...). Created with BioRender.com.
Before using gRNAs in vivo, they should be screened for activity in...transcription at genomic sites targeted by specific sgRNAs. Its RNA-guided nature makes CRISPRa much simpler...all the protein and RNA components, and several sgRNAs must be screened to identify effective ones.
Simplified...constrained by AAV packaging limits (i.e., how many sgRNA expression cassettes will fit).
Cre-dependent expression...expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse an attractive tool to...Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs can be found here.
Neuron-optimized viral vectors...lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression...
