We narrowed to 310 results for: construct
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TypeBlog Post...to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...
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Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post... mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically labeling... efficiently in two-photon microscopy and new constructs have facilitated the creation of mouse lines ... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation for ...for next generation sequencing (NGS) library construction, and circularization of dsDNA (6). Less commonly... -
Lentivirus Plasmids
TypeCollection...glycoprotein (Indiana strain). Use with packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging...GP. Good for work in human MSCs. Use packaging construct encoding Tat. Reiser Return to Top Popular Lentiviral... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...Frankenbody,” like the infamous frankenstein, was constructed by grafting different parts together. By combining... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post...#104110 - #104112) (Figure 2). We’ve also created constructs with containing chemical tags such as the SNAP-tag...neurons for light microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling,... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...justifying each single element used to build the construct and explain why they selected a certain target... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...done painstakingly by hand. Records of plasmid construction were often incomplete or nonexistent. In the... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light... -
Another Pathway into Cells: iTOP
TypeBlog Post...primary cells. What if, instead of supplying a DNA construct, they could simply induce cells to take up protein... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...are comparatively small allowing multiple sgRNA constructs to be introduced simultaneously in a single plasmid... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...additional color variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for... -
Fluorescent Protein Guide
TypeCollection...biosensors - Pertz Fluorescent Protein Fusion Construct Set - Hamdoun Browse All Plasmid Kits Community... -
AAV Viral Preps
TypeCollection...Addgene's viral vectory packaging service. Browse constructs currently available in viral format.... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...however, suffer from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome...be directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA... -
TALEN Engineering
TypeCollection...transcriptional activator target sites and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast... -
Fluorescent Protein Guide: Biosensors
TypeCollection...conformational change in the sensing region of the construct leads to a change in signal of a circularly permuted...different binding affinities or negative-control constructs used to characterize the sensor's behavior in...biosensor in a novel context or modifying an existing construct, as fluorescence properties can be affected by...fluorescent protein biosensor for maltose Rapid construction of metabolite biosensors using domain-insertion...fluorescent protein biosensor for trehalose Rapid construction of metabolite biosensors using domain-insertion...for popular biosensors in different expression constructs? Browse the table below or search for a biosensor...Use the cpFRET kit from the Pertz laboratory to construct custom biosensors. The ScEnSor Kit from the Olsson... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...and New England Biolabs sponsored the capital construction of our new BL2+ laboratory and the infrastructure... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...response. To create this bacterial sensor, he will construct plasmids containing promoter regions that activate... -
DAP arrays for multiplex gene editing
TypeBlog Post...sequences can be used together in DAP array. To construct DAP array for multiplex gene editing applications...