We narrowed to 308 results for: construct

...protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and...

...compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed Addgene...

...cycles or low fidelity Taq DNA polymerase, the construction of specific sequences for base pair overhangs...

...PMCID: PMC2134509. Cohen, Stanley N. et al. “Construction of Biologically Functional Bacterial Plasmids...

...pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes...

...to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...

...vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation for ...for next generation sequencing (NGS) library construction, and circularization of dsDNA (6). Less commonly...

... mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically labeling... efficiently in two-photon microscopy and new constructs have facilitated the creation of mouse lines ...

...expression, and spike protein constructs for making VSV pseudo virus. Addgene blog Scientists...Reverse transcriptase, protease, and polymerase constructs for SARS-CoV-2 detection assays Neutralizing ...

...justifying each single element used to build the construct and explain why they selected a certain target...

...done painstakingly by hand. Records of plasmid construction were often incomplete or nonexistent. In the...

...Frankenbody,” like the infamous frankenstein, was constructed by grafting different parts together. By combining...

...#104110 - #104112) (Figure 2). We’ve also created constructs with containing chemical tags such as the SNAP-tag...neurons for light microscopy-based neuronal reconstruction. To achieve stochastic multicolor labeling,...

...live animal. They started by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light...

...primary cells. What if, instead of supplying a DNA construct, they could simply induce cells to take up protein...

...are comparatively small allowing multiple sgRNA constructs to be introduced simultaneously in a single plasmid...

...additional color variants. This resulted in the construction of redCALWY-1, a red-shifted FRET sensor for...

... Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode: Injection and ...triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative selection marker); pGH8 (neuronal...

...however, suffer from many pitfalls: they require construction of plasmid vectors and/or rely on cumbersome...be directly purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA...

... voltage changes in worms and have made these constructs available at Addgene. These include Arch(D95N...