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We narrowed to 200 results for: crispr cas9 grna plasmid

Showing: 161 - 180 of 200 results

  1. Improved Plasmid Maps Powered by SnapGene

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    Blog Post
    ...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display, ...easier for you to share plasmid information with colleagues, or explain plasmid features to a lab member... on the main plasmid page under the "Resource Information" section. Analyze your plasmid sequence For ...over from our users: "Please, please improve your plasmid maps!" After thoughtful design, vetting, and tweaking...tweaking, we’re excited to announce that our plasmid and sequence displays are now powered by GSL Biotech's...software and extensive feature library, our updated plasmid and sequence displays are now much easier to interpret...

  2. Worm Expression Resources

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    Collection
    ... Plasmid Collections Worm Plasmids Worm Plasmids and Resources Plasmid Tools Worm Constructs... insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR plasmids from John Calarco's...elegans via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools for ...Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR resources ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some ...Browse plasmid tools, kits, and resources (such as CRISPR and synthetic biology collections) for use ...

  3. CRISPR Pooled gRNA Libraries

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    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is ... CRISPR Pooled Libraries CRISPR Pooled Libraries Browse CRISPR Plasmids By Function Genome...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening experiment...primary cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells...PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson DNA Repair CRISPRi Libraries 177663, 177664...Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies CRISPR/Cas9-assisted...

  4. Hot Plasmids Spring 2024

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...pooled CRISPR screens By Emily Bentley Building on their CROPseq method for optical pooled CRISPR screening...has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton et...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative...Huntington’s Disease, including plasmids, antibodies, viral vector preps, CRISPR tools, and more.  ...stabilizing the 3’ end of the pegRNA, which is not well protected by Cas9. This is similar to the proposed...for a range of applications, including CRISPR activation by dCas9-VPR, prime editing, and delivery of disease-relevant...

  5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post
    ...Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... our hot plasmid articles from April 2018. If you'd ever like to write about a recent plasmid deposit ...Inducible Recruitment for Epigenome editing by Cas9 (FIRE–Cas9), combines these approaches to enable rapid...epigenetic memory at nearly any locus. The FIRE-Cas9 plasmids can be found here. Braun et al. Nat Communications...epigenetic modulation. While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules...

  6. Zebrafish Plasmid Collection

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    ... zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones....Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference... Plasmid Collections Zebrafish Plasmids Zebrafish Plasmids and Resources... of the plasmids and tools available at Addgene for use in zebrafish. Browse Plasmids Plasmid Tools for...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...

  7. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ... Encodes gRNA/shRNA A plasmid with one or more gRNA or shRNA sequences cloned into it. gRNAs are used...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

  8. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ... in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab... the vector and distinguish between plasmids that contain the gRNA (white) from those that do not (blue...once in the plasmid backbone and another enzyme that cuts within the insert. If the plasmid contains the... PCR product if the plasmid contains the insert and no PCR product if the plasmid does not contain the...resources on the Addgene blog Get all the plasmid basics in our Plasmids 101 series Find more molecular biology...If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone...clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to begin ...

  9. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...Addgene.org Visit our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing ... the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein to recognize... Find more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing... The new frontier of genome engineering with CRISPR-Cas9. Science 346:1258096 . https://doi.org/10.1126... Zhang F (2013) Genome engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https:...between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing...during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the DNA, the ...

  10. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources ...s12915-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...without end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for...other pneumococcal strains. The BARBEKO sgRNA library uses CRISPR cytosine base editors to disrupt gene ...A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use viral preps   Improved...

  11. Analyzing CRISPR Editing Results with ICE from Synthego

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    Blog Post
    ... Check out our CRISPR guide Find CRISPR plasmids for your research Find other CRISPR software resources... from single or multiplex CRISPR-Cas9 double-strand DNA breaks using SpCas9. More features will be added...Addgene Blog Browse our CRISPR featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility... Guide for Analyzing CRISPR Editing Results with ICE on Synthego’s blog. CRISPR-based genome engineering...Inference of CRISPR Edits) to solve this problem. This tool was initially created to support the CRISPR analysis...successful CRISPR experiments. Jessica is a sci-fi enthusiast, and when she is not guiding CRISPR researchers...Currently, ICE is configured for Cas9 fromStreptococcus pyogenes (SpCas9). ICE Score - The editing efficiency...

  12. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post
    ... Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  ...Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed. Find the Plasmids @Addgene: ...Modifying the CRISPRi system for multidirectional control The CRISPR interference (CRISPRi) model consists...consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable...allowing multiple sgRNA constructs to be introduced simultaneously in a single plasmid. The functionality...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to...metabolic biofactories by demonstrating the use of CRISPR-based RNA scaffolds to mimic natural transcriptional...

  13. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ...reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of interest....with some plasmids. You just need three plasmids to start making AAV:  the packaging plasmid which contains... transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids have two ...of an AAV transfer plasmid. Parts shown in green are key elements of an AAV plasmid. Parts shown in grey... elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal ...transfer plasmids have a promoter, but this element isn’t required when the transfer plasmid harbors non-coding...Transfer Plasmid video!   Now you’ve got a basic understanding of some common AAV plasmid elements!...

  14. Hot Plasmids February 2024

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did...facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression yielded editing of 80–90%... of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long, negatively charged sgRNA), RNP...Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral sgRNA targeting mCherry reporter (mChe)...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ...multiplexed guides. Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization...

  15. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...nuclease-deficient Cas9 can bind loci in the genome that are defined by a gRNA. Since Cas9 is a protein, a...genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed...protein fusions Browse the Fluorescent Proteins Plasmid Kits ...

  16. Viral Vectors 101: Viral Applications

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    Blog Post
    ... depending on the vector and virus. Viral CRISPR-Cas9 tools have been engineered to introduce frameshift...Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...sequence for degradation. Viral introduction of shRNA plasmids can induce gene-specific silencing temporarily...transfection and random genomic integration of a plasmid. Viral transduction is also generally less toxic...shRNA sequence specific to your gene’s mRNA or a sgRNA targeting an exon in your gene of interest, for ...with many applications. These libraries include sgRNAs targeting the entire genome, overexpression of ...

  17. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...Get the basics about CRISPR in our CRISPR guide Browse our entire COVID-19 plasmid collection ...technologies like CRISPR. Figure 1: An overview of the general schematic of CRISPR/Cas based COVID...check out this table.  All-in-one dual CRISPR/Cas12a (AIOD-CRISPR) assay – for rapid, ultrasensitive and...detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay Sensitivity:10-100 copies of...Lucia et al., 2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​s​ensitivity ​E​nzymatic...the case of HIV-1 and SARS-CoV-2 detection, AIOD-CRISPR without preamplification was able to detect as ... Cas13, upon finding complementarity between the gRNA and a given fluorescently labelled substrate, gets...

  18. CRISPR Plasmids - Repress Gene Expression

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Interfere CRISPR Plasmids: Interfere...If the plasmid you’re using does not also express a gRNA, you will need to use a separate gRNA expression...search the tables below for CRISPR plasmids for transcriptional inhibition. Plasmids are available for expression...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Interfere Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  19. CRISPR Plasmids - Activate Gene Expression

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    ...Activate CRISPR Plasmids: Activate Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...If the plasmid that you choose does not also express a gRNA, you will need to use a separate gRNA expression...search the tables below for CRISPR plasmids for transcriptional activation. Plasmids are available for expression...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene/Insert...

  20. CRISPR Plasmids and Resources

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    Collection
    ...preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of plasmids that are used ...variety of factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in ... . Drosophila CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for ...systems. Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed for use... in plants. Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed for use ...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...
Showing: 161 - 180 of 200 results