex second-generation CRISPR lentiviral library targets nearly...target ~15,000 genes with 6 gRNAs per gene. The second is a "supplementary" library that contains an additional...tet-inducible OsTIR1 plasmid is also available). Second, they co-transfected short-arm donor vectors containing...

... disposal. With this update, we have added new sections to share and describe some incredible new CRISPR...Highlights include a much-anticipated prime editing section and additions describing CRISPR’s ability to make...

... primary antibody overnight at 4 °C and their secondary antibody for 1–2 hrs at room temperature, which...providing flexibility in assay design. Conjugated secondary antibodies are relatively cheap and easy to source...: Selecting the Right AntibodyAntibodies 101: Secondary Antibodies Resources on Addgene.org Addgene's ...

... any further ado, we present to you: our first, second, and third place winners!  1st Place: Krampus Saves...creativity, also receives an honorable mention for their second submission.    Our Cultured Warrior (made by Nate...

...about Addgene. The page is divided into three main sections: Protocols To complement our written protocol... see here? Give us suggestions in the comments section below! Additional Resources on the Addgene Blog...

...with limited spectral overlap are used together. Second, KTRs are easy to design. The key determinants ... activity in specific subcellular compartments. Second, because KTRs shuttle between the nucleus and the...

... public and help make science more transparent. Second, SITN helps graduate students develop their communication...engage the public - let us know in the comments section! More information about SITN: DayCon event website...

...eight modular repeats, each of which binds a consecutive base in its RNA target. By changing amino acids...specificity of other RNA binding domains depends on the secondary structure of RNA. As a result, targeting the RNA...

...This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News... get involved in science communication. In this second half of our conversation with Vini Mani and Amy...

...forces its insect host to dive into water. There, the worm emerges to find a mate, leaving the insect dead ...

...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

...DNA—to cut, nick, or bind. DNA CRISPR array: The section of the bacterial chromosome that stores a genetic...trans-activating CRISPR RNA” base pairs to the repeat section of the crRNA, forming the repeat:antirepeat duplex...

...and there are unique benefits to using sdAbs as secondary antibodies. sdAbs, however, really shine in vivo...the Addgene blog Introduction to Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic...

... beauty of serendipity often emerges at the intersections of disciplines. Cast your net wider, explore...Maintaining openness and looseness of spirit The second crucial element in harnessing the power of serendipity...

... This response is immediate, on the scale of milliseconds, and can precisely link a neuron's activation...controlled Unique advantages Mapping the brainDissecting circuits Studying GPCR pathways   Acknowledgements...

...ribozyme. In the second-generation SPARC2 system, HDVR is placed upstream of the second attP site to cleave...

...tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success with significantly... IgG, it is helpful to incubate with a rabbit secondary antibody when using a non-rabbit primary, prior...