We narrowed to 401 results for: caps
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TypeBlog Post... Primary cells recapitulate the natural biology of a cell type of interest better than immortalized lines...to a protein of interest thereby giving it the capacity to enter cells. One such CPP was discovered in...
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Clear and Accessible Writing
TypeBlog Post...densities are captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40%... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post... money, and viruses at low concentrations often escape detection. The Alexander Green lab found a solution...University of Toronto expanded the specificity landscape of the SH3 proteins using peptide-phage display... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post... 2.8 kb in size and are easy to package in low-capacity vectors! These small, but mighty type VI-D enzymes...Their small size makes then easy to package in low-capacity vectors like AAV, a popular vector due to its ... -
Viral Vectors 101: Viral Applications
TypeBlog Post...the viral titer (the number of viral particles capable of infecting a host cell) can be tuned so that ...Viral vector platforms within the gene therapy landscape. Sig Transduct Target Ther 6, 53 (2021). DOI: ... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post...Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency Transient...Mouse Cells and Hematopoietic Cells Low Packaging Capacity (~4.5kb From basic science to clinical applications... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...been trying to expand their precision editing capabilities. Base editors make specific DNA base changes...development towards faster and more precise base editors capable of editing previously uneditable sequences. Four... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...Flagship Ventures – a local Cambridge biotech venture capital firm with deep ties into the local academic community...Drug by Barry Werth Ten Tips for Raising Startup Capital in Biotech - Posted on Forbes.com, contributed... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...enzyme hydropersulfide-propagating enzymes 3-mercaptopyruvate sulfurtransferase (MPST) which catalyzes the... the desulfuration of 3-mercaptopyruvate and generates an enzyme-bound hydropersulfide that is then transferred... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post... therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made...Cas7 and Cas11 domains. Cas7-11 cuts ssRNA and is capable of pre-crRNA self-processing. Functionally, it ... -
CRISPRainbow and Genome Visualization
TypeBlog Post...fluorescent in situ hybridization (FISH) and chromosome capture suffer from low resolution and can’t be used in...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...). Bacteria need special fertility genes to be capable of conjugation, and these are encoded by certain...conserve energy, these specialized degradative capabilities are often tightly regulated to be active only... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...on the position in the protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions...ABE8s demonstrate an overall improved base editing capacity, even at sites that used to be difficult to target... -
The Scientific Conference Poster Session: Tips for Success
TypeBlog Post...you’re simply attending, but uncertain how to capitalize on your experience. No matter the reason for ...bullet points and elaborate if your audience seems captivated. Expertly navigate questions Answering questions... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...thin sheet to illuminate a fluorescent sample and captured the reflected light using a different objective...Ultramicroscope was developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...likely do! Give our summary table a look for a quick recap of the pros and cons of each antibiotic and happy...novel Escherichia coli protein expression strain capable of correctly folding disulfide bonded proteins ... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...would permit point mutations in RNA which could recapitulate or rescue known pathogenic alleles, or introduce...REPAIRv1 to merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...rough "COVID-19 effect" on order data, it does not capture the fact that different parts of the world encountered... each paper, respectively. The fitted model captures weekend, Christmas and New Years, and COVID-19... -
DNA Purification Without a Kit
TypeBlog Post...hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA, and filters can be stacked...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters. Image...